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Determination of albendazole, fenbendazole, and their metabolites in mouse plasma by high performance liquid chromatography using fluorescence and ultraviolet detection

A rapid and reliable high performance liquid chromatographic (HPLC) method has been developed and validated for the determination of albendazole (ABZ), albendazole sulfoxide (ABZSO), albendazole sulfone (ABZSO(2)); fenbendazole (FBZ), fenbendazole sulfoxide (FBZSO), and fenbendazole sulfone (FBZSO(2...

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Main Authors: Rummel, N., Chung, I., Shaikh, B.
Format: Article
Language:English
Published: 2011
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/5073/1/Rummel-2011-DETERMINATION_OF_ALB.pdf
http://eprints.um.edu.my/5073/
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spelling my.um.eprints.50732013-03-19T00:32:36Z http://eprints.um.edu.my/5073/ Determination of albendazole, fenbendazole, and their metabolites in mouse plasma by high performance liquid chromatography using fluorescence and ultraviolet detection Rummel, N. Chung, I. Shaikh, B. R Medicine A rapid and reliable high performance liquid chromatographic (HPLC) method has been developed and validated for the determination of albendazole (ABZ), albendazole sulfoxide (ABZSO), albendazole sulfone (ABZSO(2)); fenbendazole (FBZ), fenbendazole sulfoxide (FBZSO), and fenbendazole sulfone (FBZSO(2)) in mouse plasma. The mouse plasma was made alkaline with potassium carbonate and extracted with ethylacetate. The extracts were evaporated, reconstituted in mobile phase, and analyzed by HPLC. The chromatography was carried out on a reversed-phase column using acetonitrile-methanol-buffer as the mobile phase. The fenbendazole and its metabolites were detected by using UV detector set at the wavelength of 290 nm. The albendazole and its metabolites were detected by a fluorescence detector with excitation and emission wavelengths of 290 and 330 nm, respectively. The average recoveries of ABZ, ABZSO, and ABZSO(2) from fortified control plasma samples were 95, 82, and 92, respectively. Similarly, the average recoveries of FBZ, FBZSO, and FBZSO(2) were 64, 90, and 94, respectively. The average CVs were <= 15 for all of the compounds. The method was applied to incurred mouse plasma samples to determine ABZ, FBZ, and their metabolites. 2011 Article PeerReviewed application/pdf en http://eprints.um.edu.my/5073/1/Rummel-2011-DETERMINATION_OF_ALB.pdf Rummel, N. and Chung, I. and Shaikh, B. (2011) Determination of albendazole, fenbendazole, and their metabolites in mouse plasma by high performance liquid chromatography using fluorescence and ultraviolet detection. Journal of Liquid Chromatography & Related Technologies, 34 (18). pp. 2211-2223. ISSN 1082-6076
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic R Medicine
spellingShingle R Medicine
Rummel, N.
Chung, I.
Shaikh, B.
Determination of albendazole, fenbendazole, and their metabolites in mouse plasma by high performance liquid chromatography using fluorescence and ultraviolet detection
description A rapid and reliable high performance liquid chromatographic (HPLC) method has been developed and validated for the determination of albendazole (ABZ), albendazole sulfoxide (ABZSO), albendazole sulfone (ABZSO(2)); fenbendazole (FBZ), fenbendazole sulfoxide (FBZSO), and fenbendazole sulfone (FBZSO(2)) in mouse plasma. The mouse plasma was made alkaline with potassium carbonate and extracted with ethylacetate. The extracts were evaporated, reconstituted in mobile phase, and analyzed by HPLC. The chromatography was carried out on a reversed-phase column using acetonitrile-methanol-buffer as the mobile phase. The fenbendazole and its metabolites were detected by using UV detector set at the wavelength of 290 nm. The albendazole and its metabolites were detected by a fluorescence detector with excitation and emission wavelengths of 290 and 330 nm, respectively. The average recoveries of ABZ, ABZSO, and ABZSO(2) from fortified control plasma samples were 95, 82, and 92, respectively. Similarly, the average recoveries of FBZ, FBZSO, and FBZSO(2) were 64, 90, and 94, respectively. The average CVs were <= 15 for all of the compounds. The method was applied to incurred mouse plasma samples to determine ABZ, FBZ, and their metabolites.
format Article
author Rummel, N.
Chung, I.
Shaikh, B.
author_facet Rummel, N.
Chung, I.
Shaikh, B.
author_sort Rummel, N.
title Determination of albendazole, fenbendazole, and their metabolites in mouse plasma by high performance liquid chromatography using fluorescence and ultraviolet detection
title_short Determination of albendazole, fenbendazole, and their metabolites in mouse plasma by high performance liquid chromatography using fluorescence and ultraviolet detection
title_full Determination of albendazole, fenbendazole, and their metabolites in mouse plasma by high performance liquid chromatography using fluorescence and ultraviolet detection
title_fullStr Determination of albendazole, fenbendazole, and their metabolites in mouse plasma by high performance liquid chromatography using fluorescence and ultraviolet detection
title_full_unstemmed Determination of albendazole, fenbendazole, and their metabolites in mouse plasma by high performance liquid chromatography using fluorescence and ultraviolet detection
title_sort determination of albendazole, fenbendazole, and their metabolites in mouse plasma by high performance liquid chromatography using fluorescence and ultraviolet detection
publishDate 2011
url http://eprints.um.edu.my/5073/1/Rummel-2011-DETERMINATION_OF_ALB.pdf
http://eprints.um.edu.my/5073/
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score 13.211869

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