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Differentiating Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii using nested polymerase chain reaction (PCR) in rural communities in Malaysia

Background: In this study, a total of 426 human faecal samples were examined for the presence of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii infection via a combination of microscopic examination and nested polymerase chain reaction (PCR) targeting 16S ribosomal RNA of Entamoeba s...

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Main Authors: Ngui, Romano, Angal, Lorainne, Fakhrurrazi, Siti Aminah, Lim, Yvonne Ai Lian, Lau, Yee Ling, Ibrahim, Jamaiah, Mahmud, Rohela
Format: Article
Language:English
Published: BMC 2012
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/4052/1/1756-3305-5-187.pdf
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spelling my.um.eprints.40522019-08-26T04:25:04Z http://eprints.um.edu.my/4052/ Differentiating Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii using nested polymerase chain reaction (PCR) in rural communities in Malaysia Ngui, Romano Angal, Lorainne Fakhrurrazi, Siti Aminah Lim, Yvonne Ai Lian Lau, Yee Ling Ibrahim, Jamaiah Mahmud, Rohela R Medicine Background: In this study, a total of 426 human faecal samples were examined for the presence of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii infection via a combination of microscopic examination and nested polymerase chain reaction (PCR) targeting 16S ribosomal RNA of Entamoeba species. Methods: Faecal sample were collected from 426 participants in five rural villages in Peninsular Malaysia. The faecal samples were processed by direct wet smear and formalin ethyl acetate concentration technique followed by iodine staining and examined via microscopy for the presence of Entamoeba species and other intestinal parasites. Microscopically positive samples for Entamoeba species cysts were further characterized using a Nested Polymerase Chain Reaction (Nested-PCR) targeting 16S-like ribosomal RNA gene. The data entry and analysis was carried out using the SPSS software (Statistical Package for the Social Sciences) program for Windows version 17 (SPSS, Chicago, IL, USA). Results: Based on single faecal examination, overall prevalence of Entamoeba infection was 17.6 (75/426). Females (19.1) were more commonly infected compared to males (15.9). Comparison by age groups showed that adults (23.9) had higher infection rates than children (15.3). The PCR results showed that 52 out of 75 microscopy positive samples successfully generated species-specific amplicons. The infection with E. histolytica (75.0; 39/52) was the most common, followed by E. dispar (30.8; 18/52) and E. moshkovskii (5.8; 3/52). Of these, 33 (63.5) were shown to contain only E. histolytica, 10 (19.2) contained E. dispar and 3 (5.8) contained only E. moshkovskii. Mixed infection with E. histolytica and E. dispar was found in 6 (11.5) samples. The present study essentially emphasized the benefit of molecular techniques in discriminating the pathogenic Entamoeba species from the non-pathogenic for accurate diagnosis and better management of amoebiasis. The presence of E. moshkovskii is of great public health concern as it was the first time it has been reported in Malaysia. BMC 2012 Article PeerReviewed text en http://eprints.um.edu.my/4052/1/1756-3305-5-187.pdf Ngui, Romano and Angal, Lorainne and Fakhrurrazi, Siti Aminah and Lim, Yvonne Ai Lian and Lau, Yee Ling and Ibrahim, Jamaiah and Mahmud, Rohela (2012) Differentiating Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii using nested polymerase chain reaction (PCR) in rural communities in Malaysia. Parasites & Vectors, 5. p. 187. ISSN 1756-3305
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic R Medicine
spellingShingle R Medicine
Ngui, Romano
Angal, Lorainne
Fakhrurrazi, Siti Aminah
Lim, Yvonne Ai Lian
Lau, Yee Ling
Ibrahim, Jamaiah
Mahmud, Rohela
Differentiating Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii using nested polymerase chain reaction (PCR) in rural communities in Malaysia
description Background: In this study, a total of 426 human faecal samples were examined for the presence of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii infection via a combination of microscopic examination and nested polymerase chain reaction (PCR) targeting 16S ribosomal RNA of Entamoeba species. Methods: Faecal sample were collected from 426 participants in five rural villages in Peninsular Malaysia. The faecal samples were processed by direct wet smear and formalin ethyl acetate concentration technique followed by iodine staining and examined via microscopy for the presence of Entamoeba species and other intestinal parasites. Microscopically positive samples for Entamoeba species cysts were further characterized using a Nested Polymerase Chain Reaction (Nested-PCR) targeting 16S-like ribosomal RNA gene. The data entry and analysis was carried out using the SPSS software (Statistical Package for the Social Sciences) program for Windows version 17 (SPSS, Chicago, IL, USA). Results: Based on single faecal examination, overall prevalence of Entamoeba infection was 17.6 (75/426). Females (19.1) were more commonly infected compared to males (15.9). Comparison by age groups showed that adults (23.9) had higher infection rates than children (15.3). The PCR results showed that 52 out of 75 microscopy positive samples successfully generated species-specific amplicons. The infection with E. histolytica (75.0; 39/52) was the most common, followed by E. dispar (30.8; 18/52) and E. moshkovskii (5.8; 3/52). Of these, 33 (63.5) were shown to contain only E. histolytica, 10 (19.2) contained E. dispar and 3 (5.8) contained only E. moshkovskii. Mixed infection with E. histolytica and E. dispar was found in 6 (11.5) samples. The present study essentially emphasized the benefit of molecular techniques in discriminating the pathogenic Entamoeba species from the non-pathogenic for accurate diagnosis and better management of amoebiasis. The presence of E. moshkovskii is of great public health concern as it was the first time it has been reported in Malaysia.
format Article
author Ngui, Romano
Angal, Lorainne
Fakhrurrazi, Siti Aminah
Lim, Yvonne Ai Lian
Lau, Yee Ling
Ibrahim, Jamaiah
Mahmud, Rohela
author_facet Ngui, Romano
Angal, Lorainne
Fakhrurrazi, Siti Aminah
Lim, Yvonne Ai Lian
Lau, Yee Ling
Ibrahim, Jamaiah
Mahmud, Rohela
author_sort Ngui, Romano
title Differentiating Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii using nested polymerase chain reaction (PCR) in rural communities in Malaysia
title_short Differentiating Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii using nested polymerase chain reaction (PCR) in rural communities in Malaysia
title_full Differentiating Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii using nested polymerase chain reaction (PCR) in rural communities in Malaysia
title_fullStr Differentiating Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii using nested polymerase chain reaction (PCR) in rural communities in Malaysia
title_full_unstemmed Differentiating Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii using nested polymerase chain reaction (PCR) in rural communities in Malaysia
title_sort differentiating entamoeba histolytica, entamoeba dispar and entamoeba moshkovskii using nested polymerase chain reaction (pcr) in rural communities in malaysia
publisher BMC
publishDate 2012
url http://eprints.um.edu.my/4052/1/1756-3305-5-187.pdf
http://eprints.um.edu.my/4052/
_version_ 1643687250901336064
score 13.211869

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