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Detection of histamine receptors on mouse oocytes and their involvement in fertilization potential

Histamine widely involves in local immune responses, physiological function in the gut, and acting as a neurotransmitter in the brain. Scientist also found the importance of histamine in the reproductive systems. The present study aimed to determine the existence of histamine receptor subtypes; H1R,...

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Main Authors: Yusoff, Maisarah, Hashim, Noor Hashida, Mohd-Yusuf, Yusmin
Format: Article
Published: Urmia Univ 2022
Subjects:
QL Zoology
Online Access:http://eprints.um.edu.my/40982/
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spelling my.um.eprints.409822023-08-29T01:57:05Z http://eprints.um.edu.my/40982/ Detection of histamine receptors on mouse oocytes and their involvement in fertilization potential Yusoff, Maisarah Hashim, Noor Hashida Mohd-Yusuf, Yusmin QL Zoology Histamine widely involves in local immune responses, physiological function in the gut, and acting as a neurotransmitter in the brain. Scientist also found the importance of histamine in the reproductive systems. The present study aimed to determine the existence of histamine receptor subtypes; H1R, H2R, H3R, and H4R on mouse oocytes through immunofluorescence (IF) staining and reverse transcription- polymerase chain reaction (RT-PCR). These further confirmed by the involvement of histamine receptor antagonists in in vitro fertilization (IVF). In IF staining, mouse oocytes were incubated with primary antibody against histamine receptor, followed by incubation with fluorescence conjugated secondary antibody. Then RT-PCR analysis was carried out for the undetected receptors during IF for confirmation. The RT-PCR used RNA extracted from mice COCs and cumulus free oocytes. In IVF, sperm was cultured in a group of treated histamine receptor antagonists oocytes. This investigation revealed the existance of H1R, H2R, and H3R on mouse oocytes in IF and RT-PCR analyses. The treatment of IVF with histamine receptor antagonists (H1R: pyrilamine; H2R: cimetidine; H3R: thioperamide) led to a significant reduction quantity of 2-cell embryos (4.61 +/- 2.44%; 5.83 +/- 4.65%; 3.83 +/- 1.82%, respectively) as compared with the control group (22.50 +/- 6.44%). Therefore, according to the results of this study, the presence of H1R, H2R, and H3R on mouse oocytes possibly will suggest the involvement of histamine in fertilization. (c) 2022 Urmia University. All rights reserved. Urmia Univ 2022 Article PeerReviewed Yusoff, Maisarah and Hashim, Noor Hashida and Mohd-Yusuf, Yusmin (2022) Detection of histamine receptors on mouse oocytes and their involvement in fertilization potential. Veterinary Research Forum, 13 (3). pp. 331-337. ISSN 2008-8140, DOI https://doi.org/10.30466/vrf.2021.136028.3050 <https://doi.org/10.30466/vrf.2021.136028.3050>. 10.30466/vrf.2021.136028.3050
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QL Zoology
spellingShingle QL Zoology
Yusoff, Maisarah
Hashim, Noor Hashida
Mohd-Yusuf, Yusmin
Detection of histamine receptors on mouse oocytes and their involvement in fertilization potential
description Histamine widely involves in local immune responses, physiological function in the gut, and acting as a neurotransmitter in the brain. Scientist also found the importance of histamine in the reproductive systems. The present study aimed to determine the existence of histamine receptor subtypes; H1R, H2R, H3R, and H4R on mouse oocytes through immunofluorescence (IF) staining and reverse transcription- polymerase chain reaction (RT-PCR). These further confirmed by the involvement of histamine receptor antagonists in in vitro fertilization (IVF). In IF staining, mouse oocytes were incubated with primary antibody against histamine receptor, followed by incubation with fluorescence conjugated secondary antibody. Then RT-PCR analysis was carried out for the undetected receptors during IF for confirmation. The RT-PCR used RNA extracted from mice COCs and cumulus free oocytes. In IVF, sperm was cultured in a group of treated histamine receptor antagonists oocytes. This investigation revealed the existance of H1R, H2R, and H3R on mouse oocytes in IF and RT-PCR analyses. The treatment of IVF with histamine receptor antagonists (H1R: pyrilamine; H2R: cimetidine; H3R: thioperamide) led to a significant reduction quantity of 2-cell embryos (4.61 +/- 2.44%; 5.83 +/- 4.65%; 3.83 +/- 1.82%, respectively) as compared with the control group (22.50 +/- 6.44%). Therefore, according to the results of this study, the presence of H1R, H2R, and H3R on mouse oocytes possibly will suggest the involvement of histamine in fertilization. (c) 2022 Urmia University. All rights reserved.
format Article
author Yusoff, Maisarah
Hashim, Noor Hashida
Mohd-Yusuf, Yusmin
author_facet Yusoff, Maisarah
Hashim, Noor Hashida
Mohd-Yusuf, Yusmin
author_sort Yusoff, Maisarah
title Detection of histamine receptors on mouse oocytes and their involvement in fertilization potential
title_short Detection of histamine receptors on mouse oocytes and their involvement in fertilization potential
title_full Detection of histamine receptors on mouse oocytes and their involvement in fertilization potential
title_fullStr Detection of histamine receptors on mouse oocytes and their involvement in fertilization potential
title_full_unstemmed Detection of histamine receptors on mouse oocytes and their involvement in fertilization potential
title_sort detection of histamine receptors on mouse oocytes and their involvement in fertilization potential
publisher Urmia Univ
publishDate 2022
url http://eprints.um.edu.my/40982/
_version_ 1776247426276917248
score 13.211869

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