Staff View: The Effects of Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) on Leukemogenicity Tyrosine Kinase Cell Lines

The Effects of Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) on Leukemogenicity Tyrosine Kinase Cell Lines

Leukemia can be a result of genetic changes associated with protein tyrosine kinase activity such as in MPL W515L and BCR/ABL genes. However, the current conventional treatment of leukemia produces severe side effects that urge the approach to use natural products. A medicinal mushroom, Lignosus rhi...

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Main Authors:	Yahya, Tuan Syaripah Atiqah Najwa Tuan, Azmi, Norhaida Che, Yee, Fung Shin, Chyang, Pang Jyh, Ting, Ng Szu, Seng, Tan Chon
Format:	Article
Published:	Begell House 2024
Subjects:	R Medicine (General) RM Therapeutics. Pharmacology RS Pharmacy and materia medica
Online Access:	http://eprints.um.edu.my/45907/ https://www.dl.begellhouse.com/journals/708ae68d64b17c52,30f8ca037b23320e,155c51303a26442d.html
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spelling	my.um.eprints.459072024-11-14T04:04:51Z http://eprints.um.edu.my/45907/ The Effects of Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) on Leukemogenicity Tyrosine Kinase Cell Lines Yahya, Tuan Syaripah Atiqah Najwa Tuan Azmi, Norhaida Che Yee, Fung Shin Chyang, Pang Jyh Ting, Ng Szu Seng, Tan Chon R Medicine (General) RM Therapeutics. Pharmacology RS Pharmacy and materia medica Leukemia can be a result of genetic changes associated with protein tyrosine kinase activity such as in MPL W515L and BCR/ABL genes. However, the current conventional treatment of leukemia produces severe side effects that urge the approach to use natural products. A medicinal mushroom, Lignosus rhinocerus shows potential as an anti-cancer treatment. To investigate the effi cacy and mechanism of action of the L. rhinocerus cultivar (TM02 (R)) extract on leukemogenic tyrosine kinase cell lines, a cold-water extract (CWE) was produced by using TM02 (R) sclerotia powder at 4 degrees C. The carbohydrate and protein contents were found to be 77.24% and 1.75% respectively. In comparison to the normal Ba/F3 cell, the CWE TM02 (R) shows significant effects on exhibiting proliferation of Ba/F3 expressed MPL W515L and BCR/ABL, possibly due to the presence of phenolic compounds and antioxidant properties of TM02 (R), which contribute to act on various signaling pathways, and the reported apoptotic activity of CWE TM02 (R). In contrast, CWE TM02 (R) significantly exhibited high scavenging activity of both Ba/F3 expressed MPL W515L and BCR/ABL. At concentrations of 125 mu g/mL and 500 mu g/mL of CWE TM02 (R) decreased 49.5% and 67.5% of cell migration activity of Ba/F3 expressed MPL W515L and BCR/ABL respectively. Therefore, we postulate that CWE TM02 (R) has the capability to mediate the migration route of the leukemogenic tyrosine kinase cell lines. Begell House 2024 Article PeerReviewed Yahya, Tuan Syaripah Atiqah Najwa Tuan and Azmi, Norhaida Che and Yee, Fung Shin and Chyang, Pang Jyh and Ting, Ng Szu and Seng, Tan Chon (2024) The Effects of Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) on Leukemogenicity Tyrosine Kinase Cell Lines. International Journal of Medicinal Mushrooms, 26 (3). pp. 55-66. ISSN 1521-9437, https://www.dl.begellhouse.com/journals/708ae68d64b17c52,30f8ca037b23320e,155c51303a26442d.html
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	R Medicine (General) RM Therapeutics. Pharmacology RS Pharmacy and materia medica
spellingShingle	R Medicine (General) RM Therapeutics. Pharmacology RS Pharmacy and materia medica Yahya, Tuan Syaripah Atiqah Najwa Tuan Azmi, Norhaida Che Yee, Fung Shin Chyang, Pang Jyh Ting, Ng Szu Seng, Tan Chon The Effects of Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) on Leukemogenicity Tyrosine Kinase Cell Lines
description	Leukemia can be a result of genetic changes associated with protein tyrosine kinase activity such as in MPL W515L and BCR/ABL genes. However, the current conventional treatment of leukemia produces severe side effects that urge the approach to use natural products. A medicinal mushroom, Lignosus rhinocerus shows potential as an anti-cancer treatment. To investigate the effi cacy and mechanism of action of the L. rhinocerus cultivar (TM02 (R)) extract on leukemogenic tyrosine kinase cell lines, a cold-water extract (CWE) was produced by using TM02 (R) sclerotia powder at 4 degrees C. The carbohydrate and protein contents were found to be 77.24% and 1.75% respectively. In comparison to the normal Ba/F3 cell, the CWE TM02 (R) shows significant effects on exhibiting proliferation of Ba/F3 expressed MPL W515L and BCR/ABL, possibly due to the presence of phenolic compounds and antioxidant properties of TM02 (R), which contribute to act on various signaling pathways, and the reported apoptotic activity of CWE TM02 (R). In contrast, CWE TM02 (R) significantly exhibited high scavenging activity of both Ba/F3 expressed MPL W515L and BCR/ABL. At concentrations of 125 mu g/mL and 500 mu g/mL of CWE TM02 (R) decreased 49.5% and 67.5% of cell migration activity of Ba/F3 expressed MPL W515L and BCR/ABL respectively. Therefore, we postulate that CWE TM02 (R) has the capability to mediate the migration route of the leukemogenic tyrosine kinase cell lines.
format	Article
author	Yahya, Tuan Syaripah Atiqah Najwa Tuan Azmi, Norhaida Che Yee, Fung Shin Chyang, Pang Jyh Ting, Ng Szu Seng, Tan Chon
author_facet	Yahya, Tuan Syaripah Atiqah Najwa Tuan Azmi, Norhaida Che Yee, Fung Shin Chyang, Pang Jyh Ting, Ng Szu Seng, Tan Chon
author_sort	Yahya, Tuan Syaripah Atiqah Najwa Tuan
title	The Effects of Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) on Leukemogenicity Tyrosine Kinase Cell Lines
title_short	The Effects of Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) on Leukemogenicity Tyrosine Kinase Cell Lines
title_full	The Effects of Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) on Leukemogenicity Tyrosine Kinase Cell Lines
title_fullStr	The Effects of Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) on Leukemogenicity Tyrosine Kinase Cell Lines
title_full_unstemmed	The Effects of Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) on Leukemogenicity Tyrosine Kinase Cell Lines
title_sort	effects of tiger milk mushroom lignosus rhinocerus tm02® (agaricomycetes) on leukemogenicity tyrosine kinase cell lines
publisher	Begell House
publishDate	2024
url	http://eprints.um.edu.my/45907/ https://www.dl.begellhouse.com/journals/708ae68d64b17c52,30f8ca037b23320e,155c51303a26442d.html
_version_	1816130476013780992
score	13.223943

The Effects of Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) on Leukemogenicity Tyrosine Kinase Cell Lines

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