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In silico analysis of edible bird’s nest proteins as potential precursors for bioactive peptides

The present study aimed to perform an in silico evaluation of edible bird’s nest protein as potential precursors of bioactive peptides, as well as to determine whether such peptides can be released by selected proteolytic enzymes. Six edible bird’s nest (EBN) protein sequences from a previous study...

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Main Authors: Din, Khuzma, Amin, Amiza Mat, Ahmad, Fisal, Ismail, Amin, Shuib, Adawiyah Suriza
Format: Article
Published: 2022
Subjects:
Q Science (General)
Online Access:http://eprints.um.edu.my/43577/
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85134018571&doi=10.55230%2fmabjournal.v51i2.1997&partnerID=40&md5=26749f6011dcf1da7b1f97ac248de0fa
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spelling my.um.eprints.435772023-10-30T05:32:20Z http://eprints.um.edu.my/43577/ In silico analysis of edible bird’s nest proteins as potential precursors for bioactive peptides Din, Khuzma Amin, Amiza Mat Ahmad, Fisal Ismail, Amin Shuib, Adawiyah Suriza Q Science (General) The present study aimed to perform an in silico evaluation of edible bird’s nest protein as potential precursors of bioactive peptides, as well as to determine whether such peptides can be released by selected proteolytic enzymes. Six edible bird’s nest (EBN) protein sequences from a previous study were chosen as potential precursors to produce bioactive peptides via in silico method using the BIOPEP database. AMCase protein sequences gave the highest number of bioactivities (16 to 18) and nucleobindin-2 protein gave the lowest number of bioactivities (9) among the other protein sequences. It was found that the most potential bioactive peptides from EBN proteins are angiotensin-converting enzyme (ACE) inhibitors and dipeptidyl peptidase-IV (DPP-IV) inhibitors. Furthermore, in silico proteolysis using six selected enzymes was employed to release both dominant bioactivities in EBN proteins, which were ACE and DPP-IV inhibitors. This study shows that a combination of enzymes, chymotrypsin, and papain, produced the highest number of activities for both ACE and DPP-IV inhibitor peptides with the frequency of occurrence of bioactive peptides of 0.0968 and 0.1104, respectively. The toxic prediction tool, ToxinPred, found that all EBN peptides derived by in silico analysis were non-toxic. The current study proposed that EBN can serve as a potential source of bioactive peptides. © 2022, Malaysian Society of Applied Biology. All rights reserved. 2022 Article PeerReviewed Din, Khuzma and Amin, Amiza Mat and Ahmad, Fisal and Ismail, Amin and Shuib, Adawiyah Suriza (2022) In silico analysis of edible bird’s nest proteins as potential precursors for bioactive peptides. Malaysian Applied Biology, 51 (2). 53 -62. ISSN 0126-8643, DOI https://doi.org/10.55230/mabjournal.v51i2.1997 <https://doi.org/10.55230/mabjournal.v51i2.1997>. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85134018571&doi=10.55230%2fmabjournal.v51i2.1997&partnerID=40&md5=26749f6011dcf1da7b1f97ac248de0fa 10.55230/mabjournal.v51i2.1997
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic Q Science (General)
spellingShingle Q Science (General)
Din, Khuzma
Amin, Amiza Mat
Ahmad, Fisal
Ismail, Amin
Shuib, Adawiyah Suriza
In silico analysis of edible bird’s nest proteins as potential precursors for bioactive peptides
description The present study aimed to perform an in silico evaluation of edible bird’s nest protein as potential precursors of bioactive peptides, as well as to determine whether such peptides can be released by selected proteolytic enzymes. Six edible bird’s nest (EBN) protein sequences from a previous study were chosen as potential precursors to produce bioactive peptides via in silico method using the BIOPEP database. AMCase protein sequences gave the highest number of bioactivities (16 to 18) and nucleobindin-2 protein gave the lowest number of bioactivities (9) among the other protein sequences. It was found that the most potential bioactive peptides from EBN proteins are angiotensin-converting enzyme (ACE) inhibitors and dipeptidyl peptidase-IV (DPP-IV) inhibitors. Furthermore, in silico proteolysis using six selected enzymes was employed to release both dominant bioactivities in EBN proteins, which were ACE and DPP-IV inhibitors. This study shows that a combination of enzymes, chymotrypsin, and papain, produced the highest number of activities for both ACE and DPP-IV inhibitor peptides with the frequency of occurrence of bioactive peptides of 0.0968 and 0.1104, respectively. The toxic prediction tool, ToxinPred, found that all EBN peptides derived by in silico analysis were non-toxic. The current study proposed that EBN can serve as a potential source of bioactive peptides. © 2022, Malaysian Society of Applied Biology. All rights reserved.
format Article
author Din, Khuzma
Amin, Amiza Mat
Ahmad, Fisal
Ismail, Amin
Shuib, Adawiyah Suriza
author_facet Din, Khuzma
Amin, Amiza Mat
Ahmad, Fisal
Ismail, Amin
Shuib, Adawiyah Suriza
author_sort Din, Khuzma
title In silico analysis of edible bird’s nest proteins as potential precursors for bioactive peptides
title_short In silico analysis of edible bird’s nest proteins as potential precursors for bioactive peptides
title_full In silico analysis of edible bird’s nest proteins as potential precursors for bioactive peptides
title_fullStr In silico analysis of edible bird’s nest proteins as potential precursors for bioactive peptides
title_full_unstemmed In silico analysis of edible bird’s nest proteins as potential precursors for bioactive peptides
title_sort in silico analysis of edible bird’s nest proteins as potential precursors for bioactive peptides
publishDate 2022
url http://eprints.um.edu.my/43577/
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85134018571&doi=10.55230%2fmabjournal.v51i2.1997&partnerID=40&md5=26749f6011dcf1da7b1f97ac248de0fa
_version_ 1781704713697230848
score 13.211869

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