Cover Image

Differentiation of salmonella enterica based on PCR detection of selected somatic and flagellar antigens

Serotyping is the basis of Salmonella surveillance. However, the limitations of the traditional serotyping have stimulated rapid research and development in DNA-based serotyping. The aim of this study was to apply a combination of sequential multiplex PCRs targeting the O, H and Vi antigens to serot...

Full description

Saved in:
Bibliographic Details
Main Authors: Nori, M.E.E., Thong, Kwai Lin
Format: Article
Language:English
Published: 2010
Subjects:
Q Science (General)
QR Microbiology
Online Access:http://eprints.um.edu.my/5515/1/Differentiation_of_Salmonella_enterica_based_on_PCR_detection_of_selected_somatic_and_flagellar_antigens.pdf
http://eprints.um.edu.my/5515/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.um.eprints.5515
record_format eprints
spelling my.um.eprints.55152018-10-15T04:23:15Z http://eprints.um.edu.my/5515/ Differentiation of salmonella enterica based on PCR detection of selected somatic and flagellar antigens Nori, M.E.E. Thong, Kwai Lin Q Science (General) QR Microbiology Serotyping is the basis of Salmonella surveillance. However, the limitations of the traditional serotyping have stimulated rapid research and development in DNA-based serotyping. The aim of this study was to apply a combination of sequential multiplex PCRs targeting the O, H and Vi antigens to serotype a panel of 122 recently isolated human- and foodborne-Salmonella strains. The mPCR that targets the o (genes wzxC2, rfbJ, prt, tyv, wzxE, wzxC1, prt) and Vi (viaB) antigens successfully subtyped the strains into serogroups C2 (n = 35, 28.7), B (n = 33, 27.1), D9 (n = 29, 22.9), E (n = 21, 17.2), C1 (n = 2, 64) and A (n = 2, 1.64). Eight of the Salmonella strains from serogroup D were positive for Vi antigen. Two multiplex PCRs were optimized for detection of H1 antigens (Ha, Hb, Hd, r, z(10), z(6), g and m) and H2 antigens (1.5, 1.2, 1.6 and enx). Overall, the multiplex PCRs of O, H and Vi antigens results correctly serotyped 94 of 122 strains (77). The most frequent serovars encountered were Salmonella weltevrerden, Salmonella enteritidis, Salmonella typhimurium, Salmonella hadar and Salmonella typhi. Application of DNA based technique for serogrouping and serotyping of the selected Salmonella enterica was found to be robust, quick, specific and reliable for the specific antigenic targets and is useful in the study area which lack complete serotyping facilities. 2010 Article PeerReviewed application/pdf en http://eprints.um.edu.my/5515/1/Differentiation_of_Salmonella_enterica_based_on_PCR_detection_of_selected_somatic_and_flagellar_antigens.pdf Nori, M.E.E. and Thong, Kwai Lin (2010) Differentiation of salmonella enterica based on PCR detection of selected somatic and flagellar antigens. African Journal of Microbiology Research, 4 (9). pp. 871-876. ISSN 1996-0808
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic Q Science (General)
QR Microbiology
spellingShingle Q Science (General)
QR Microbiology
Nori, M.E.E.
Thong, Kwai Lin
Differentiation of salmonella enterica based on PCR detection of selected somatic and flagellar antigens
description Serotyping is the basis of Salmonella surveillance. However, the limitations of the traditional serotyping have stimulated rapid research and development in DNA-based serotyping. The aim of this study was to apply a combination of sequential multiplex PCRs targeting the O, H and Vi antigens to serotype a panel of 122 recently isolated human- and foodborne-Salmonella strains. The mPCR that targets the o (genes wzxC2, rfbJ, prt, tyv, wzxE, wzxC1, prt) and Vi (viaB) antigens successfully subtyped the strains into serogroups C2 (n = 35, 28.7), B (n = 33, 27.1), D9 (n = 29, 22.9), E (n = 21, 17.2), C1 (n = 2, 64) and A (n = 2, 1.64). Eight of the Salmonella strains from serogroup D were positive for Vi antigen. Two multiplex PCRs were optimized for detection of H1 antigens (Ha, Hb, Hd, r, z(10), z(6), g and m) and H2 antigens (1.5, 1.2, 1.6 and enx). Overall, the multiplex PCRs of O, H and Vi antigens results correctly serotyped 94 of 122 strains (77). The most frequent serovars encountered were Salmonella weltevrerden, Salmonella enteritidis, Salmonella typhimurium, Salmonella hadar and Salmonella typhi. Application of DNA based technique for serogrouping and serotyping of the selected Salmonella enterica was found to be robust, quick, specific and reliable for the specific antigenic targets and is useful in the study area which lack complete serotyping facilities.
format Article
author Nori, M.E.E.
Thong, Kwai Lin
author_facet Nori, M.E.E.
Thong, Kwai Lin
author_sort Nori, M.E.E.
title Differentiation of salmonella enterica based on PCR detection of selected somatic and flagellar antigens
title_short Differentiation of salmonella enterica based on PCR detection of selected somatic and flagellar antigens
title_full Differentiation of salmonella enterica based on PCR detection of selected somatic and flagellar antigens
title_fullStr Differentiation of salmonella enterica based on PCR detection of selected somatic and flagellar antigens
title_full_unstemmed Differentiation of salmonella enterica based on PCR detection of selected somatic and flagellar antigens
title_sort differentiation of salmonella enterica based on pcr detection of selected somatic and flagellar antigens
publishDate 2010
url http://eprints.um.edu.my/5515/1/Differentiation_of_Salmonella_enterica_based_on_PCR_detection_of_selected_somatic_and_flagellar_antigens.pdf
http://eprints.um.edu.my/5515/
_version_ 1643687597219774464
score 13.211869

Similar Items