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EDXRF and the relative presence of K, Ca, Fe and as in amyloidogenic tissues

Trace and minor elements play crucial roles in a variety of biological processes, including amyloid fibrils for-mation. Mechanisms include activation or inhibition of enzymatic reactions, competition between elements and metal proteins for binding positions, also changes to the permeability of cellu...

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Main Authors:	Ihsan, N. S. Mohd Nor, Sani, S. F. Abdul, Looi, Lai Meng, Pathmanathan, Dharini, Cheah, Phaik Leng, Chiew, S. F., Bradley, D. A.
Format:	Article
Published:	PERGAMON-ELSEVIER SCIENCE LTD 2024
Subjects:	RB Pathology
Online Access:	http://eprints.um.edu.my/44228/ https://doi.org/10.1016/j.saa.2023.123743
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spelling	my.um.eprints.442282024-06-25T06:48:33Z http://eprints.um.edu.my/44228/ EDXRF and the relative presence of K, Ca, Fe and as in amyloidogenic tissues Ihsan, N. S. Mohd Nor Sani, S. F. Abdul Looi, Lai Meng Pathmanathan, Dharini Cheah, Phaik Leng Chiew, S. F. Bradley, D. A. RB Pathology Trace and minor elements play crucial roles in a variety of biological processes, including amyloid fibrils for-mation. Mechanisms include activation or inhibition of enzymatic reactions, competition between elements and metal proteins for binding positions, also changes to the permeability of cellular membranes. These may influ-ence carcinogenic processes, with trace and minor element concentrations in normal and amyloid tissues potentially aiding in cancer diagnosis and etiology. With the analytical capability of the spectroscopic technique X-ray fluorescence (XRF), this can be used to detect and quantify the presence of elements in amyloid charac-terization, two of the trace elements known to be associated with amyloid fibrils. In present work, involving samples from a total of 22 subjects, samples of normal and amyloid-containing tissues of heart, kidney, thyroid, and other tissue organs were obtained, analyzed via energy-dispersive X-ray fluorescence (EDXRF). The elemental distribution of potassium (K), calcium (Ca), arsenic (As), and iron (Fe) was examined in both normal and amyloidogenic tissues using perpetual thin slices. In amyloidogenic tissues the levels of K, Ca, and Fe were found to be less than in corresponding normal tissues. Moreover, the presence of As was only observed in amyloidogenic samples; in a few cases in which there was an absence of As, amyloid samples were found to contain Fe. Analysis of arsenic in amyloid plaques has previously been difficult, often producing contradictory results. Using the present EDXRF facility we could distinguish between amyloidogenic and normal samples, with potential correlations in respect of the presence or concentration of specific elements. PERGAMON-ELSEVIER SCIENCE LTD 2024-03-05 Article PeerReviewed Ihsan, N. S. Mohd Nor and Sani, S. F. Abdul and Looi, Lai Meng and Pathmanathan, Dharini and Cheah, Phaik Leng and Chiew, S. F. and Bradley, D. A. (2024) EDXRF and the relative presence of K, Ca, Fe and as in amyloidogenic tissues. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 308. ISSN 1873-3557, DOI https://doi.org/10.1016/j.saa.2023.123743 <https://doi.org/10.1016/j.saa.2023.123743>. https://doi.org/10.1016/j.saa.2023.123743 10.1016/j.saa.2023.123743
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	RB Pathology
spellingShingle	RB Pathology Ihsan, N. S. Mohd Nor Sani, S. F. Abdul Looi, Lai Meng Pathmanathan, Dharini Cheah, Phaik Leng Chiew, S. F. Bradley, D. A. EDXRF and the relative presence of K, Ca, Fe and as in amyloidogenic tissues
description	Trace and minor elements play crucial roles in a variety of biological processes, including amyloid fibrils for-mation. Mechanisms include activation or inhibition of enzymatic reactions, competition between elements and metal proteins for binding positions, also changes to the permeability of cellular membranes. These may influ-ence carcinogenic processes, with trace and minor element concentrations in normal and amyloid tissues potentially aiding in cancer diagnosis and etiology. With the analytical capability of the spectroscopic technique X-ray fluorescence (XRF), this can be used to detect and quantify the presence of elements in amyloid charac-terization, two of the trace elements known to be associated with amyloid fibrils. In present work, involving samples from a total of 22 subjects, samples of normal and amyloid-containing tissues of heart, kidney, thyroid, and other tissue organs were obtained, analyzed via energy-dispersive X-ray fluorescence (EDXRF). The elemental distribution of potassium (K), calcium (Ca), arsenic (As), and iron (Fe) was examined in both normal and amyloidogenic tissues using perpetual thin slices. In amyloidogenic tissues the levels of K, Ca, and Fe were found to be less than in corresponding normal tissues. Moreover, the presence of As was only observed in amyloidogenic samples; in a few cases in which there was an absence of As, amyloid samples were found to contain Fe. Analysis of arsenic in amyloid plaques has previously been difficult, often producing contradictory results. Using the present EDXRF facility we could distinguish between amyloidogenic and normal samples, with potential correlations in respect of the presence or concentration of specific elements.
format	Article
author	Ihsan, N. S. Mohd Nor Sani, S. F. Abdul Looi, Lai Meng Pathmanathan, Dharini Cheah, Phaik Leng Chiew, S. F. Bradley, D. A.
author_facet	Ihsan, N. S. Mohd Nor Sani, S. F. Abdul Looi, Lai Meng Pathmanathan, Dharini Cheah, Phaik Leng Chiew, S. F. Bradley, D. A.
author_sort	Ihsan, N. S. Mohd Nor
title	EDXRF and the relative presence of K, Ca, Fe and as in amyloidogenic tissues
title_short	EDXRF and the relative presence of K, Ca, Fe and as in amyloidogenic tissues
title_full	EDXRF and the relative presence of K, Ca, Fe and as in amyloidogenic tissues
title_fullStr	EDXRF and the relative presence of K, Ca, Fe and as in amyloidogenic tissues
title_full_unstemmed	EDXRF and the relative presence of K, Ca, Fe and as in amyloidogenic tissues
title_sort	edxrf and the relative presence of k, ca, fe and as in amyloidogenic tissues
publisher	PERGAMON-ELSEVIER SCIENCE LTD
publishDate	2024
url	http://eprints.um.edu.my/44228/ https://doi.org/10.1016/j.saa.2023.123743
_version_	1805881138400460800
score	13.211869

EDXRF and the relative presence of K, Ca, Fe and as in amyloidogenic tissues

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