Staff View: Polyphenol-capped biogenic synthesis of noble metallic silver nanoparticles for antifungal activity against candida auris

Polyphenol-capped biogenic synthesis of noble metallic silver nanoparticles for antifungal activity against candida auris

In terms of reduced toxicity, the biologically inspired green synthesis of nanoparticles has emerged as a promising alternative to chemically fabricated nanoparticles. The use of a highly stable, biocompatible, and environmentally friendly aqueous extract of Cynara cardunculus as a reducing and capp...

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Main Authors:	Malik, Maqsood Ahmad, Batterjee, Maha G., Kamli, Majid Rasool, Alzahrani, Khalid Ahmed, Danish, Ekram Y., Nabi, Arshid
Format:	Article
Published:	MDPI 2022
Subjects:	QR Microbiology
Online Access:	http://eprints.um.edu.my/41958/
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spelling	my.um.eprints.419582023-10-17T01:22:32Z http://eprints.um.edu.my/41958/ Polyphenol-capped biogenic synthesis of noble metallic silver nanoparticles for antifungal activity against candida auris Malik, Maqsood Ahmad Batterjee, Maha G. Kamli, Majid Rasool Alzahrani, Khalid Ahmed Danish, Ekram Y. Nabi, Arshid QR Microbiology In terms of reduced toxicity, the biologically inspired green synthesis of nanoparticles has emerged as a promising alternative to chemically fabricated nanoparticles. The use of a highly stable, biocompatible, and environmentally friendly aqueous extract of Cynara cardunculus as a reducing and capping agent in this study demonstrated the possibility of green manufacturing of silver nanoparticles (CC-AgNPs). UV-visible spectroscopy validated the development of CC-AgNPs, indicating the surface plasmon resonance (SPR) lambda(max) band at 438 nm. The band gap of CC-AgNPs was found to be 2.26 eV. SEM and TEM analysis examined the surface morphology of CC-AgNPs, and micrographs revealed that the nanoparticles were spherical. The crystallinity, crystallite size, and phase purity of as-prepared nanoparticles were confirmed using XRD analysis, and it was confirmed that the CC-AgNPs were a face-centered cubic (fcc) crystalline-structured material. Furthermore, the role of active functional groups involved in the reduction and surface capping of CC-AgNPs was revealed using the Fourier transform infrared (FTIR) spectroscopic technique. CC-AgNPs were mostly spherical and monodispersed, with an average size of 26.89 nm, and were shown to be stable for a longer period without any noticeable change at room temperature. Further, we checked the antifungal mechanism of CC-AgNPs against C. auris MRL6057. The minimum inhibitory concentrations (MIC) and minimum fungicidal concentrations (MFC) were 50.0 mu g/mL and 100.0 mu g/mL respectively. The cell count and viability assay confirmed the fungicidal potential of CC-AgNPs. Further, the analysis showed that CC-AgNPs could induce apoptosis and G2/M phase cell cycle arrest in C. auris MRL6057. Our results also suggest that the CC-AgNPs were responsible for the induction of mitochondrial toxicity. TUNEL assay results revealed that higher concentrations of CC-AgNPs could cause DNA fragmentation. Therefore, the present study suggested that CC-AgNPs hold the capacity for antifungal drug development against C. auris infections. MDPI 2022-06 Article PeerReviewed Malik, Maqsood Ahmad and Batterjee, Maha G. and Kamli, Majid Rasool and Alzahrani, Khalid Ahmed and Danish, Ekram Y. and Nabi, Arshid (2022) Polyphenol-capped biogenic synthesis of noble metallic silver nanoparticles for antifungal activity against candida auris. Journal of Fungi, 8 (6). ISSN 2309-608X, DOI https://doi.org/10.3390/jof8060639 <https://doi.org/10.3390/jof8060639>. 10.3390/jof8060639
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	QR Microbiology
spellingShingle	QR Microbiology Malik, Maqsood Ahmad Batterjee, Maha G. Kamli, Majid Rasool Alzahrani, Khalid Ahmed Danish, Ekram Y. Nabi, Arshid Polyphenol-capped biogenic synthesis of noble metallic silver nanoparticles for antifungal activity against candida auris
description	In terms of reduced toxicity, the biologically inspired green synthesis of nanoparticles has emerged as a promising alternative to chemically fabricated nanoparticles. The use of a highly stable, biocompatible, and environmentally friendly aqueous extract of Cynara cardunculus as a reducing and capping agent in this study demonstrated the possibility of green manufacturing of silver nanoparticles (CC-AgNPs). UV-visible spectroscopy validated the development of CC-AgNPs, indicating the surface plasmon resonance (SPR) lambda(max) band at 438 nm. The band gap of CC-AgNPs was found to be 2.26 eV. SEM and TEM analysis examined the surface morphology of CC-AgNPs, and micrographs revealed that the nanoparticles were spherical. The crystallinity, crystallite size, and phase purity of as-prepared nanoparticles were confirmed using XRD analysis, and it was confirmed that the CC-AgNPs were a face-centered cubic (fcc) crystalline-structured material. Furthermore, the role of active functional groups involved in the reduction and surface capping of CC-AgNPs was revealed using the Fourier transform infrared (FTIR) spectroscopic technique. CC-AgNPs were mostly spherical and monodispersed, with an average size of 26.89 nm, and were shown to be stable for a longer period without any noticeable change at room temperature. Further, we checked the antifungal mechanism of CC-AgNPs against C. auris MRL6057. The minimum inhibitory concentrations (MIC) and minimum fungicidal concentrations (MFC) were 50.0 mu g/mL and 100.0 mu g/mL respectively. The cell count and viability assay confirmed the fungicidal potential of CC-AgNPs. Further, the analysis showed that CC-AgNPs could induce apoptosis and G2/M phase cell cycle arrest in C. auris MRL6057. Our results also suggest that the CC-AgNPs were responsible for the induction of mitochondrial toxicity. TUNEL assay results revealed that higher concentrations of CC-AgNPs could cause DNA fragmentation. Therefore, the present study suggested that CC-AgNPs hold the capacity for antifungal drug development against C. auris infections.
format	Article
author	Malik, Maqsood Ahmad Batterjee, Maha G. Kamli, Majid Rasool Alzahrani, Khalid Ahmed Danish, Ekram Y. Nabi, Arshid
author_facet	Malik, Maqsood Ahmad Batterjee, Maha G. Kamli, Majid Rasool Alzahrani, Khalid Ahmed Danish, Ekram Y. Nabi, Arshid
author_sort	Malik, Maqsood Ahmad
title	Polyphenol-capped biogenic synthesis of noble metallic silver nanoparticles for antifungal activity against candida auris
title_short	Polyphenol-capped biogenic synthesis of noble metallic silver nanoparticles for antifungal activity against candida auris
title_full	Polyphenol-capped biogenic synthesis of noble metallic silver nanoparticles for antifungal activity against candida auris
title_fullStr	Polyphenol-capped biogenic synthesis of noble metallic silver nanoparticles for antifungal activity against candida auris
title_full_unstemmed	Polyphenol-capped biogenic synthesis of noble metallic silver nanoparticles for antifungal activity against candida auris
title_sort	polyphenol-capped biogenic synthesis of noble metallic silver nanoparticles for antifungal activity against candida auris
publisher	MDPI
publishDate	2022
url	http://eprints.um.edu.my/41958/
_version_	1781704576127205376
score	13.211869

Polyphenol-capped biogenic synthesis of noble metallic silver nanoparticles for antifungal activity against candida auris

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