Staff View: Suppression of melanoma growth in a murine tumour model using orthosiphon stamineus benth. extract loaded in ethanolic phospholipid vesicles (spherosome)

Suppression of melanoma growth in a murine tumour model using orthosiphon stamineus benth. extract loaded in ethanolic phospholipid vesicles (spherosome)

Orthosiphon stamineus Benth (O.S) is a traditional south-east Asian herb. The extract of O.S is used in the formulation of ethanolic nanolipid vesicle system to have considerable potential for tumour therapeutics. Methods: The research objective is to develop and characterise the anticancer and anti...

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Main Authors:	Nazari, V. Mansoureh, Mahmood, Syed, Shah, Amin Malik, Al-Suede, Fouad Saleh Resq
Format:	Article
Published:	Bentham Science Publ Ltd 2022
Subjects:	QD Chemistry R Medicine RM Therapeutics. Pharmacology
Online Access:	http://eprints.um.edu.my/41471/
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spelling	my.um.eprints.414712023-09-25T03:09:10Z http://eprints.um.edu.my/41471/ Suppression of melanoma growth in a murine tumour model using orthosiphon stamineus benth. extract loaded in ethanolic phospholipid vesicles (spherosome) Nazari, V. Mansoureh Mahmood, Syed Shah, Amin Malik Al-Suede, Fouad Saleh Resq QD Chemistry R Medicine RM Therapeutics. Pharmacology Orthosiphon stamineus Benth (O.S) is a traditional south-east Asian herb. The extract of O.S is used in the formulation of ethanolic nanolipid vesicle system to have considerable potential for tumour therapeutics. Methods: The research objective is to develop and characterise the anticancer and antiangiogenic effect of O.S extract in the form of nano-ethanolic spherosomes (ESP) using phospholipids in melanoma. Spherosomes formulation of O.S was developed using the thin-film re-hydration method and converted to gel using Acrypol 1%. The formulations were subjected to optimisation and physical-chemical characterisations like particle size, surface charge, DSC, FTIR, and TEM. Cytotoxicity of O.S and ESP was studied using an endothelial cell line (EA. hy926). Furthermore, anti-melanoma effect of O.S spherosome gel was studied in albino mice after topical administration. Results: ESP-6 with the ratio of extract (O.S): cholesterol: phospholipid (1: 6: 0.5) showed the highest entrapment efficiency (80.56 +/- 0.84%) using ultraviolet spectroscopy. In-vivo permeation/penetration studies revealed deeper absorption of ESP-6 compared to a hydroethanolic gel of O.S. In-vitro and in vivo anti-melanoma studies demonstrated the significant tumour-suppressing effect of ESP-6 on murine melanoma. Percentage inhibition of tumour growth by O.S and ESP-6 at 3000 mg/kg showed to be 63.98 +/- 7.86% and 87.76 +/- 7.90%, respectively. Conclusion: Spherosome vesicles were developed with a smooth surface. The results demonstrated that O.S extract showed no toxicity when tested on the endothelial cell line. O.S loaded in spherosomes has the potential to lower the growth of melanoma in mice. The spherosomes loaded with O.S do not promote tumour growth or act as antiangio-genetic in melanoma. Bentham Science Publ Ltd 2022-04 Article PeerReviewed Nazari, V. Mansoureh and Mahmood, Syed and Shah, Amin Malik and Al-Suede, Fouad Saleh Resq (2022) Suppression of melanoma growth in a murine tumour model using orthosiphon stamineus benth. extract loaded in ethanolic phospholipid vesicles (spherosome). Current Drug Metabolism, 23 (4). pp. 317-328. ISSN 1389-2002, DOI https://doi.org/10.2174/1389200223666220416215129 <https://doi.org/10.2174/1389200223666220416215129>. 10.2174/1389200223666220416215129
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	QD Chemistry R Medicine RM Therapeutics. Pharmacology
spellingShingle	QD Chemistry R Medicine RM Therapeutics. Pharmacology Nazari, V. Mansoureh Mahmood, Syed Shah, Amin Malik Al-Suede, Fouad Saleh Resq Suppression of melanoma growth in a murine tumour model using orthosiphon stamineus benth. extract loaded in ethanolic phospholipid vesicles (spherosome)
description	Orthosiphon stamineus Benth (O.S) is a traditional south-east Asian herb. The extract of O.S is used in the formulation of ethanolic nanolipid vesicle system to have considerable potential for tumour therapeutics. Methods: The research objective is to develop and characterise the anticancer and antiangiogenic effect of O.S extract in the form of nano-ethanolic spherosomes (ESP) using phospholipids in melanoma. Spherosomes formulation of O.S was developed using the thin-film re-hydration method and converted to gel using Acrypol 1%. The formulations were subjected to optimisation and physical-chemical characterisations like particle size, surface charge, DSC, FTIR, and TEM. Cytotoxicity of O.S and ESP was studied using an endothelial cell line (EA. hy926). Furthermore, anti-melanoma effect of O.S spherosome gel was studied in albino mice after topical administration. Results: ESP-6 with the ratio of extract (O.S): cholesterol: phospholipid (1: 6: 0.5) showed the highest entrapment efficiency (80.56 +/- 0.84%) using ultraviolet spectroscopy. In-vivo permeation/penetration studies revealed deeper absorption of ESP-6 compared to a hydroethanolic gel of O.S. In-vitro and in vivo anti-melanoma studies demonstrated the significant tumour-suppressing effect of ESP-6 on murine melanoma. Percentage inhibition of tumour growth by O.S and ESP-6 at 3000 mg/kg showed to be 63.98 +/- 7.86% and 87.76 +/- 7.90%, respectively. Conclusion: Spherosome vesicles were developed with a smooth surface. The results demonstrated that O.S extract showed no toxicity when tested on the endothelial cell line. O.S loaded in spherosomes has the potential to lower the growth of melanoma in mice. The spherosomes loaded with O.S do not promote tumour growth or act as antiangio-genetic in melanoma.
format	Article
author	Nazari, V. Mansoureh Mahmood, Syed Shah, Amin Malik Al-Suede, Fouad Saleh Resq
author_facet	Nazari, V. Mansoureh Mahmood, Syed Shah, Amin Malik Al-Suede, Fouad Saleh Resq
author_sort	Nazari, V. Mansoureh
title	Suppression of melanoma growth in a murine tumour model using orthosiphon stamineus benth. extract loaded in ethanolic phospholipid vesicles (spherosome)
title_short	Suppression of melanoma growth in a murine tumour model using orthosiphon stamineus benth. extract loaded in ethanolic phospholipid vesicles (spherosome)
title_full	Suppression of melanoma growth in a murine tumour model using orthosiphon stamineus benth. extract loaded in ethanolic phospholipid vesicles (spherosome)
title_fullStr	Suppression of melanoma growth in a murine tumour model using orthosiphon stamineus benth. extract loaded in ethanolic phospholipid vesicles (spherosome)
title_full_unstemmed	Suppression of melanoma growth in a murine tumour model using orthosiphon stamineus benth. extract loaded in ethanolic phospholipid vesicles (spherosome)
title_sort	suppression of melanoma growth in a murine tumour model using orthosiphon stamineus benth. extract loaded in ethanolic phospholipid vesicles (spherosome)
publisher	Bentham Science Publ Ltd
publishDate	2022
url	http://eprints.um.edu.my/41471/
_version_	1778161677152288768
score	13.211869

Suppression of melanoma growth in a murine tumour model using orthosiphon stamineus benth. extract loaded in ethanolic phospholipid vesicles (spherosome)

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