Staff View: Medicinal Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) Sclerotia Supplementation Mitigates Hypertension and Alleviates Vascular Dysfunction Partly through Oxidative Stress Modulation in Spontaneously Hypertensive Rats

Medicinal Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) Sclerotia Supplementation Mitigates Hypertension and Alleviates Vascular Dysfunction Partly through Oxidative Stress Modulation in Spontaneously Hypertensive Rats

Hypertension is a risk factor for cardiovascular diseases such as coronary artery disease, heart failure, and stroke. Lignosus rhinocerus (Cooke) Ryvarden (also known as tiger milk mushroom), has been reported to exhibit a range of pharmacological effects, such as anti-inflammatory, anti-proliferati...

Full description

Saved in:

Bibliographic Details
Main Authors:	Wong, Yi En, Razif, Muhammad Fazril Mohamad, Ng, Szu-Ting, Tan, Chon-Seng, Fung, Shin-Yee, Murugan, Dharmani Devi
Format:	Article
Published:	Begell House 2024
Subjects:	R Medicine (General)
Online Access:	http://eprints.um.edu.my/47125/ https://doi.org/10.1615/IntJMedMushrooms.2024055061
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my.um.eprints.47125
record_format	eprints
spelling	my.um.eprints.471252024-11-28T05:02:44Z http://eprints.um.edu.my/47125/ Medicinal Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) Sclerotia Supplementation Mitigates Hypertension and Alleviates Vascular Dysfunction Partly through Oxidative Stress Modulation in Spontaneously Hypertensive Rats Wong, Yi En Razif, Muhammad Fazril Mohamad Ng, Szu-Ting Tan, Chon-Seng Fung, Shin-Yee Murugan, Dharmani Devi R Medicine (General) Hypertension is a risk factor for cardiovascular diseases such as coronary artery disease, heart failure, and stroke. Lignosus rhinocerus (Cooke) Ryvarden (also known as tiger milk mushroom), has been reported to exhibit a range of pharmacological effects, such as anti-inflammatory, anti-proliferative, antioxidative, immunomodulatory and anti-asthmatic activities. Thus far, there is limited research that has explored its ability to mediate vascular effects in vivo. Therefore, this study investigated the antihypertensive and vascular protective effects of L. rhinocerus TM02 (R) sclerotia supplementation in spontaneously hypertensive rats (SHR). Wistar Kyoto (WKY) rats served as a normo-tensive control group. SHR were orally administered with L. rhinocerus TM02 (R) sclerotia (100 mg/kg and 300 mg/kg, respectively) for 8 weeks, and blood pressure was monitored every 2 weeks. Vascular function was evaluated using an organ bath (aorta) and wire myograph (renal artery) at the treatment endpoint. The levels of reactive oxygen species (ROS) and nitric oxide (NO) in the aorta and renal artery were evaluated using dihydroethidium (DHE) and difluoro fluorescein acetate (DAF-FM) fluorescence assays, respectively. Total plasma nitrate/nitrite and tumor necrosis factor alpha (TNF-alpha) levels were evaluated via colorimetric assays. In vivo treatment with L. rhinocerus TM02 (R) sclerotia sig-nificantly attenuated the increase in systolic blood pressure (SBP). It also alleviated vascular dysfunction and decreased elevated ROS in the aorta and renal arteries of the treated SHRs. Moreover, L. rhinocerus TM02 (R) sclerotia attenuated plasma TNF-alpha level but increased total plasma nitrate/nitrite, albeit slightly, coupled with significantly increased NO at the vascular level. Collectively, the present study demonstrated that L. rhinocerus TM02 (R) sclerotia supplementation exerted blood pressure lowering effects, partly attributed to improvements in vascular function via reduction in vascular oxidative stress. Begell House 2024 Article PeerReviewed Wong, Yi En and Razif, Muhammad Fazril Mohamad and Ng, Szu-Ting and Tan, Chon-Seng and Fung, Shin-Yee and Murugan, Dharmani Devi (2024) Medicinal Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) Sclerotia Supplementation Mitigates Hypertension and Alleviates Vascular Dysfunction Partly through Oxidative Stress Modulation in Spontaneously Hypertensive Rats. International Journal of Medicinal Mushrooms, 26 (11). pp. 27-40. ISSN 1521-9437, DOI https://doi.org/10.1615/IntJMedMushrooms.2024055061 <https://doi.org/10.1615/IntJMedMushrooms.2024055061>. https://doi.org/10.1615/IntJMedMushrooms.2024055061 10.1615/IntJMedMushrooms.2024055061
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	R Medicine (General)
