Proteomic Analysis of Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles: A Systematic Review
Numerous challenges remain within conventional cell-based therapy despite the growing trend of stem cells used to treat various life-debilitating diseases. These limitations include batch-to-batch heterogeneity, induced alloreactivity, cell survival and integration, poor scalability, and high cost o...
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my.um.eprints.469592025-01-13T01:17:12Z http://eprints.um.edu.my/46959/ Proteomic Analysis of Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles: A Systematic Review Krishnan, Illayaraja Chan, Alvin Man Lung Law, Jia Xian Ng, Min Hwei Jayapalan, Jaime Jacqueline Lokanathan, Yogeswaran R Medicine (General) Numerous challenges remain within conventional cell-based therapy despite the growing trend of stem cells used to treat various life-debilitating diseases. These limitations include batch-to-batch heterogeneity, induced alloreactivity, cell survival and integration, poor scalability, and high cost of treatment, thus hindering successful translation from lab to bedside. However, recent pioneering technology has enabled the isolation and enrichment of small extracellular vesicles (EVs), canonically known as exosomes. EVs are described as a membrane-enclosed cargo of functional biomolecules not limited to lipids, nucleic acid, and proteins. Interestingly, studies have correlated the biological role of MSC-EVs to the paracrine activity of MSCs. This key evidence has led to rigorous studies on MSC-EVs as an acellular alternative. Using EVs as a therapy was proposed as a model leading to improvements through increased safety; enhanced bioavailability due to size and permeability; reduced heterogeneity by selective and quantifiable properties; and prolonged shelf-life via long-term freezing or lyophilization. Yet, the identity and potency of EVs are still relatively unknown due to various methods of preparation and to qualify the final product. This is reflected by the absence of regulatory strategies overseeing manufacturing, quality control, clinical implementation, and product registration. In this review, the authors review the various production processes and the proteomic profile of MSC-EVs. MDPI 2024-05 Article PeerReviewed Krishnan, Illayaraja and Chan, Alvin Man Lung and Law, Jia Xian and Ng, Min Hwei and Jayapalan, Jaime Jacqueline and Lokanathan, Yogeswaran (2024) Proteomic Analysis of Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles: A Systematic Review. International Journal of Molecular Sciences, 25 (10). p. 5340. ISSN 1661-6596, DOI https://doi.org/10.3390/ijms25105340 <https://doi.org/10.3390/ijms25105340>. https://doi.org/10.3390/ijms25105340 10.3390/ijms25105340 |
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R Medicine (General) Krishnan, Illayaraja Chan, Alvin Man Lung Law, Jia Xian Ng, Min Hwei Jayapalan, Jaime Jacqueline Lokanathan, Yogeswaran Proteomic Analysis of Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles: A Systematic Review |
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Numerous challenges remain within conventional cell-based therapy despite the growing trend of stem cells used to treat various life-debilitating diseases. These limitations include batch-to-batch heterogeneity, induced alloreactivity, cell survival and integration, poor scalability, and high cost of treatment, thus hindering successful translation from lab to bedside. However, recent pioneering technology has enabled the isolation and enrichment of small extracellular vesicles (EVs), canonically known as exosomes. EVs are described as a membrane-enclosed cargo of functional biomolecules not limited to lipids, nucleic acid, and proteins. Interestingly, studies have correlated the biological role of MSC-EVs to the paracrine activity of MSCs. This key evidence has led to rigorous studies on MSC-EVs as an acellular alternative. Using EVs as a therapy was proposed as a model leading to improvements through increased safety; enhanced bioavailability due to size and permeability; reduced heterogeneity by selective and quantifiable properties; and prolonged shelf-life via long-term freezing or lyophilization. Yet, the identity and potency of EVs are still relatively unknown due to various methods of preparation and to qualify the final product. This is reflected by the absence of regulatory strategies overseeing manufacturing, quality control, clinical implementation, and product registration. In this review, the authors review the various production processes and the proteomic profile of MSC-EVs. |
format |
Article |
author |
Krishnan, Illayaraja Chan, Alvin Man Lung Law, Jia Xian Ng, Min Hwei Jayapalan, Jaime Jacqueline Lokanathan, Yogeswaran |
author_facet |
Krishnan, Illayaraja Chan, Alvin Man Lung Law, Jia Xian Ng, Min Hwei Jayapalan, Jaime Jacqueline Lokanathan, Yogeswaran |
author_sort |
Krishnan, Illayaraja |
title |
Proteomic Analysis of Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles: A Systematic Review |
title_short |
Proteomic Analysis of Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles: A Systematic Review |
title_full |
Proteomic Analysis of Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles: A Systematic Review |
title_fullStr |
Proteomic Analysis of Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles: A Systematic Review |
title_full_unstemmed |
Proteomic Analysis of Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles: A Systematic Review |
title_sort |
proteomic analysis of umbilical cord mesenchymal stem cell-derived extracellular vesicles: a systematic review |
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MDPI |
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2024 |
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http://eprints.um.edu.my/46959/ https://doi.org/10.3390/ijms25105340 |
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1821105744912580608 |
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13.226497 |