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Temperature and pH profiling of extracellular amylase from Antarctic and Arctic soil microfungi

While diversity studies and screening for enzyme activities are important elements of understanding fungal roles in the soil ecosystem, extracting and purifying the target enzyme from the fungal cellular system is also required to characterize the enzyme. This is, in particular, necessary before dev...

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Main Authors: Krishnan, Abiramy, Alias, Zazali, Convey, Peter, Gonzalez-Aravena, Marcelo, Smykla, Jerzy, Idid, Mohammed Rizman, Alias, Siti Aisah
Format: Article
Published: MDPI 2022
Subjects:
GE Environmental Sciences
QH301 Biology
Online Access:http://eprints.um.edu.my/40744/
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spelling my.um.eprints.407442023-11-10T08:33:00Z http://eprints.um.edu.my/40744/ Temperature and pH profiling of extracellular amylase from Antarctic and Arctic soil microfungi Krishnan, Abiramy Alias, Zazali Convey, Peter Gonzalez-Aravena, Marcelo Smykla, Jerzy Idid, Mohammed Rizman Alias, Siti Aisah GE Environmental Sciences QH301 Biology While diversity studies and screening for enzyme activities are important elements of understanding fungal roles in the soil ecosystem, extracting and purifying the target enzyme from the fungal cellular system is also required to characterize the enzyme. This is, in particular, necessary before developing the enzyme for industrial-scale production. In the present study, partially purified alpha-amylase was obtained from strains of Pseudogymnoascus sp. obtained from Antarctic and Arctic locations. Partially purified alpha-amylases from these polar fungi exhibited very similar characteristics, including being active at 15 degrees C, although having a small difference in optimum pH. Both fungal taxa are good candidates for the potential application of cold-active enzymes in biotechnological industries, and further purification and characterization steps are now required. The alpha-amylases from polar fungi are attractive in terms of industrial development because they are active at lower temperatures and acidic pH, thus potentially creating energy and cost savings. Furthermore, they prevent the production of maltulose, which is an undesirable by-product often formed under alkaline conditions. Psychrophilic amylases from the polar Pseudogymnoascus sp. investigated in the present study could provide a valuable future contribution to biotechnological applications. MDPI 2022-11 Article PeerReviewed Krishnan, Abiramy and Alias, Zazali and Convey, Peter and Gonzalez-Aravena, Marcelo and Smykla, Jerzy and Idid, Mohammed Rizman and Alias, Siti Aisah (2022) Temperature and pH profiling of extracellular amylase from Antarctic and Arctic soil microfungi. Fermentation-Basel, 8 (11). ISSN 2311-5637, DOI https://doi.org/10.3390/fermentation8110601 <https://doi.org/10.3390/fermentation8110601>. 10.3390/fermentation8110601
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic GE Environmental Sciences
QH301 Biology
spellingShingle GE Environmental Sciences
QH301 Biology
Krishnan, Abiramy
Alias, Zazali
Convey, Peter
Gonzalez-Aravena, Marcelo
Smykla, Jerzy
Idid, Mohammed Rizman
Alias, Siti Aisah
Temperature and pH profiling of extracellular amylase from Antarctic and Arctic soil microfungi
description While diversity studies and screening for enzyme activities are important elements of understanding fungal roles in the soil ecosystem, extracting and purifying the target enzyme from the fungal cellular system is also required to characterize the enzyme. This is, in particular, necessary before developing the enzyme for industrial-scale production. In the present study, partially purified alpha-amylase was obtained from strains of Pseudogymnoascus sp. obtained from Antarctic and Arctic locations. Partially purified alpha-amylases from these polar fungi exhibited very similar characteristics, including being active at 15 degrees C, although having a small difference in optimum pH. Both fungal taxa are good candidates for the potential application of cold-active enzymes in biotechnological industries, and further purification and characterization steps are now required. The alpha-amylases from polar fungi are attractive in terms of industrial development because they are active at lower temperatures and acidic pH, thus potentially creating energy and cost savings. Furthermore, they prevent the production of maltulose, which is an undesirable by-product often formed under alkaline conditions. Psychrophilic amylases from the polar Pseudogymnoascus sp. investigated in the present study could provide a valuable future contribution to biotechnological applications.
format Article
author Krishnan, Abiramy
Alias, Zazali
Convey, Peter
Gonzalez-Aravena, Marcelo
Smykla, Jerzy
Idid, Mohammed Rizman
Alias, Siti Aisah
author_facet Krishnan, Abiramy
Alias, Zazali
Convey, Peter
Gonzalez-Aravena, Marcelo
Smykla, Jerzy
Idid, Mohammed Rizman
Alias, Siti Aisah
author_sort Krishnan, Abiramy
title Temperature and pH profiling of extracellular amylase from Antarctic and Arctic soil microfungi
title_short Temperature and pH profiling of extracellular amylase from Antarctic and Arctic soil microfungi
title_full Temperature and pH profiling of extracellular amylase from Antarctic and Arctic soil microfungi
title_fullStr Temperature and pH profiling of extracellular amylase from Antarctic and Arctic soil microfungi
title_full_unstemmed Temperature and pH profiling of extracellular amylase from Antarctic and Arctic soil microfungi
title_sort temperature and ph profiling of extracellular amylase from antarctic and arctic soil microfungi
publisher MDPI
publishDate 2022
url http://eprints.um.edu.my/40744/
_version_ 1783876712456519680
score 13.211869

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