spellingShingle	R Medicine (General) Wong, Yi En Razif, Muhammad Fazril Mohamad Ng, Szu-Ting Tan, Chon-Seng Fung, Shin-Yee Murugan, Dharmani Devi Medicinal Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) Sclerotia Supplementation Mitigates Hypertension and Alleviates Vascular Dysfunction Partly through Oxidative Stress Modulation in Spontaneously Hypertensive Rats
description	Hypertension is a risk factor for cardiovascular diseases such as coronary artery disease, heart failure, and stroke. Lignosus rhinocerus (Cooke) Ryvarden (also known as tiger milk mushroom), has been reported to exhibit a range of pharmacological effects, such as anti-inflammatory, anti-proliferative, antioxidative, immunomodulatory and anti-asthmatic activities. Thus far, there is limited research that has explored its ability to mediate vascular effects in vivo. Therefore, this study investigated the antihypertensive and vascular protective effects of L. rhinocerus TM02 (R) sclerotia supplementation in spontaneously hypertensive rats (SHR). Wistar Kyoto (WKY) rats served as a normo-tensive control group. SHR were orally administered with L. rhinocerus TM02 (R) sclerotia (100 mg/kg and 300 mg/kg, respectively) for 8 weeks, and blood pressure was monitored every 2 weeks. Vascular function was evaluated using an organ bath (aorta) and wire myograph (renal artery) at the treatment endpoint. The levels of reactive oxygen species (ROS) and nitric oxide (NO) in the aorta and renal artery were evaluated using dihydroethidium (DHE) and difluoro fluorescein acetate (DAF-FM) fluorescence assays, respectively. Total plasma nitrate/nitrite and tumor necrosis factor alpha (TNF-alpha) levels were evaluated via colorimetric assays. In vivo treatment with L. rhinocerus TM02 (R) sclerotia sig-nificantly attenuated the increase in systolic blood pressure (SBP). It also alleviated vascular dysfunction and decreased elevated ROS in the aorta and renal arteries of the treated SHRs. Moreover, L. rhinocerus TM02 (R) sclerotia attenuated plasma TNF-alpha level but increased total plasma nitrate/nitrite, albeit slightly, coupled with significantly increased NO at the vascular level. Collectively, the present study demonstrated that L. rhinocerus TM02 (R) sclerotia supplementation exerted blood pressure lowering effects, partly attributed to improvements in vascular function via reduction in vascular oxidative stress.
format	Article
author	Wong, Yi En Razif, Muhammad Fazril Mohamad Ng, Szu-Ting Tan, Chon-Seng Fung, Shin-Yee Murugan, Dharmani Devi
author_facet	Wong, Yi En Razif, Muhammad Fazril Mohamad Ng, Szu-Ting Tan, Chon-Seng Fung, Shin-Yee Murugan, Dharmani Devi
author_sort	Wong, Yi En
title	Medicinal Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) Sclerotia Supplementation Mitigates Hypertension and Alleviates Vascular Dysfunction Partly through Oxidative Stress Modulation in Spontaneously Hypertensive Rats
title_short	Medicinal Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) Sclerotia Supplementation Mitigates Hypertension and Alleviates Vascular Dysfunction Partly through Oxidative Stress Modulation in Spontaneously Hypertensive Rats
title_full	Medicinal Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) Sclerotia Supplementation Mitigates Hypertension and Alleviates Vascular Dysfunction Partly through Oxidative Stress Modulation in Spontaneously Hypertensive Rats
title_fullStr	Medicinal Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) Sclerotia Supplementation Mitigates Hypertension and Alleviates Vascular Dysfunction Partly through Oxidative Stress Modulation in Spontaneously Hypertensive Rats
title_full_unstemmed	Medicinal Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) Sclerotia Supplementation Mitigates Hypertension and Alleviates Vascular Dysfunction Partly through Oxidative Stress Modulation in Spontaneously Hypertensive Rats
title_sort	medicinal tiger milk mushroom lignosus rhinocerus tm02® (agaricomycetes) sclerotia supplementation mitigates hypertension and alleviates vascular dysfunction partly through oxidative stress modulation in spontaneously hypertensive rats
publisher	Begell House
publishDate	2024
url	http://eprints.um.edu.my/47125/ https://doi.org/10.1615/IntJMedMushrooms.2024055061
_version_	1817841984314802176
score	13.223943

Medicinal Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) Sclerotia Supplementation Mitigates Hypertension and Alleviates Vascular Dysfunction Partly through Oxidative Stress Modulation in Spontaneously Hypertensive Rats

Similar Items