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Sciscionella sediminilitoris sp. nov., a Marine Actinomycete Isolated from Cape Rochado, Malaysia, and the Emendations to the Description of the Genus Sciscionella

In this study, we employed a polyphasic approach to determine the taxonomic position of a newly isolated actinomycete, designated SE31T, obtained from a sediment sample collected at Cape Rochado, Malaysia. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain SE31T belonged to the...

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Main Authors: Teo, Wee Fei Aaron, Devaraj, Kavimalar, Nor, Muhammad Nuruddin Mohd, Li, Wen-Jun, Tan, Geok Yuan Annie
Format: Article
Published: Springer Verlag (Germany) 2024
Subjects:
Q Science (General)
QR Microbiology
Online Access:http://eprints.um.edu.my/45278/
https://doi.org/10.1007/s00284-024-03634-8
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spelling my.um.eprints.452782024-09-30T08:14:50Z http://eprints.um.edu.my/45278/ Sciscionella sediminilitoris sp. nov., a Marine Actinomycete Isolated from Cape Rochado, Malaysia, and the Emendations to the Description of the Genus Sciscionella Teo, Wee Fei Aaron Devaraj, Kavimalar Nor, Muhammad Nuruddin Mohd Li, Wen-Jun Tan, Geok Yuan Annie Q Science (General) QR Microbiology In this study, we employed a polyphasic approach to determine the taxonomic position of a newly isolated actinomycete, designated SE31T, obtained from a sediment sample collected at Cape Rochado, Malaysia. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain SE31T belonged to the family Pseudonocardiaceae and exhibited the highest sequence similarity (98.9%) to Sciscionella marina. Further genomic analysis demonstrated a 93.4% average nucleotide identity and 54.4% digital DNA-DNA hybridization relatedness between strain SE31T and S. marina. The chemotaxonomic characteristics of strain SE31T were typical of the genus Sciscionella, including cell-wall chemotype IV (with meso-diaminopimelic acid as the diagnostic diamino acid, and arabinose and galactose as whole-cell sugars). The identified polar lipids of strain SE31T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, and hydroxyphosphatidymethylethanolamine. The primary menaquinone observed was MK-9(H4), and the major cellular fatty acid was iso-C16:0. The genomic DNA size of strain SE31T was determined to be 7.4 Mbp with a G+C content of 68.7%. Based on these comprehensive findings, strain SE31T represents a novel species within the genus Sciscionella, in which the name Sciscionella sediminilitoris sp. nov. is proposed. The type strain of Sciscionella sediminilitoris is SE31T (= DSM 46824T = TBRC 5134T). Springer Verlag (Germany) 2024-05 Article PeerReviewed Teo, Wee Fei Aaron and Devaraj, Kavimalar and Nor, Muhammad Nuruddin Mohd and Li, Wen-Jun and Tan, Geok Yuan Annie (2024) Sciscionella sediminilitoris sp. nov., a Marine Actinomycete Isolated from Cape Rochado, Malaysia, and the Emendations to the Description of the Genus Sciscionella. Current Microbiology, 81 (5). p. 124. ISSN 0343-8651, DOI https://doi.org/10.1007/s00284-024-03634-8 <https://doi.org/10.1007/s00284-024-03634-8>. https://doi.org/10.1007/s00284-024-03634-8 10.1007/s00284-024-03634-8
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic Q Science (General)
QR Microbiology
spellingShingle Q Science (General)
QR Microbiology
Teo, Wee Fei Aaron
Devaraj, Kavimalar
Nor, Muhammad Nuruddin Mohd
Li, Wen-Jun
Tan, Geok Yuan Annie
Sciscionella sediminilitoris sp. nov., a Marine Actinomycete Isolated from Cape Rochado, Malaysia, and the Emendations to the Description of the Genus Sciscionella
description In this study, we employed a polyphasic approach to determine the taxonomic position of a newly isolated actinomycete, designated SE31T, obtained from a sediment sample collected at Cape Rochado, Malaysia. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain SE31T belonged to the family Pseudonocardiaceae and exhibited the highest sequence similarity (98.9%) to Sciscionella marina. Further genomic analysis demonstrated a 93.4% average nucleotide identity and 54.4% digital DNA-DNA hybridization relatedness between strain SE31T and S. marina. The chemotaxonomic characteristics of strain SE31T were typical of the genus Sciscionella, including cell-wall chemotype IV (with meso-diaminopimelic acid as the diagnostic diamino acid, and arabinose and galactose as whole-cell sugars). The identified polar lipids of strain SE31T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, and hydroxyphosphatidymethylethanolamine. The primary menaquinone observed was MK-9(H4), and the major cellular fatty acid was iso-C16:0. The genomic DNA size of strain SE31T was determined to be 7.4 Mbp with a G+C content of 68.7%. Based on these comprehensive findings, strain SE31T represents a novel species within the genus Sciscionella, in which the name Sciscionella sediminilitoris sp. nov. is proposed. The type strain of Sciscionella sediminilitoris is SE31T (= DSM 46824T = TBRC 5134T).
format Article
author Teo, Wee Fei Aaron
Devaraj, Kavimalar
Nor, Muhammad Nuruddin Mohd
Li, Wen-Jun
Tan, Geok Yuan Annie
author_facet Teo, Wee Fei Aaron
Devaraj, Kavimalar
Nor, Muhammad Nuruddin Mohd
Li, Wen-Jun
Tan, Geok Yuan Annie
author_sort Teo, Wee Fei Aaron
title Sciscionella sediminilitoris sp. nov., a Marine Actinomycete Isolated from Cape Rochado, Malaysia, and the Emendations to the Description of the Genus Sciscionella
title_short Sciscionella sediminilitoris sp. nov., a Marine Actinomycete Isolated from Cape Rochado, Malaysia, and the Emendations to the Description of the Genus Sciscionella
title_full Sciscionella sediminilitoris sp. nov., a Marine Actinomycete Isolated from Cape Rochado, Malaysia, and the Emendations to the Description of the Genus Sciscionella
title_fullStr Sciscionella sediminilitoris sp. nov., a Marine Actinomycete Isolated from Cape Rochado, Malaysia, and the Emendations to the Description of the Genus Sciscionella
title_full_unstemmed Sciscionella sediminilitoris sp. nov., a Marine Actinomycete Isolated from Cape Rochado, Malaysia, and the Emendations to the Description of the Genus Sciscionella
title_sort sciscionella sediminilitoris sp. nov., a marine actinomycete isolated from cape rochado, malaysia, and the emendations to the description of the genus sciscionella
publisher Springer Verlag (Germany)
publishDate 2024
url http://eprints.um.edu.my/45278/
https://doi.org/10.1007/s00284-024-03634-8
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score 13.211869

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