Staff View: Distribution of virulence genes in clinical isolates of hospital-associated and community-associated methicillin-susceptible Staphylococcus aureus from Terengganu, Malaysia

Distribution of virulence genes in clinical isolates of hospital-associated and community-associated methicillin-susceptible Staphylococcus aureus from Terengganu, Malaysia

Staphylococcus aureus is a common bacterial pathogen known to cause various kinds of infections due to its repertoire of virulence factors. This study aimed to investigate the distribution of 19 types of virulence genes among clinical isolates of methicillin-susceptible S. aureus (MSSA) using the po...

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Main Authors:	Hamzah, A. M. Che, Yeo, C. C., Puah, S. M., Chua, K. H., Rahman, N., Ismail, S., Abdullah, F. H., Othman, N., Chew, C. H.
Format:	Article
Published:	Malaysian Society of Parasitology and Tropical Medicine 2024
Subjects:	R Medicine (General)
Online Access:	http://eprints.um.edu.my/47086/ https://doi.org/10.47665/tb.41.2.003
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spelling	my.um.eprints.470862024-11-22T01:26:10Z http://eprints.um.edu.my/47086/ Distribution of virulence genes in clinical isolates of hospital-associated and community-associated methicillin-susceptible Staphylococcus aureus from Terengganu, Malaysia Hamzah, A. M. Che Yeo, C. C. Puah, S. M. Chua, K. H. Rahman, N. Ismail, S. Abdullah, F. H. Othman, N. Chew, C. H. R Medicine (General) Staphylococcus aureus is a common bacterial pathogen known to cause various kinds of infections due to its repertoire of virulence factors. This study aimed to investigate the distribution of 19 types of virulence genes among clinical isolates of methicillin-susceptible S. aureus (MSSA) using the polymerase chain reaction. A total of 109 MSSA isolates, i.e., 63 hospital-associated (HA) and 46 community-associated (CA) were collected from Hospital Sultanah Nur Zahirah, the main tertiary hospital in Terengganu, Malaysia, from July 2016 to June 2017. The most frequent virulence genes detected were hla (78.9%, n =86) and hld (78.0%, n =85) encoding hemolysins, lukED (56.9%, n =62) encoding leukotoxin ED, followed by seb (26.6%, n =29) and sea (24.8%, n =27) encoding enterotoxins. Among 34 (31.2%) isolates carrying six or more virulence genes, only five were multidrug resistant (MDR) while the remaining isolates were susceptible. Significant associations were discovered between the hld gene with CA-MSSA (p=0.016) p =0.016) and the seo gene with HA-MSSA (p=0.023). p =0.023). However, there is no significant association between virulence genes among the different types of infection. The clinical MSSA isolates in Terengganu showed high prevalence and high diversity of virulence gene carriage. Malaysian Society of Parasitology and Tropical Medicine 2024 Article PeerReviewed Hamzah, A. M. Che and Yeo, C. C. and Puah, S. M. and Chua, K. H. and Rahman, N. and Ismail, S. and Abdullah, F. H. and Othman, N. and Chew, C. H. (2024) Distribution of virulence genes in clinical isolates of hospital-associated and community-associated methicillin-susceptible Staphylococcus aureus from Terengganu, Malaysia. Tropical Biomedicine, 41 (2). pp. 149-156. ISSN 0127-5720, DOI https://doi.org/10.47665/tb.41.2.003 <https://doi.org/10.47665/tb.41.2.003>. https://doi.org/10.47665/tb.41.2.003 10.47665/tb.41.2.003
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	R Medicine (General)
spellingShingle	R Medicine (General) Hamzah, A. M. Che Yeo, C. C. Puah, S. M. Chua, K. H. Rahman, N. Ismail, S. Abdullah, F. H. Othman, N. Chew, C. H. Distribution of virulence genes in clinical isolates of hospital-associated and community-associated methicillin-susceptible Staphylococcus aureus from Terengganu, Malaysia
description	Staphylococcus aureus is a common bacterial pathogen known to cause various kinds of infections due to its repertoire of virulence factors. This study aimed to investigate the distribution of 19 types of virulence genes among clinical isolates of methicillin-susceptible S. aureus (MSSA) using the polymerase chain reaction. A total of 109 MSSA isolates, i.e., 63 hospital-associated (HA) and 46 community-associated (CA) were collected from Hospital Sultanah Nur Zahirah, the main tertiary hospital in Terengganu, Malaysia, from July 2016 to June 2017. The most frequent virulence genes detected were hla (78.9%, n =86) and hld (78.0%, n =85) encoding hemolysins, lukED (56.9%, n =62) encoding leukotoxin ED, followed by seb (26.6%, n =29) and sea (24.8%, n =27) encoding enterotoxins. Among 34 (31.2%) isolates carrying six or more virulence genes, only five were multidrug resistant (MDR) while the remaining isolates were susceptible. Significant associations were discovered between the hld gene with CA-MSSA (p=0.016) p =0.016) and the seo gene with HA-MSSA (p=0.023). p =0.023). However, there is no significant association between virulence genes among the different types of infection. The clinical MSSA isolates in Terengganu showed high prevalence and high diversity of virulence gene carriage.
format	Article
author	Hamzah, A. M. Che Yeo, C. C. Puah, S. M. Chua, K. H. Rahman, N. Ismail, S. Abdullah, F. H. Othman, N. Chew, C. H.
author_facet	Hamzah, A. M. Che Yeo, C. C. Puah, S. M. Chua, K. H. Rahman, N. Ismail, S. Abdullah, F. H. Othman, N. Chew, C. H.
author_sort	Hamzah, A. M. Che
title	Distribution of virulence genes in clinical isolates of hospital-associated and community-associated methicillin-susceptible Staphylococcus aureus from Terengganu, Malaysia
title_short	Distribution of virulence genes in clinical isolates of hospital-associated and community-associated methicillin-susceptible Staphylococcus aureus from Terengganu, Malaysia
title_full	Distribution of virulence genes in clinical isolates of hospital-associated and community-associated methicillin-susceptible Staphylococcus aureus from Terengganu, Malaysia
title_fullStr	Distribution of virulence genes in clinical isolates of hospital-associated and community-associated methicillin-susceptible Staphylococcus aureus from Terengganu, Malaysia
title_full_unstemmed	Distribution of virulence genes in clinical isolates of hospital-associated and community-associated methicillin-susceptible Staphylococcus aureus from Terengganu, Malaysia
title_sort	distribution of virulence genes in clinical isolates of hospital-associated and community-associated methicillin-susceptible staphylococcus aureus from terengganu, malaysia
publisher	Malaysian Society of Parasitology and Tropical Medicine
publishDate	2024
url	http://eprints.um.edu.my/47086/ https://doi.org/10.47665/tb.41.2.003
_version_	1817841973306851328
score	13.244413

Distribution of virulence genes in clinical isolates of hospital-associated and community-associated methicillin-susceptible Staphylococcus aureus from Terengganu, Malaysia

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