Staff View: Fibrinogen isoforms as potential blood-based biomarkers of Alzheimer's disease using a proteomics approach

Fibrinogen isoforms as potential blood-based biomarkers of Alzheimer's disease using a proteomics approach

Objective: Alzheimer's disease (AD), the commonest form of dementia which is characterized by progressive decline in cognitive function, can only be definitively diagnosed after death. Although biomarkers may aid diagnosis, currently available AD biomarkers, which are predominantly based on cer...

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Main Authors:	Rehiman, Siti Hajar, Lim, Siong Meng, Lim, Fei Tieng, Chin, Ai-Vyrn, Tan, Maw Pin, Kamaruzzaman, Shahrul Bahyah, Ramasamy, Kalavathy, Abdul Majeed, Abu Bakar
Format:	Article
Published:	Taylor & Francis Ltd 2022
Subjects:	RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Online Access:	http://eprints.um.edu.my/41517/
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spelling	my.um.eprints.415172023-11-06T02:58:02Z http://eprints.um.edu.my/41517/ Fibrinogen isoforms as potential blood-based biomarkers of Alzheimer's disease using a proteomics approach Rehiman, Siti Hajar Lim, Siong Meng Lim, Fei Tieng Chin, Ai-Vyrn Tan, Maw Pin Kamaruzzaman, Shahrul Bahyah Ramasamy, Kalavathy Abdul Majeed, Abu Bakar RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry Objective: Alzheimer's disease (AD), the commonest form of dementia which is characterized by progressive decline in cognitive function, can only be definitively diagnosed after death. Although biomarkers may aid diagnosis, currently available AD biomarkers, which are predominantly based on cerebrospinal fluid and neuroimaging facilities, are either invasive or costly. Blood-based biomarkers for AD diagnosis are highly sought after due to its practicality at the clinic. This study was undertaken to determine the differential protein expression in plasma amongst Malaysian AD, mild cognitive impairment (MCI) and non-AD individuals. Methods: A proteomic approach which utilized two-dimensional differential in gel electrophoresis (2 D DIGE) was performed for blood samples from 15 AD, 14 MCI and 15 non-AD individuals. Results: Mass spectrometry (MS)-based protein identification via MALDI ToF/ToF showed that fibrinogen-beta-chain (spot 64) and fibrinogen-gamma-chain (spot 91) with differential expression ratio >1.5 were significantly upregulated (p < 0.05) in AD patients when compared to non-AD individuals. Further data analysis using Pearson correlation found that the upregulated fibrinogen-gamma-chain was weakly but significantly (p < 0.05) and inversely correlated with cognitive decline. Conclusion: Fibrinogen isoforms may play important roles in the vascular pathology of AD as well as neuroinflammation. As such, fibrinogen appears to be a promising blood-based biomarker for AD. Further validation of the present findings in larger population is now warranted. Taylor & Francis Ltd 2022-09-15 Article PeerReviewed Rehiman, Siti Hajar and Lim, Siong Meng and Lim, Fei Tieng and Chin, Ai-Vyrn and Tan, Maw Pin and Kamaruzzaman, Shahrul Bahyah and Ramasamy, Kalavathy and Abdul Majeed, Abu Bakar (2022) Fibrinogen isoforms as potential blood-based biomarkers of Alzheimer's disease using a proteomics approach. International Journal of Neuroscience, 132 (10). pp. 1014-1025. ISSN 0020-7454, DOI https://doi.org/10.1080/00207454.2020.1860038 <https://doi.org/10.1080/00207454.2020.1860038>. 10.1080/00207454.2020.1860038
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
spellingShingle	RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry Rehiman, Siti Hajar Lim, Siong Meng Lim, Fei Tieng Chin, Ai-Vyrn Tan, Maw Pin Kamaruzzaman, Shahrul Bahyah Ramasamy, Kalavathy Abdul Majeed, Abu Bakar Fibrinogen isoforms as potential blood-based biomarkers of Alzheimer's disease using a proteomics approach
description	Objective: Alzheimer's disease (AD), the commonest form of dementia which is characterized by progressive decline in cognitive function, can only be definitively diagnosed after death. Although biomarkers may aid diagnosis, currently available AD biomarkers, which are predominantly based on cerebrospinal fluid and neuroimaging facilities, are either invasive or costly. Blood-based biomarkers for AD diagnosis are highly sought after due to its practicality at the clinic. This study was undertaken to determine the differential protein expression in plasma amongst Malaysian AD, mild cognitive impairment (MCI) and non-AD individuals. Methods: A proteomic approach which utilized two-dimensional differential in gel electrophoresis (2 D DIGE) was performed for blood samples from 15 AD, 14 MCI and 15 non-AD individuals. Results: Mass spectrometry (MS)-based protein identification via MALDI ToF/ToF showed that fibrinogen-beta-chain (spot 64) and fibrinogen-gamma-chain (spot 91) with differential expression ratio >1.5 were significantly upregulated (p < 0.05) in AD patients when compared to non-AD individuals. Further data analysis using Pearson correlation found that the upregulated fibrinogen-gamma-chain was weakly but significantly (p < 0.05) and inversely correlated with cognitive decline. Conclusion: Fibrinogen isoforms may play important roles in the vascular pathology of AD as well as neuroinflammation. As such, fibrinogen appears to be a promising blood-based biomarker for AD. Further validation of the present findings in larger population is now warranted.
format	Article
author	Rehiman, Siti Hajar Lim, Siong Meng Lim, Fei Tieng Chin, Ai-Vyrn Tan, Maw Pin Kamaruzzaman, Shahrul Bahyah Ramasamy, Kalavathy Abdul Majeed, Abu Bakar
author_facet	Rehiman, Siti Hajar Lim, Siong Meng Lim, Fei Tieng Chin, Ai-Vyrn Tan, Maw Pin Kamaruzzaman, Shahrul Bahyah Ramasamy, Kalavathy Abdul Majeed, Abu Bakar
author_sort	Rehiman, Siti Hajar
title	Fibrinogen isoforms as potential blood-based biomarkers of Alzheimer's disease using a proteomics approach
title_short	Fibrinogen isoforms as potential blood-based biomarkers of Alzheimer's disease using a proteomics approach
title_full	Fibrinogen isoforms as potential blood-based biomarkers of Alzheimer's disease using a proteomics approach
title_fullStr	Fibrinogen isoforms as potential blood-based biomarkers of Alzheimer's disease using a proteomics approach
title_full_unstemmed	Fibrinogen isoforms as potential blood-based biomarkers of Alzheimer's disease using a proteomics approach
title_sort	fibrinogen isoforms as potential blood-based biomarkers of alzheimer's disease using a proteomics approach
publisher	Taylor & Francis Ltd
publishDate	2022
url	http://eprints.um.edu.my/41517/
_version_	1783876720854564864
score	13.211869

Fibrinogen isoforms as potential blood-based biomarkers of Alzheimer's disease using a proteomics approach

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