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Comparative analysis of ITS1 nucleotide sequence reveals distinct genetic difference between Brugia Malayi from Northeast Borneo and Thailand

Brugia malayi is one of the parasitic worms which causes lymphatic filariasis in humans. Its geographical distribution includes a large part of Asia. Despite its wide distribution, very little is known about the genetic variation and molecular epidemiology of this species. In this study, the interna...

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Main Authors: Fong, M.Y., Noordin, R., Lau, Y.L., Cheong, F.W., Yunus, M.H., Idris, Z.M.
Format: Article
Language:English
Published: 2012
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/4245/1/Comparative_analysis_of_ITS1_nucleotide_sequence_reveals_distinct_genetic_difference_between_Brugia_malayi_from_Northeast_Borneo_and_Thailand.pdf
http://eprints.um.edu.my/4245/
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spelling my.um.eprints.42452013-01-08T01:46:18Z http://eprints.um.edu.my/4245/ Comparative analysis of ITS1 nucleotide sequence reveals distinct genetic difference between Brugia Malayi from Northeast Borneo and Thailand Fong, M.Y. Noordin, R. Lau, Y.L. Cheong, F.W. Yunus, M.H. Idris, Z.M. R Medicine Brugia malayi is one of the parasitic worms which causes lymphatic filariasis in humans. Its geographical distribution includes a large part of Asia. Despite its wide distribution, very little is known about the genetic variation and molecular epidemiology of this species. In this study, the internal transcribed spacer 1 (ITS1) nucleotide sequences of B. malayi from microfilaria-positive human blood samples in Northeast Borneo Island were determined, and compared with published ITS1 sequences of B. malayi isolated from cats and humans in Thailand. Multiple alignment analysis revealed that B. malayi ITS1 sequences from Northeast Borneo were more similar to each other than to those from Thailand. Phylogenetic trees inferred using Neighbour-Joining and Maximum Parsimony methods showed similar topology, with 2 distinct B. malayi clusters. The first cluster consisted of Northeast Borneo B. malayi isolates, whereas the second consisted of the Thailand isolates. The findings of this study suggest that B. malayi in Borneo Island has diverged significantly from those of mainland Asia, and this has implications for the diagnosis of B. malayi infection across the region using ITS1-based molecular techniques. 2012 Article PeerReviewed application/pdf en http://eprints.um.edu.my/4245/1/Comparative_analysis_of_ITS1_nucleotide_sequence_reveals_distinct_genetic_difference_between_Brugia_malayi_from_Northeast_Borneo_and_Thailand.pdf Fong, M.Y. and Noordin, R. and Lau, Y.L. and Cheong, F.W. and Yunus, M.H. and Idris, Z.M. (2012) Comparative analysis of ITS1 nucleotide sequence reveals distinct genetic difference between Brugia Malayi from Northeast Borneo and Thailand. Parasitology. p. 1. ISSN 1469-8161 22917270
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic R Medicine
spellingShingle R Medicine
Fong, M.Y.
Noordin, R.
Lau, Y.L.
Cheong, F.W.
Yunus, M.H.
Idris, Z.M.
Comparative analysis of ITS1 nucleotide sequence reveals distinct genetic difference between Brugia Malayi from Northeast Borneo and Thailand
description Brugia malayi is one of the parasitic worms which causes lymphatic filariasis in humans. Its geographical distribution includes a large part of Asia. Despite its wide distribution, very little is known about the genetic variation and molecular epidemiology of this species. In this study, the internal transcribed spacer 1 (ITS1) nucleotide sequences of B. malayi from microfilaria-positive human blood samples in Northeast Borneo Island were determined, and compared with published ITS1 sequences of B. malayi isolated from cats and humans in Thailand. Multiple alignment analysis revealed that B. malayi ITS1 sequences from Northeast Borneo were more similar to each other than to those from Thailand. Phylogenetic trees inferred using Neighbour-Joining and Maximum Parsimony methods showed similar topology, with 2 distinct B. malayi clusters. The first cluster consisted of Northeast Borneo B. malayi isolates, whereas the second consisted of the Thailand isolates. The findings of this study suggest that B. malayi in Borneo Island has diverged significantly from those of mainland Asia, and this has implications for the diagnosis of B. malayi infection across the region using ITS1-based molecular techniques.
format Article
author Fong, M.Y.
Noordin, R.
Lau, Y.L.
Cheong, F.W.
Yunus, M.H.
Idris, Z.M.
author_facet Fong, M.Y.
Noordin, R.
Lau, Y.L.
Cheong, F.W.
Yunus, M.H.
Idris, Z.M.
author_sort Fong, M.Y.
title Comparative analysis of ITS1 nucleotide sequence reveals distinct genetic difference between Brugia Malayi from Northeast Borneo and Thailand
title_short Comparative analysis of ITS1 nucleotide sequence reveals distinct genetic difference between Brugia Malayi from Northeast Borneo and Thailand
title_full Comparative analysis of ITS1 nucleotide sequence reveals distinct genetic difference between Brugia Malayi from Northeast Borneo and Thailand
title_fullStr Comparative analysis of ITS1 nucleotide sequence reveals distinct genetic difference between Brugia Malayi from Northeast Borneo and Thailand
title_full_unstemmed Comparative analysis of ITS1 nucleotide sequence reveals distinct genetic difference between Brugia Malayi from Northeast Borneo and Thailand
title_sort comparative analysis of its1 nucleotide sequence reveals distinct genetic difference between brugia malayi from northeast borneo and thailand
publishDate 2012
url http://eprints.um.edu.my/4245/1/Comparative_analysis_of_ITS1_nucleotide_sequence_reveals_distinct_genetic_difference_between_Brugia_malayi_from_Northeast_Borneo_and_Thailand.pdf
http://eprints.um.edu.my/4245/
_version_ 1643687292599009280
score 13.211869

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