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Gallocatechin-silver nanoparticles embedded in cotton gauze patches accelerated wound healing in diabetic rats by promoting proliferation and inhibiting apoptosis through the Wnt/beta-catenin signaling pathway

Background: Diabetes mellitus is a chronic metabolic disorder characterized by elevated plasma glucose levels. It is often defined as a lifestyle disease having severe economic and physiological repercussions on the individual. One of the most prevalent clinical consequences of diabetes is the laggi...

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Main Authors: Reddy, Vendidandala Nagarjuna, Nyamathulla, Shaik, Pahirulzaman, Khomaizon Abdul Kadi, Mokhtar, Seri Intan, Nelli, Giribabu, Pasupulet, Visweswara Rao
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Published: Public Library of Science 2022
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R Medicine (General)
Online Access:http://eprints.um.edu.my/41796/
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spelling my.um.eprints.417962023-10-23T07:48:40Z http://eprints.um.edu.my/41796/ Gallocatechin-silver nanoparticles embedded in cotton gauze patches accelerated wound healing in diabetic rats by promoting proliferation and inhibiting apoptosis through the Wnt/beta-catenin signaling pathway Reddy, Vendidandala Nagarjuna Nyamathulla, Shaik Pahirulzaman, Khomaizon Abdul Kadi Mokhtar, Seri Intan Nelli, Giribabu Pasupulet, Visweswara Rao R Medicine (General) Background: Diabetes mellitus is a chronic metabolic disorder characterized by elevated plasma glucose levels. It is often defined as a lifestyle disease having severe economic and physiological repercussions on the individual. One of the most prevalent clinical consequences of diabetes is the lagging pace of injury healing leading to chronic wounds, which still to date have limited treatment options. The objective of this research is to look into the wound healing capabilities of gallocatechin (GC) and silver nanoparticles (AgNPs) impregnated patches in diabetic rats. Experimental rats were dressed patches and the wound healing skin region was dissected at the end of the experiment for molecular analysis. The wound healing rate in diabetic rats dressed with CGP2 and CGP3 & silver sulfadiazine (AgS) patches were found to be high. While mRNA and immunofluorescence or immunohistochemistry assays reveal that Wnt3a and beta-catenin levels were higher with Gsk-3 beta and c-fos levels were lower in diabetic rats dressed with in CGP2 and CGP3 as compared with diabetic rats dressed with DC+CGP1. Furthermore, apoptosis markers such as caspase-3, caspase-9, and Bax levels were reduced, whereas anti-apoptosis maker (Bcl-2) and proliferation marker (PCNA) levels were increased in diabetic rats dressed with CGP2 and CGP3 as compared with diabetic rats dressed with DC+CGP1. In conclusion, the results demonstrated that GC-AgNPs-CGP (CGP2 & CGP3) dressing on diabetes wound rats decreased changes in Wnt3a/beta-catenin pathways, resulting in lower apoptosis and greater proliferation, so drastically improving diabetic wound healing. Public Library of Science 2022 Article PeerReviewed Reddy, Vendidandala Nagarjuna and Nyamathulla, Shaik and Pahirulzaman, Khomaizon Abdul Kadi and Mokhtar, Seri Intan and Nelli, Giribabu and Pasupulet, Visweswara Rao (2022) Gallocatechin-silver nanoparticles embedded in cotton gauze patches accelerated wound healing in diabetic rats by promoting proliferation and inhibiting apoptosis through the Wnt/beta-catenin signaling pathway. PLoS ONE, 17 (6). ISSN 1932-6203, DOI https://doi.org/10.1371/journal.pone.0268505 <https://doi.org/10.1371/journal.pone.0268505>. 10.1371/journal.pone.0268505
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine (General)
spellingShingle R Medicine (General)
Reddy, Vendidandala Nagarjuna
