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Proteomics Studies Indicate Differential Regulation of Key Metabolism, Energy, and Stress-Responsive Proteins in Azadirachta indica Callus Upon Exposure to Salinity and Jasmonic Acid Treatments

Azadirachta indica or neem is recognized for its unique properties, both against insects and in improving human health. However, proteomics studies of this plant's response to abiotic stresses have yet to be reported. Application of this technique highly depends on efficient and optimized sampl...

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Main Authors: Ashokhan, Sharmilla, Karsani, Saiful Anuar, Yaacob, Jamilah Syafawati
Format: Article
Published: Springer 2024
Subjects:
QH Natural history
SB Plant culture
Online Access:http://eprints.um.edu.my/47166/
https://doi.org/10.1007/s00344-023-11065-1
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spelling my.um.eprints.471662024-12-30T04:37:41Z http://eprints.um.edu.my/47166/ Proteomics Studies Indicate Differential Regulation of Key Metabolism, Energy, and Stress-Responsive Proteins in Azadirachta indica Callus Upon Exposure to Salinity and Jasmonic Acid Treatments Ashokhan, Sharmilla Karsani, Saiful Anuar Yaacob, Jamilah Syafawati QH Natural history SB Plant culture Azadirachta indica or neem is recognized for its unique properties, both against insects and in improving human health. However, proteomics studies of this plant's response to abiotic stresses have yet to be reported. Application of this technique highly depends on efficient and optimized sample preparation, in deriving highly reproducible proteomics data. The present study highlights the comparison of protein extraction method which effectively recovers proteins from recalcitrant phenolic-rich tissues of neem callus. Four protocols based on TCA-acetone or/and phenol extraction methods were compared. The TCA-acetone complemented with PVPP method was the most effective approach and generated the greatest total protein yields, spot number, good resolution, and spot intensities. Subsequently, the differential proteomics performed revealed that proteins associated with oxidoreduction, energy, transcriptional, stress response, respiration, and cell division were differentially regulated in salinity stress treatment. Meanwhile, the expression of proteins classified in the group of energy, photosynthesis, protein synthesis, oxidoreduction, and post-transcriptional regulation was altered in the JA-stressed callus. The accumulation of AZA was also noticeably reduced in salinity- and JA-treated callus, suggesting these tested stress parameters were not effective in improving AZA production in the neem callus. Overall, this analysis provides valuable information on the application of plant tissue culture as an alternative for sustainable production of major bioactive constituents in A. indica. Together, the proteomics results brought forward a possible fundamental representation of the mechanism associated with salinity and JA tolerance in the neem callus. Springer 2024-09 Article PeerReviewed Ashokhan, Sharmilla and Karsani, Saiful Anuar and Yaacob, Jamilah Syafawati (2024) Proteomics Studies Indicate Differential Regulation of Key Metabolism, Energy, and Stress-Responsive Proteins in Azadirachta indica Callus Upon Exposure to Salinity and Jasmonic Acid Treatments. Journal of Plant Growth Regulation, 43 (9, SI). pp. 3119-3139. ISSN 0721-7595, DOI https://doi.org/10.1007/s00344-023-11065-1 <https://doi.org/10.1007/s00344-023-11065-1>. https://doi.org/10.1007/s00344-023-11065-1 10.1007/s00344-023-11065-1
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QH Natural history
SB Plant culture
spellingShingle QH Natural history
SB Plant culture
Ashokhan, Sharmilla
Karsani, Saiful Anuar
Yaacob, Jamilah Syafawati
Proteomics Studies Indicate Differential Regulation of Key Metabolism, Energy, and Stress-Responsive Proteins in Azadirachta indica Callus Upon Exposure to Salinity and Jasmonic Acid Treatments
description Azadirachta indica or neem is recognized for its unique properties, both against insects and in improving human health. However, proteomics studies of this plant's response to abiotic stresses have yet to be reported. Application of this technique highly depends on efficient and optimized sample preparation, in deriving highly reproducible proteomics data. The present study highlights the comparison of protein extraction method which effectively recovers proteins from recalcitrant phenolic-rich tissues of neem callus. Four protocols based on TCA-acetone or/and phenol extraction methods were compared. The TCA-acetone complemented with PVPP method was the most effective approach and generated the greatest total protein yields, spot number, good resolution, and spot intensities. Subsequently, the differential proteomics performed revealed that proteins associated with oxidoreduction, energy, transcriptional, stress response, respiration, and cell division were differentially regulated in salinity stress treatment. Meanwhile, the expression of proteins classified in the group of energy, photosynthesis, protein synthesis, oxidoreduction, and post-transcriptional regulation was altered in the JA-stressed callus. The accumulation of AZA was also noticeably reduced in salinity- and JA-treated callus, suggesting these tested stress parameters were not effective in improving AZA production in the neem callus. Overall, this analysis provides valuable information on the application of plant tissue culture as an alternative for sustainable production of major bioactive constituents in A. indica. Together, the proteomics results brought forward a possible fundamental representation of the mechanism associated with salinity and JA tolerance in the neem callus.
format Article
author Ashokhan, Sharmilla
Karsani, Saiful Anuar
Yaacob, Jamilah Syafawati
author_facet Ashokhan, Sharmilla
Karsani, Saiful Anuar
Yaacob, Jamilah Syafawati
author_sort Ashokhan, Sharmilla
title Proteomics Studies Indicate Differential Regulation of Key Metabolism, Energy, and Stress-Responsive Proteins in Azadirachta indica Callus Upon Exposure to Salinity and Jasmonic Acid Treatments
title_short Proteomics Studies Indicate Differential Regulation of Key Metabolism, Energy, and Stress-Responsive Proteins in Azadirachta indica Callus Upon Exposure to Salinity and Jasmonic Acid Treatments
title_full Proteomics Studies Indicate Differential Regulation of Key Metabolism, Energy, and Stress-Responsive Proteins in Azadirachta indica Callus Upon Exposure to Salinity and Jasmonic Acid Treatments
title_fullStr Proteomics Studies Indicate Differential Regulation of Key Metabolism, Energy, and Stress-Responsive Proteins in Azadirachta indica Callus Upon Exposure to Salinity and Jasmonic Acid Treatments
title_full_unstemmed Proteomics Studies Indicate Differential Regulation of Key Metabolism, Energy, and Stress-Responsive Proteins in Azadirachta indica Callus Upon Exposure to Salinity and Jasmonic Acid Treatments
title_sort proteomics studies indicate differential regulation of key metabolism, energy, and stress-responsive proteins in azadirachta indica callus upon exposure to salinity and jasmonic acid treatments
publisher Springer
publishDate 2024
url http://eprints.um.edu.my/47166/
https://doi.org/10.1007/s00344-023-11065-1
_version_ 1819909894495535104
score 13.223943

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