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Molecular identification and biofilm-forming ability of Elizabethkingia species

Recently, Elizabethkingia species have gained attention as a cause of life-threatening infections. The identification via phenotypic methods of three important species-Elizabethkingia meningoseptica , E. anophelis and E. miricola is difficult. Our objectives were to re-assess 30 archived Flavobacter...

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Main Authors: Puah, Suat Moi, Fong, Sam Pei, Kee, Boon Pin, Puthucheary, S. D., Chua, Kek Heng
Format: Article
Published: Academic Press Ltd- Elsevier Science Ltd 2022
Subjects:
QR Microbiology
QR180 Immunology
Online Access:http://eprints.um.edu.my/43098/
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spelling my.um.eprints.430982023-08-28T07:48:48Z http://eprints.um.edu.my/43098/ Molecular identification and biofilm-forming ability of Elizabethkingia species Puah, Suat Moi Fong, Sam Pei Kee, Boon Pin Puthucheary, S. D. Chua, Kek Heng QR Microbiology QR180 Immunology Recently, Elizabethkingia species have gained attention as a cause of life-threatening infections. The identification via phenotypic methods of three important species-Elizabethkingia meningoseptica , E. anophelis and E. miricola is difficult. Our objectives were to re-assess 30 archived Flavobacterium meningosepticum isolates using 16S rRNA gene sequencing, ERIC-PCR, and biofilm formation assay. Twenty-four isolates were re-identified as E. anophelis and 6 as E. miricola. All of them had the ability to form biofilm as shown in microtiter plate assay based on crystal violet staining. Overall, E. anophelis had a higher specific biofilm formation index compared to E. miricola. A total of 42% (10 out of 24) of E. anophelis were classified as strong, 29% (7 out of 24) as moderate and 29% (7 out of 24) as weak biofilm producers. E. miricola, 17% (1 out of 6) isolates were strong biofilm producers, 50% (3 out of 6) moderate and 33% (2 out of 6) were weak producers. E. anophelis from tracheal secretions were significantly associated with (p = 0.0361) strong biofilm formation. In summary, this study showed that the isolates originally identified as F. meningosepticum were re-classified using the 16S rRNA gene as one of two Elizabethkingia species. The ability of E. anophelis to form strong biofilm in endotracheal tubes indicates their probable role in the pathogenesis of Elizabethkingia infections. Academic Press Ltd- Elsevier Science Ltd 2022-01 Article PeerReviewed Puah, Suat Moi and Fong, Sam Pei and Kee, Boon Pin and Puthucheary, S. D. and Chua, Kek Heng (2022) Molecular identification and biofilm-forming ability of Elizabethkingia species. Microbial Pathogenesis, 162. ISSN 0882-4010, DOI https://doi.org/10.1016/j.micpath.2021.105345 <https://doi.org/10.1016/j.micpath.2021.105345>. 10.1016/j.micpath.2021.105345
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QR Microbiology
QR180 Immunology
spellingShingle QR Microbiology
QR180 Immunology
Puah, Suat Moi
Fong, Sam Pei
Kee, Boon Pin
Puthucheary, S. D.
Chua, Kek Heng
Molecular identification and biofilm-forming ability of Elizabethkingia species
description Recently, Elizabethkingia species have gained attention as a cause of life-threatening infections. The identification via phenotypic methods of three important species-Elizabethkingia meningoseptica , E. anophelis and E. miricola is difficult. Our objectives were to re-assess 30 archived Flavobacterium meningosepticum isolates using 16S rRNA gene sequencing, ERIC-PCR, and biofilm formation assay. Twenty-four isolates were re-identified as E. anophelis and 6 as E. miricola. All of them had the ability to form biofilm as shown in microtiter plate assay based on crystal violet staining. Overall, E. anophelis had a higher specific biofilm formation index compared to E. miricola. A total of 42% (10 out of 24) of E. anophelis were classified as strong, 29% (7 out of 24) as moderate and 29% (7 out of 24) as weak biofilm producers. E. miricola, 17% (1 out of 6) isolates were strong biofilm producers, 50% (3 out of 6) moderate and 33% (2 out of 6) were weak producers. E. anophelis from tracheal secretions were significantly associated with (p = 0.0361) strong biofilm formation. In summary, this study showed that the isolates originally identified as F. meningosepticum were re-classified using the 16S rRNA gene as one of two Elizabethkingia species. The ability of E. anophelis to form strong biofilm in endotracheal tubes indicates their probable role in the pathogenesis of Elizabethkingia infections.
format Article
author Puah, Suat Moi
Fong, Sam Pei
Kee, Boon Pin
Puthucheary, S. D.
Chua, Kek Heng
author_facet Puah, Suat Moi
Fong, Sam Pei
Kee, Boon Pin
Puthucheary, S. D.
Chua, Kek Heng
author_sort Puah, Suat Moi
title Molecular identification and biofilm-forming ability of Elizabethkingia species
title_short Molecular identification and biofilm-forming ability of Elizabethkingia species
title_full Molecular identification and biofilm-forming ability of Elizabethkingia species
title_fullStr Molecular identification and biofilm-forming ability of Elizabethkingia species
title_full_unstemmed Molecular identification and biofilm-forming ability of Elizabethkingia species
title_sort molecular identification and biofilm-forming ability of elizabethkingia species
publisher Academic Press Ltd- Elsevier Science Ltd
publishDate 2022
url http://eprints.um.edu.my/43098/
_version_ 1776247442121949184
score 13.211869

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