Nyamathulla, Shaik
Pahirulzaman, Khomaizon Abdul Kadi
Mokhtar, Seri Intan
Nelli, Giribabu
Pasupulet, Visweswara Rao
Gallocatechin-silver nanoparticles embedded in cotton gauze patches accelerated wound healing in diabetic rats by promoting proliferation and inhibiting apoptosis through the Wnt/beta-catenin signaling pathway
description Background: Diabetes mellitus is a chronic metabolic disorder characterized by elevated plasma glucose levels. It is often defined as a lifestyle disease having severe economic and physiological repercussions on the individual. One of the most prevalent clinical consequences of diabetes is the lagging pace of injury healing leading to chronic wounds, which still to date have limited treatment options. The objective of this research is to look into the wound healing capabilities of gallocatechin (GC) and silver nanoparticles (AgNPs) impregnated patches in diabetic rats. Experimental rats were dressed patches and the wound healing skin region was dissected at the end of the experiment for molecular analysis. The wound healing rate in diabetic rats dressed with CGP2 and CGP3 & silver sulfadiazine (AgS) patches were found to be high. While mRNA and immunofluorescence or immunohistochemistry assays reveal that Wnt3a and beta-catenin levels were higher with Gsk-3 beta and c-fos levels were lower in diabetic rats dressed with in CGP2 and CGP3 as compared with diabetic rats dressed with DC+CGP1. Furthermore, apoptosis markers such as caspase-3, caspase-9, and Bax levels were reduced, whereas anti-apoptosis maker (Bcl-2) and proliferation marker (PCNA) levels were increased in diabetic rats dressed with CGP2 and CGP3 as compared with diabetic rats dressed with DC+CGP1. In conclusion, the results demonstrated that GC-AgNPs-CGP (CGP2 & CGP3) dressing on diabetes wound rats decreased changes in Wnt3a/beta-catenin pathways, resulting in lower apoptosis and greater proliferation, so drastically improving diabetic wound healing.
format Article
author Reddy, Vendidandala Nagarjuna
Nyamathulla, Shaik
Pahirulzaman, Khomaizon Abdul Kadi
Mokhtar, Seri Intan
Nelli, Giribabu
Pasupulet, Visweswara Rao
author_facet Reddy, Vendidandala Nagarjuna
Nyamathulla, Shaik
Pahirulzaman, Khomaizon Abdul Kadi
Mokhtar, Seri Intan
Nelli, Giribabu
Pasupulet, Visweswara Rao
author_sort Reddy, Vendidandala Nagarjuna
title Gallocatechin-silver nanoparticles embedded in cotton gauze patches accelerated wound healing in diabetic rats by promoting proliferation and inhibiting apoptosis through the Wnt/beta-catenin signaling pathway
title_short Gallocatechin-silver nanoparticles embedded in cotton gauze patches accelerated wound healing in diabetic rats by promoting proliferation and inhibiting apoptosis through the Wnt/beta-catenin signaling pathway
title_full Gallocatechin-silver nanoparticles embedded in cotton gauze patches accelerated wound healing in diabetic rats by promoting proliferation and inhibiting apoptosis through the Wnt/beta-catenin signaling pathway
title_fullStr Gallocatechin-silver nanoparticles embedded in cotton gauze patches accelerated wound healing in diabetic rats by promoting proliferation and inhibiting apoptosis through the Wnt/beta-catenin signaling pathway
title_full_unstemmed Gallocatechin-silver nanoparticles embedded in cotton gauze patches accelerated wound healing in diabetic rats by promoting proliferation and inhibiting apoptosis through the Wnt/beta-catenin signaling pathway
title_sort gallocatechin-silver nanoparticles embedded in cotton gauze patches accelerated wound healing in diabetic rats by promoting proliferation and inhibiting apoptosis through the wnt/beta-catenin signaling pathway
publisher Public Library of Science
publishDate 2022
url http://eprints.um.edu.my/41796/
_version_ 1781704557037879296
score 13.211869

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