Staff View: Antibacterial activity of salvadora persica and benzylisothiocyanate against prevotella intermedia and eikenella corrodens incorporated into periodontal chips

Antibacterial activity of salvadora persica and benzylisothiocyanate against prevotella intermedia and eikenella corrodens incorporated into periodontal chips

Salvadora persica (S. persica) contains steam-distillable oil made of 90 Benzylisothiocyanate (BITC). It’s proven to have a wide range of bactericidal activity. The objectives of this study were to develop biodegradable periodontal chips containing S. persica, and BITC in a chitosan base and to eval...

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Main Authors:	AL-Bayaty, Fouad H., Razak, Muhammad Azly Abdul, Hussain, Saba F., Mulok, Tengku Z., Almas, Khalid, Smith, Steph, Abdulla, Mahmood A.
Format:	Article
Published:	University of Dicle 2022
Subjects:	R Medicine
Online Access:	http://eprints.um.edu.my/43571/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-85133795090&partnerID=40&md5=bdcb1ef20f8688c4f13fbe410a15a109
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spelling	my.um.eprints.435712023-10-30T03:01:32Z http://eprints.um.edu.my/43571/ Antibacterial activity of salvadora persica and benzylisothiocyanate against prevotella intermedia and eikenella corrodens incorporated into periodontal chips AL-Bayaty, Fouad H. Razak, Muhammad Azly Abdul Hussain, Saba F. Mulok, Tengku Z. Almas, Khalid Smith, Steph Abdulla, Mahmood A. R Medicine Salvadora persica (S. persica) contains steam-distillable oil made of 90 Benzylisothiocyanate (BITC). It’s proven to have a wide range of bactericidal activity. The objectives of this study were to develop biodegradable periodontal chips containing S. persica, and BITC in a chitosan base and to evaluate their antibacterial activity against Prevotella intermedia and Eikenella corrodens. Periodontal chips were developed that incorporated S. persica (Miswak) extract and BITC in a chitosan base. Sterile distilled water was used as negative control while chlohexidine was used as positive control. A gel diffusion test was performed to detect the potential antibacterial activity followed by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) Tests. Scanning electron microscopy (SEM) was used to identify the morphology and configuration of Prevotella intermedia and Eikenella corrodens bacteria before and after treatment with chips. The mean inhibition zone for the S. persica chips was found to be 9.0 mm. The MIC and MBC tests demostrated that all the concentrations for the chips succesfully inhibited the growth of the bacteria. Chlohexidine used as positive control also inhibited the bacteria. The negative control of sterile distilled water showed no inhibtion of bacterial growth. SEM showed that all chips produced distinct alterations to the bacterial morphology. E. corrodens and P. intermedia underwent shrinkage and formed irregular shapes. Periodontal chips formulated from S. persica and BITC incorporated into a chitosan base as a target drug delivery method, showed potent antibacterial activity against E. corrodens and P. intermedia. © 2022. Journal of International Dental and Medical Research. All Rights Reserved. University of Dicle 2022 Article PeerReviewed AL-Bayaty, Fouad H. and Razak, Muhammad Azly Abdul and Hussain, Saba F. and Mulok, Tengku Z. and Almas, Khalid and Smith, Steph and Abdulla, Mahmood A. (2022) Antibacterial activity of salvadora persica and benzylisothiocyanate against prevotella intermedia and eikenella corrodens incorporated into periodontal chips. Journal of International Dental and Medical Research, 15 (2). 498 -504. ISSN 1309-100X, https://www.scopus.com/inward/record.uri?eid=2-s2.0-85133795090&partnerID=40&md5=bdcb1ef20f8688c4f13fbe410a15a109
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	R Medicine
spellingShingle	R Medicine AL-Bayaty, Fouad H. Razak, Muhammad Azly Abdul Hussain, Saba F. Mulok, Tengku Z. Almas, Khalid Smith, Steph Abdulla, Mahmood A. Antibacterial activity of salvadora persica and benzylisothiocyanate against prevotella intermedia and eikenella corrodens incorporated into periodontal chips
description	Salvadora persica (S. persica) contains steam-distillable oil made of 90 Benzylisothiocyanate (BITC). It’s proven to have a wide range of bactericidal activity. The objectives of this study were to develop biodegradable periodontal chips containing S. persica, and BITC in a chitosan base and to evaluate their antibacterial activity against Prevotella intermedia and Eikenella corrodens. Periodontal chips were developed that incorporated S. persica (Miswak) extract and BITC in a chitosan base. Sterile distilled water was used as negative control while chlohexidine was used as positive control. A gel diffusion test was performed to detect the potential antibacterial activity followed by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) Tests. Scanning electron microscopy (SEM) was used to identify the morphology and configuration of Prevotella intermedia and Eikenella corrodens bacteria before and after treatment with chips. The mean inhibition zone for the S. persica chips was found to be 9.0 mm. The MIC and MBC tests demostrated that all the concentrations for the chips succesfully inhibited the growth of the bacteria. Chlohexidine used as positive control also inhibited the bacteria. The negative control of sterile distilled water showed no inhibtion of bacterial growth. SEM showed that all chips produced distinct alterations to the bacterial morphology. E. corrodens and P. intermedia underwent shrinkage and formed irregular shapes. Periodontal chips formulated from S. persica and BITC incorporated into a chitosan base as a target drug delivery method, showed potent antibacterial activity against E. corrodens and P. intermedia. © 2022. Journal of International Dental and Medical Research. All Rights Reserved.
format	Article
author	AL-Bayaty, Fouad H. Razak, Muhammad Azly Abdul Hussain, Saba F. Mulok, Tengku Z. Almas, Khalid Smith, Steph Abdulla, Mahmood A.
author_facet	AL-Bayaty, Fouad H. Razak, Muhammad Azly Abdul Hussain, Saba F. Mulok, Tengku Z. Almas, Khalid Smith, Steph Abdulla, Mahmood A.
author_sort	AL-Bayaty, Fouad H.
title	Antibacterial activity of salvadora persica and benzylisothiocyanate against prevotella intermedia and eikenella corrodens incorporated into periodontal chips
title_short	Antibacterial activity of salvadora persica and benzylisothiocyanate against prevotella intermedia and eikenella corrodens incorporated into periodontal chips
title_full	Antibacterial activity of salvadora persica and benzylisothiocyanate against prevotella intermedia and eikenella corrodens incorporated into periodontal chips
title_fullStr	Antibacterial activity of salvadora persica and benzylisothiocyanate against prevotella intermedia and eikenella corrodens incorporated into periodontal chips
title_full_unstemmed	Antibacterial activity of salvadora persica and benzylisothiocyanate against prevotella intermedia and eikenella corrodens incorporated into periodontal chips
title_sort	antibacterial activity of salvadora persica and benzylisothiocyanate against prevotella intermedia and eikenella corrodens incorporated into periodontal chips
publisher	University of Dicle
publishDate	2022
url	http://eprints.um.edu.my/43571/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-85133795090&partnerID=40&md5=bdcb1ef20f8688c4f13fbe410a15a109
_version_	1781704712843689984
score	13.211869

Antibacterial activity of salvadora persica and benzylisothiocyanate against prevotella intermedia and eikenella corrodens incorporated into periodontal chips

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