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Role of cell surface proteins and toll-like receptors in the pathogenesis of Streptococcus pneumoniae

Objective(s): Pneumococcal cell wall (PCW) is an inflammatory component in Streptococcus pneumoniae. The cell surface proteins and the toll-like receptors (TLR) signaling response were investigated in the human lung epithelial (A549) cells inoculated with PCW of different serotypes.Materials and Met...

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Main Authors: Anuar, Nurul Fathiyah Zaipul, Desa, Mohd Nasir Mohd, Hussaini, Jamal, Wong, Eng Hwa, Mariappan, Vanitha, Vellasamy, Kumutha Malar, Palanisamy, Navindra Kumari
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Published: Mashhad University of Medical Sciences 2024
Subjects:
R Medicine (General)
Online Access:http://eprints.um.edu.my/45672/
https://doi.org/10.22038/IJBMS.2023.72584.15791
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spelling my.um.eprints.456722024-11-08T04:08:17Z http://eprints.um.edu.my/45672/ Role of cell surface proteins and toll-like receptors in the pathogenesis of Streptococcus pneumoniae Anuar, Nurul Fathiyah Zaipul Desa, Mohd Nasir Mohd Hussaini, Jamal Wong, Eng Hwa Mariappan, Vanitha Vellasamy, Kumutha Malar Palanisamy, Navindra Kumari R Medicine (General) Objective(s): Pneumococcal cell wall (PCW) is an inflammatory component in Streptococcus pneumoniae. The cell surface proteins and the toll-like receptors (TLR) signaling response were investigated in the human lung epithelial (A549) cells inoculated with PCW of different serotypes.Materials and Methods: The presence of genes encoding these proteins was determined using polymerase chain reaction (PCR). The structure of the cell walls was analyzed by proton nuclear magnetic resonance (1H NMR). The A549 cell line was challenged with PCW extracts of different serotypes. RNA from the infected host cells was extracted and tested against a total of 84 genes associated with TLR signaling pathways (TLR 1-6 and 10) using RT2 Profiler PCR Array. Results: Cell surface proteins; ply, lytA, nanA, nanB, and cbpD genes were present in all serotypes. The distribution and structure of surface protein genes suggest behavioral changes in the molecules, contributing to the resilience of the strains to antibiotic treatment. Conclusion: TLR2 showed the highest expression, while serotypes 1, 3, and 5 induced higher TNF alpha and IL-1 alpha, suggesting to be more immunogenic than the other strains tested. Mashhad University of Medical Sciences 2024-02 Article PeerReviewed Anuar, Nurul Fathiyah Zaipul and Desa, Mohd Nasir Mohd and Hussaini, Jamal and Wong, Eng Hwa and Mariappan, Vanitha and Vellasamy, Kumutha Malar and Palanisamy, Navindra Kumari (2024) Role of cell surface proteins and toll-like receptors in the pathogenesis of Streptococcus pneumoniae. Iranian Journal of Basic Medical Sciences, 27 (2). pp. 214-222. ISSN 2008-3866, DOI https://doi.org/10.22038/IJBMS.2023.72584.15791 <https://doi.org/10.22038/IJBMS.2023.72584.15791>. https://doi.org/10.22038/IJBMS.2023.72584.15791 10.22038/IJBMS.2023.72584.15791
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine (General)
spellingShingle R Medicine (General)
Anuar, Nurul Fathiyah Zaipul
Desa, Mohd Nasir Mohd
Hussaini, Jamal
Wong, Eng Hwa
Mariappan, Vanitha
Vellasamy, Kumutha Malar
Palanisamy, Navindra Kumari
Role of cell surface proteins and toll-like receptors in the pathogenesis of Streptococcus pneumoniae
description Objective(s): Pneumococcal cell wall (PCW) is an inflammatory component in Streptococcus pneumoniae. The cell surface proteins and the toll-like receptors (TLR) signaling response were investigated in the human lung epithelial (A549) cells inoculated with PCW of different serotypes.Materials and Methods: The presence of genes encoding these proteins was determined using polymerase chain reaction (PCR). The structure of the cell walls was analyzed by proton nuclear magnetic resonance (1H NMR). The A549 cell line was challenged with PCW extracts of different serotypes. RNA from the infected host cells was extracted and tested against a total of 84 genes associated with TLR signaling pathways (TLR 1-6 and 10) using RT2 Profiler PCR Array. Results: Cell surface proteins; ply, lytA, nanA, nanB, and cbpD genes were present in all serotypes. The distribution and structure of surface protein genes suggest behavioral changes in the molecules, contributing to the resilience of the strains to antibiotic treatment. Conclusion: TLR2 showed the highest expression, while serotypes 1, 3, and 5 induced higher TNF alpha and IL-1 alpha, suggesting to be more immunogenic than the other strains tested.
format Article
author Anuar, Nurul Fathiyah Zaipul
Desa, Mohd Nasir Mohd
Hussaini, Jamal
Wong, Eng Hwa
Mariappan, Vanitha
Vellasamy, Kumutha Malar
Palanisamy, Navindra Kumari
author_facet Anuar, Nurul Fathiyah Zaipul
Desa, Mohd Nasir Mohd
Hussaini, Jamal
Wong, Eng Hwa
Mariappan, Vanitha
Vellasamy, Kumutha Malar
Palanisamy, Navindra Kumari
author_sort Anuar, Nurul Fathiyah Zaipul
title Role of cell surface proteins and toll-like receptors in the pathogenesis of Streptococcus pneumoniae
title_short Role of cell surface proteins and toll-like receptors in the pathogenesis of Streptococcus pneumoniae
title_full Role of cell surface proteins and toll-like receptors in the pathogenesis of Streptococcus pneumoniae
title_fullStr Role of cell surface proteins and toll-like receptors in the pathogenesis of Streptococcus pneumoniae
title_full_unstemmed Role of cell surface proteins and toll-like receptors in the pathogenesis of Streptococcus pneumoniae
title_sort role of cell surface proteins and toll-like receptors in the pathogenesis of streptococcus pneumoniae
publisher Mashhad University of Medical Sciences
publishDate 2024
url http://eprints.um.edu.my/45672/
https://doi.org/10.22038/IJBMS.2023.72584.15791
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score 13.223943

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