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RNA purification-free detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Background: Current diagnosis of SARS-CoV-2 infection relies on RNA purification prior to amplification. Typical extraction methods limit the processing speed and turnaround time for SARS-CoV-2 diagnostic testing. Methods: Here, we applied reverse transcription loop-mediated isothermal amplification...

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Main Authors: Lai, Meng Yee, Suppiah, Jeyanthi, Thayan, Ravindran, Ismail, Ilyiana, Mustapa, Nur Izati, Soh, Tuan Suhaila Tuan, Hassan, Afifah Haji, Peariasamy, Kalaiarasu M., Lee, Yee Leng, Lau, Yee Ling
Format: Article
Published: BioMed Central Ltd 2022
Subjects:
R Medicine (General)
RV Botanic, Thomsonian, and eclectic medicine
Online Access:http://eprints.um.edu.my/43188/
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spelling my.um.eprints.431882023-09-28T12:46:35Z http://eprints.um.edu.my/43188/ RNA purification-free detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP) Lai, Meng Yee Suppiah, Jeyanthi Thayan, Ravindran Ismail, Ilyiana Mustapa, Nur Izati Soh, Tuan Suhaila Tuan Hassan, Afifah Haji Peariasamy, Kalaiarasu M. Lee, Yee Leng Lau, Yee Ling R Medicine (General) RV Botanic, Thomsonian, and eclectic medicine Background: Current diagnosis of SARS-CoV-2 infection relies on RNA purification prior to amplification. Typical extraction methods limit the processing speed and turnaround time for SARS-CoV-2 diagnostic testing. Methods: Here, we applied reverse transcription loop-mediated isothermal amplification directly onto human clinical swabs samples to amplify the RNA from SARS-CoV-2 swab samples after processing with chelating resin. Results: By testing our method on 64 samples, we managed to develop an RT-LAMP assay with 95.9 sensitivity (95 CI 86 to 99.5) and 100 specificity (95 CI 78.2–100). Conclusion: The entire process including sample processing can be completed in approximately 50 min. This method has promising potential to be applied as a fast, simple and inexpensive diagnostic tool for the detection of SARS-CoV-2. © 2022, The Author(s). BioMed Central Ltd 2022-01-04 Article PeerReviewed Lai, Meng Yee and Suppiah, Jeyanthi and Thayan, Ravindran and Ismail, Ilyiana and Mustapa, Nur Izati and Soh, Tuan Suhaila Tuan and Hassan, Afifah Haji and Peariasamy, Kalaiarasu M. and Lee, Yee Leng and Lau, Yee Ling (2022) RNA purification-free detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP). Tropical Medicine and Health, 50 (1). ISSN 1348-8945, DOI https://doi.org/10.1186/s41182-021-00396-y <https://doi.org/10.1186/s41182-021-00396-y>. 10.1186/s41182-021-00396-y
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine (General)
RV Botanic, Thomsonian, and eclectic medicine
spellingShingle R Medicine (General)
RV Botanic, Thomsonian, and eclectic medicine
Lai, Meng Yee
Suppiah, Jeyanthi
Thayan, Ravindran
Ismail, Ilyiana
Mustapa, Nur Izati
Soh, Tuan Suhaila Tuan
Hassan, Afifah Haji
Peariasamy, Kalaiarasu M.
Lee, Yee Leng
Lau, Yee Ling
RNA purification-free detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)
description Background: Current diagnosis of SARS-CoV-2 infection relies on RNA purification prior to amplification. Typical extraction methods limit the processing speed and turnaround time for SARS-CoV-2 diagnostic testing. Methods: Here, we applied reverse transcription loop-mediated isothermal amplification directly onto human clinical swabs samples to amplify the RNA from SARS-CoV-2 swab samples after processing with chelating resin. Results: By testing our method on 64 samples, we managed to develop an RT-LAMP assay with 95.9 sensitivity (95 CI 86 to 99.5) and 100 specificity (95 CI 78.2–100). Conclusion: The entire process including sample processing can be completed in approximately 50 min. This method has promising potential to be applied as a fast, simple and inexpensive diagnostic tool for the detection of SARS-CoV-2. © 2022, The Author(s).
format Article
author Lai, Meng Yee
Suppiah, Jeyanthi
Thayan, Ravindran
Ismail, Ilyiana
Mustapa, Nur Izati
Soh, Tuan Suhaila Tuan
Hassan, Afifah Haji
Peariasamy, Kalaiarasu M.
Lee, Yee Leng
Lau, Yee Ling
author_facet Lai, Meng Yee
Suppiah, Jeyanthi
Thayan, Ravindran
Ismail, Ilyiana
Mustapa, Nur Izati
Soh, Tuan Suhaila Tuan
Hassan, Afifah Haji
Peariasamy, Kalaiarasu M.
Lee, Yee Leng
Lau, Yee Ling
author_sort Lai, Meng Yee
title RNA purification-free detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)
title_short RNA purification-free detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)
title_full RNA purification-free detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)
title_fullStr RNA purification-free detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)
title_full_unstemmed RNA purification-free detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)
title_sort rna purification-free detection of sars-cov-2 using reverse transcription loop-mediated isothermal amplification (rt-lamp)
publisher BioMed Central Ltd
publishDate 2022
url http://eprints.um.edu.my/43188/
_version_ 1781704673863925760
score 13.211869

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