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Nanocellulose and natural deep eutectic solvent as potential biocatalyst system toward enzyme immobilization

This study reports the immobilization of Candida Rugosa lipase (CRL) onto nanocellulose (NC) extracted from almond shells using p-toluenesulfonic acid (PTSA) and sulfuric acid (ASS) with sugar-based natural deep eutectic solvent (NADES1a) as a biocatalyst system. The properties of both immobilized l...

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Main Authors: Putra, Sharifah Shahira Syed, Basirun, Wan Jefrey, Elgharbawy, Amal A. M., Hayyan, Adeeb, Hayyan, Maan, Mohammed, Mohammed Alhaji
Format: Article
Published: Elsevier 2022
Subjects:
QD Chemistry
Online Access:http://eprints.um.edu.my/41212/
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spelling my.um.eprints.412122023-09-13T08:03:10Z http://eprints.um.edu.my/41212/ Nanocellulose and natural deep eutectic solvent as potential biocatalyst system toward enzyme immobilization Putra, Sharifah Shahira Syed Basirun, Wan Jefrey Elgharbawy, Amal A. M. Hayyan, Adeeb Hayyan, Maan Mohammed, Mohammed Alhaji QD Chemistry This study reports the immobilization of Candida Rugosa lipase (CRL) onto nanocellulose (NC) extracted from almond shells using p-toluenesulfonic acid (PTSA) and sulfuric acid (ASS) with sugar-based natural deep eutectic solvent (NADES1a) as a biocatalyst system. The properties of both immobilized lipases were studied and compared to the free enzyme counterpart. Under optimized conditions (2 h, 40 degrees C and pH 7.0), the immobilized CRL-PTSA-NADES1a and CRL-ASS-NADES1a gave a maximum specific activity of 4.9 U mg(-1) and 6.57 U mg(-1), respectively, compared to the free CRL (4.52 U mg(-1)). Both immobilized CRL showed better thermal stability, high catalytic activity and reusability up to 7 consecutive cycles. The half-life of the immobilized lipase was 14 similar to 16 days greater than free lipase (27 days). The Brunauer-Emmett-Teller (BET) surface area of NC-ASS (20.76 m(2) g(-1)) is higher compared to NC-PTSA (4.81 m(2) g(-1)). The functional groups and morphology of the free and immobilized CRL were further determined by Fourier transformed infrared (FTIR) and scanning electron microscopy (SEM). These findings revealed that the immobilized CRL onto NCs and NADES1a as green materials and solvent, respectively had higher lipase immobilization efficiency and stability for the treatment of food contaminants in oils in order to satisfy increasing commercial demands in the oil industry. Elsevier 2022-08 Article PeerReviewed Putra, Sharifah Shahira Syed and Basirun, Wan Jefrey and Elgharbawy, Amal A. M. and Hayyan, Adeeb and Hayyan, Maan and Mohammed, Mohammed Alhaji (2022) Nanocellulose and natural deep eutectic solvent as potential biocatalyst system toward enzyme immobilization. Molecular Catalysis, 528. ISSN 2468-8231, DOI https://doi.org/10.1016/j.mcat.2022.112422 <https://doi.org/10.1016/j.mcat.2022.112422>. 10.1016/j.mcat.2022.112422
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QD Chemistry
spellingShingle QD Chemistry
Putra, Sharifah Shahira Syed
Basirun, Wan Jefrey
Elgharbawy, Amal A. M.
Hayyan, Adeeb
Hayyan, Maan
Mohammed, Mohammed Alhaji
Nanocellulose and natural deep eutectic solvent as potential biocatalyst system toward enzyme immobilization
description This study reports the immobilization of Candida Rugosa lipase (CRL) onto nanocellulose (NC) extracted from almond shells using p-toluenesulfonic acid (PTSA) and sulfuric acid (ASS) with sugar-based natural deep eutectic solvent (NADES1a) as a biocatalyst system. The properties of both immobilized lipases were studied and compared to the free enzyme counterpart. Under optimized conditions (2 h, 40 degrees C and pH 7.0), the immobilized CRL-PTSA-NADES1a and CRL-ASS-NADES1a gave a maximum specific activity of 4.9 U mg(-1) and 6.57 U mg(-1), respectively, compared to the free CRL (4.52 U mg(-1)). Both immobilized CRL showed better thermal stability, high catalytic activity and reusability up to 7 consecutive cycles. The half-life of the immobilized lipase was 14 similar to 16 days greater than free lipase (27 days). The Brunauer-Emmett-Teller (BET) surface area of NC-ASS (20.76 m(2) g(-1)) is higher compared to NC-PTSA (4.81 m(2) g(-1)). The functional groups and morphology of the free and immobilized CRL were further determined by Fourier transformed infrared (FTIR) and scanning electron microscopy (SEM). These findings revealed that the immobilized CRL onto NCs and NADES1a as green materials and solvent, respectively had higher lipase immobilization efficiency and stability for the treatment of food contaminants in oils in order to satisfy increasing commercial demands in the oil industry.
format Article
author Putra, Sharifah Shahira Syed
Basirun, Wan Jefrey
Elgharbawy, Amal A. M.
Hayyan, Adeeb
Hayyan, Maan
Mohammed, Mohammed Alhaji
author_facet Putra, Sharifah Shahira Syed
Basirun, Wan Jefrey
Elgharbawy, Amal A. M.
Hayyan, Adeeb
Hayyan, Maan
Mohammed, Mohammed Alhaji
author_sort Putra, Sharifah Shahira Syed
title Nanocellulose and natural deep eutectic solvent as potential biocatalyst system toward enzyme immobilization
title_short Nanocellulose and natural deep eutectic solvent as potential biocatalyst system toward enzyme immobilization
title_full Nanocellulose and natural deep eutectic solvent as potential biocatalyst system toward enzyme immobilization
title_fullStr Nanocellulose and natural deep eutectic solvent as potential biocatalyst system toward enzyme immobilization
title_full_unstemmed Nanocellulose and natural deep eutectic solvent as potential biocatalyst system toward enzyme immobilization
title_sort nanocellulose and natural deep eutectic solvent as potential biocatalyst system toward enzyme immobilization
publisher Elsevier
publishDate 2022
url http://eprints.um.edu.my/41212/
_version_ 1778161642098393088
score 13.211869

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