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In-tip solid-phase microextraction: a method for determination of sulphonamide residues in environmental water samples

This study involved the development of an in-pipette tip solid-phase microextraction (SPME) method using activated charcoal as an adsorbent. Simultaneous determination of three sulphonamide antibiotic residues in environmental water was performed using this method, coupled with high-performance liqu...

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Main Authors: Lu, Tan Lei, Shahriman, Mohamad Shariff, Sambasevam, Kavirajaa Pandian, Zain, Nur Nadhirah Mohamad, Yahaya, Noorfatimah, Lim, Vuanghao, Raoov, Muggundha
Format: Article
Published: Taylor & Francis 2024
Subjects:
QD Chemistry
S Agriculture (General)
Online Access:http://eprints.um.edu.my/44340/
https://doi.org/10.1080/03067319.2021.2019721
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spelling my.um.eprints.443402024-07-09T04:18:30Z http://eprints.um.edu.my/44340/ In-tip solid-phase microextraction: a method for determination of sulphonamide residues in environmental water samples Lu, Tan Lei Shahriman, Mohamad Shariff Sambasevam, Kavirajaa Pandian Zain, Nur Nadhirah Mohamad Yahaya, Noorfatimah Lim, Vuanghao Raoov, Muggundha QD Chemistry S Agriculture (General) This study involved the development of an in-pipette tip solid-phase microextraction (SPME) method using activated charcoal as an adsorbent. Simultaneous determination of three sulphonamide antibiotic residues in environmental water was performed using this method, coupled with high-performance liquid chromatography-photodiode array (HPLC-PDA) detection. Seven extraction parameters were optimised by one-variable-at-a-time (OVAT) and response surface (RSM) methods. Optimum extraction efficiency was achieved when 10 mL of sample solution at a natural pH (pH 5-6) was loaded through a 1 mL pipette tip packed with 10 mg of adsorbent, washed with 1 mL of hexane and eluted with 500 mu L of 1% ammonium in methanol solution. Under the optimised experimental condition, this method manifested good linearity (5-500 mu g L-1) at the coefficient of determination, R-2 of 0.9992 to 0.9993, high sensitivity (limit of detection, LOD: 0.38-1.14 mu g L-1; limit of quantification, LOQ: 1.14-3.35 mu g L-1), and satisfactory recoveries (82.8-108.7%) with acceptable reproducibility (RSD < 4.6%). Real sample analysis of five environmental water samples sourced from Universiti Malaya, Kuala Lumpur and Malacca showed that the samples contained either none or traces of the target sulphonamides (SAs) with their concentration lower than the LOQs. In contrast to other methods, the comparison outcomes depicted that this in-tip SPME method was beneficial in terms of its simple, reusable, cost-saving setup together with a significant reduction in chemical consumption. Taylor & Francis 2024-01-26 Article PeerReviewed Lu, Tan Lei and Shahriman, Mohamad Shariff and Sambasevam, Kavirajaa Pandian and Zain, Nur Nadhirah Mohamad and Yahaya, Noorfatimah and Lim, Vuanghao and Raoov, Muggundha (2024) In-tip solid-phase microextraction: a method for determination of sulphonamide residues in environmental water samples. International Journal of Environmental Analytical Chemistry, 104 (2). pp. 261-276. ISSN 0306-7319, DOI https://doi.org/10.1080/03067319.2021.2019721 <https://doi.org/10.1080/03067319.2021.2019721>. https://doi.org/10.1080/03067319.2021.2019721 10.1080/03067319.2021.2019721
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QD Chemistry
S Agriculture (General)
spellingShingle QD Chemistry
S Agriculture (General)
Lu, Tan Lei
Shahriman, Mohamad Shariff
Sambasevam, Kavirajaa Pandian
Zain, Nur Nadhirah Mohamad
Yahaya, Noorfatimah
Lim, Vuanghao
Raoov, Muggundha
In-tip solid-phase microextraction: a method for determination of sulphonamide residues in environmental water samples
description This study involved the development of an in-pipette tip solid-phase microextraction (SPME) method using activated charcoal as an adsorbent. Simultaneous determination of three sulphonamide antibiotic residues in environmental water was performed using this method, coupled with high-performance liquid chromatography-photodiode array (HPLC-PDA) detection. Seven extraction parameters were optimised by one-variable-at-a-time (OVAT) and response surface (RSM) methods. Optimum extraction efficiency was achieved when 10 mL of sample solution at a natural pH (pH 5-6) was loaded through a 1 mL pipette tip packed with 10 mg of adsorbent, washed with 1 mL of hexane and eluted with 500 mu L of 1% ammonium in methanol solution. Under the optimised experimental condition, this method manifested good linearity (5-500 mu g L-1) at the coefficient of determination, R-2 of 0.9992 to 0.9993, high sensitivity (limit of detection, LOD: 0.38-1.14 mu g L-1; limit of quantification, LOQ: 1.14-3.35 mu g L-1), and satisfactory recoveries (82.8-108.7%) with acceptable reproducibility (RSD < 4.6%). Real sample analysis of five environmental water samples sourced from Universiti Malaya, Kuala Lumpur and Malacca showed that the samples contained either none or traces of the target sulphonamides (SAs) with their concentration lower than the LOQs. In contrast to other methods, the comparison outcomes depicted that this in-tip SPME method was beneficial in terms of its simple, reusable, cost-saving setup together with a significant reduction in chemical consumption.
format Article
author Lu, Tan Lei
Shahriman, Mohamad Shariff
Sambasevam, Kavirajaa Pandian
Zain, Nur Nadhirah Mohamad
Yahaya, Noorfatimah
Lim, Vuanghao
Raoov, Muggundha
author_facet Lu, Tan Lei
Shahriman, Mohamad Shariff
Sambasevam, Kavirajaa Pandian
Zain, Nur Nadhirah Mohamad
Yahaya, Noorfatimah
Lim, Vuanghao
Raoov, Muggundha
author_sort Lu, Tan Lei
title In-tip solid-phase microextraction: a method for determination of sulphonamide residues in environmental water samples
title_short In-tip solid-phase microextraction: a method for determination of sulphonamide residues in environmental water samples
title_full In-tip solid-phase microextraction: a method for determination of sulphonamide residues in environmental water samples
title_fullStr In-tip solid-phase microextraction: a method for determination of sulphonamide residues in environmental water samples
title_full_unstemmed In-tip solid-phase microextraction: a method for determination of sulphonamide residues in environmental water samples
title_sort in-tip solid-phase microextraction: a method for determination of sulphonamide residues in environmental water samples
publisher Taylor & Francis
publishDate 2024
url http://eprints.um.edu.my/44340/
https://doi.org/10.1080/03067319.2021.2019721
_version_ 1805881162081501184
score 13.211869

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