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Characterisation of the Virulence Factors and Genetic Types of Methicillin Susceptible Staphylococcus aureus from Patients and Healthy Individuals

Methicillin sensitive Staphylococcus aureus is an important bacterial pathogen associated with hospitaland community-acquired infections leading to endocarditis, skin tissue infection and pneumonia. The objective of this study was to determine both the genetic characteristics of methicillin-sensitiv...

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Main Authors:	Lim, K.T., Hanifah, Y.A., Yusof, M.Y.M., Thong, Kwai Lin
Format:	Article
Published:	2012
Subjects:	Q Science (General) QR Microbiology
Online Access:	http://eprints.um.edu.my/5457/
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spelling	my.um.eprints.54572018-10-15T05:10:21Z http://eprints.um.edu.my/5457/ Characterisation of the Virulence Factors and Genetic Types of Methicillin Susceptible Staphylococcus aureus from Patients and Healthy Individuals Lim, K.T. Hanifah, Y.A. Yusof, M.Y.M. Thong, Kwai Lin Q Science (General) QR Microbiology Methicillin sensitive Staphylococcus aureus is an important bacterial pathogen associated with hospitaland community-acquired infections leading to endocarditis, skin tissue infection and pneumonia. The objective of this study was to determine both the genetic characteristics of methicillin-sensitive S. aureus (MSSA) strains, and the occurrence of virulence factors produced by S. aureus strains isolated from UMMC and healthy students in the University from year 2009. Out of 429 nasal swab samples, 67 were MSSA. The prevalence of 21 different virulence genes among 67 Malaysian clinical and community MSSA strains was determined by PCR, and their genetic features were assessed by PCR-RFLP of coa gene, agr types, spa typing and PFGE. The five predominant virulence genes were ica (79 ), efb and fnbA (61 each), sdrE (57 ) and hlg (45 ). Toxin genes (enterotoxin, etd and pvl) were significantly more common (P\0.05) in clinical strains compared to community strains. Three agr genotypes were observed: agr type I (45 ), agr type III (25 ) and agr type II (19 ). All 67 MSSA strains were distinguished into 26 profiles by PCR-RFLP of coa, 55 pulsotypes and 21 spa types. Four novel spa types (t7312, t7581, t7582 and t7583) were observed. In conclusion, different virulence profiles were observed in MSSA strains in Malaysia where toxin genes were more prevalent among clinical strains. No correlation between DNA profiles (coa- RFLP, PFGE and spa) and virulotypes was observed. The Malaysian MSSA strains from clinical and community sources were genetically diverse and heterogeneous 2012 Article PeerReviewed Lim, K.T. and Hanifah, Y.A. and Yusof, M.Y.M. and Thong, Kwai Lin (2012) Characterisation of the Virulence Factors and Genetic Types of Methicillin Susceptible Staphylococcus aureus from Patients and Healthy Individuals. Indian Journal of Microbiology. pp. 1-8. ISSN 0046-8991 10.1007/s12088-012-0286-7
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	Q Science (General) QR Microbiology
spellingShingle	Q Science (General) QR Microbiology Lim, K.T. Hanifah, Y.A. Yusof, M.Y.M. Thong, Kwai Lin Characterisation of the Virulence Factors and Genetic Types of Methicillin Susceptible Staphylococcus aureus from Patients and Healthy Individuals
description	Methicillin sensitive Staphylococcus aureus is an important bacterial pathogen associated with hospitaland community-acquired infections leading to endocarditis, skin tissue infection and pneumonia. The objective of this study was to determine both the genetic characteristics of methicillin-sensitive S. aureus (MSSA) strains, and the occurrence of virulence factors produced by S. aureus strains isolated from UMMC and healthy students in the University from year 2009. Out of 429 nasal swab samples, 67 were MSSA. The prevalence of 21 different virulence genes among 67 Malaysian clinical and community MSSA strains was determined by PCR, and their genetic features were assessed by PCR-RFLP of coa gene, agr types, spa typing and PFGE. The five predominant virulence genes were ica (79 ), efb and fnbA (61 each), sdrE (57 ) and hlg (45 ). Toxin genes (enterotoxin, etd and pvl) were significantly more common (P\0.05) in clinical strains compared to community strains. Three agr genotypes were observed: agr type I (45 ), agr type III (25 ) and agr type II (19 ). All 67 MSSA strains were distinguished into 26 profiles by PCR-RFLP of coa, 55 pulsotypes and 21 spa types. Four novel spa types (t7312, t7581, t7582 and t7583) were observed. In conclusion, different virulence profiles were observed in MSSA strains in Malaysia where toxin genes were more prevalent among clinical strains. No correlation between DNA profiles (coa- RFLP, PFGE and spa) and virulotypes was observed. The Malaysian MSSA strains from clinical and community sources were genetically diverse and heterogeneous
format	Article
author	Lim, K.T. Hanifah, Y.A. Yusof, M.Y.M. Thong, Kwai Lin
author_facet	Lim, K.T. Hanifah, Y.A. Yusof, M.Y.M. Thong, Kwai Lin
author_sort	Lim, K.T.
title	Characterisation of the Virulence Factors and Genetic Types of Methicillin Susceptible Staphylococcus aureus from Patients and Healthy Individuals
title_short	Characterisation of the Virulence Factors and Genetic Types of Methicillin Susceptible Staphylococcus aureus from Patients and Healthy Individuals
title_full	Characterisation of the Virulence Factors and Genetic Types of Methicillin Susceptible Staphylococcus aureus from Patients and Healthy Individuals
title_fullStr	Characterisation of the Virulence Factors and Genetic Types of Methicillin Susceptible Staphylococcus aureus from Patients and Healthy Individuals
title_full_unstemmed	Characterisation of the Virulence Factors and Genetic Types of Methicillin Susceptible Staphylococcus aureus from Patients and Healthy Individuals
title_sort	characterisation of the virulence factors and genetic types of methicillin susceptible staphylococcus aureus from patients and healthy individuals
publishDate	2012
url	http://eprints.um.edu.my/5457/
_version_	1643687581825630208
score	13.211869

Characterisation of the Virulence Factors and Genetic Types of Methicillin Susceptible Staphylococcus aureus from Patients and Healthy Individuals

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