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Conjugated linoleic acid isomers induced dyslipidemia and lipoatrophy are exacerbated by rosiglitazone in ApoE null mice fed a Western diet

BACKGROUND: Insulin sensitizers have been used to treat Type 2 diabetes. However, their non-negligible side effects have led to cardiovascular concerns and the withdrawal of a member, rosiglitazone. OBJECTIVE: We combined conjugated linoleic acid (CLA) with rosiglitazone to test for amelioration of...

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Main Authors: Chai, Boon Kheng, Murugan, Dharmani Devi, Mustafa, Mohd Rais, Al-Shagga, Mustafa, Mohankumar, Suresh K.
Format: Article
Published: IOS Press BV 2022
Subjects:
RS Pharmacy and materia medica
Online Access:http://eprints.um.edu.my/43854/
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spelling my.um.eprints.438542023-12-29T07:06:04Z http://eprints.um.edu.my/43854/ Conjugated linoleic acid isomers induced dyslipidemia and lipoatrophy are exacerbated by rosiglitazone in ApoE null mice fed a Western diet Chai, Boon Kheng Murugan, Dharmani Devi Mustafa, Mohd Rais Al-Shagga, Mustafa Mohankumar, Suresh K. RS Pharmacy and materia medica BACKGROUND: Insulin sensitizers have been used to treat Type 2 diabetes. However, their non-negligible side effects have led to cardiovascular concerns and the withdrawal of a member, rosiglitazone. OBJECTIVE: We combined conjugated linoleic acid (CLA) with rosiglitazone to test for amelioration of side effects posed by rosiglitazone in vivo. METHODS: We utilized ApoE null mice fed with Western diet (WD) to test our hypothesis. Mice were fed WD, with or without CLA administration, for 12 weeks. CLA utilized in our study consisted of a 1:1 ratio of 95 pure c9,t11, and t10,c12 isomers at a concentration of 0.1 w/v in fat-free milk. Starting from Week 12, select mice received rosiglitazone. RESULTS: It was found that mice receiving CLA from Week 0 and rosiglitazone from Week 12 had the lowest body weight and exacerbated hepatomegaly. Although these mice had attenuated insulin resistance compared to mice receiving only Western diet, they display a marked increase in total plasma cholesterol and low-density lipoprotein (LDL) cholesterol. Mice receiving early CLA administration developed hyperleptinemia, which was not restored by rosiglitazone. CONCLUSION: Taken together, against the background of ApoE null genotype and WD feeding, simultaneous administration of 1:1 CLA and rosiglitazone led to dyslipidemic lipoatrophy. © 2022 - IOS Press. All rights reserved. IOS Press BV 2022 Article PeerReviewed Chai, Boon Kheng and Murugan, Dharmani Devi and Mustafa, Mohd Rais and Al-Shagga, Mustafa and Mohankumar, Suresh K. (2022) Conjugated linoleic acid isomers induced dyslipidemia and lipoatrophy are exacerbated by rosiglitazone in ApoE null mice fed a Western diet. Mediterranean Journal of Nutrition and Metabolism, 15 (3). 345 – 359. ISSN 1973798X, DOI https://doi.org/10.3233/MNM-211562 <https://doi.org/10.3233/MNM-211562>. 10.3233/MNM-211562
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic RS Pharmacy and materia medica
spellingShingle RS Pharmacy and materia medica
Chai, Boon Kheng
Murugan, Dharmani Devi
Mustafa, Mohd Rais
Al-Shagga, Mustafa
Mohankumar, Suresh K.
Conjugated linoleic acid isomers induced dyslipidemia and lipoatrophy are exacerbated by rosiglitazone in ApoE null mice fed a Western diet
description BACKGROUND: Insulin sensitizers have been used to treat Type 2 diabetes. However, their non-negligible side effects have led to cardiovascular concerns and the withdrawal of a member, rosiglitazone. OBJECTIVE: We combined conjugated linoleic acid (CLA) with rosiglitazone to test for amelioration of side effects posed by rosiglitazone in vivo. METHODS: We utilized ApoE null mice fed with Western diet (WD) to test our hypothesis. Mice were fed WD, with or without CLA administration, for 12 weeks. CLA utilized in our study consisted of a 1:1 ratio of 95 pure c9,t11, and t10,c12 isomers at a concentration of 0.1 w/v in fat-free milk. Starting from Week 12, select mice received rosiglitazone. RESULTS: It was found that mice receiving CLA from Week 0 and rosiglitazone from Week 12 had the lowest body weight and exacerbated hepatomegaly. Although these mice had attenuated insulin resistance compared to mice receiving only Western diet, they display a marked increase in total plasma cholesterol and low-density lipoprotein (LDL) cholesterol. Mice receiving early CLA administration developed hyperleptinemia, which was not restored by rosiglitazone. CONCLUSION: Taken together, against the background of ApoE null genotype and WD feeding, simultaneous administration of 1:1 CLA and rosiglitazone led to dyslipidemic lipoatrophy. © 2022 - IOS Press. All rights reserved.
format Article
author Chai, Boon Kheng
Murugan, Dharmani Devi
Mustafa, Mohd Rais
Al-Shagga, Mustafa
Mohankumar, Suresh K.
author_facet Chai, Boon Kheng
Murugan, Dharmani Devi
Mustafa, Mohd Rais
Al-Shagga, Mustafa
Mohankumar, Suresh K.
author_sort Chai, Boon Kheng
title Conjugated linoleic acid isomers induced dyslipidemia and lipoatrophy are exacerbated by rosiglitazone in ApoE null mice fed a Western diet
title_short Conjugated linoleic acid isomers induced dyslipidemia and lipoatrophy are exacerbated by rosiglitazone in ApoE null mice fed a Western diet
title_full Conjugated linoleic acid isomers induced dyslipidemia and lipoatrophy are exacerbated by rosiglitazone in ApoE null mice fed a Western diet
title_fullStr Conjugated linoleic acid isomers induced dyslipidemia and lipoatrophy are exacerbated by rosiglitazone in ApoE null mice fed a Western diet
title_full_unstemmed Conjugated linoleic acid isomers induced dyslipidemia and lipoatrophy are exacerbated by rosiglitazone in ApoE null mice fed a Western diet
title_sort conjugated linoleic acid isomers induced dyslipidemia and lipoatrophy are exacerbated by rosiglitazone in apoe null mice fed a western diet
publisher IOS Press BV
publishDate 2022
url http://eprints.um.edu.my/43854/
_version_ 1787133829874450432
score 13.211869

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