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Predominance of Blastocystis sp subtype 4 in rural communities, Nepal

Blastocystis sp. is a common intestinal parasite. To date, there have been sporadic and scanty studies on Blastocystis sp. carried out in rural communities in Nepal. We surveyed the prevalence of Blastocystis sp. and its possible associated risk factors, and reported the predominant Blastocystis sp....

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Main Authors: Lee, I.L., Tan, T.C., Tan, P.C., Nanthiney, D.R., Biraj, M.K., Surendra, K.M., Suresh, K.G.
Format: Article
Language:English
Published: 2012
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/4030/1/Lee-2012-Predominance_of_Blas.pdf
http://eprints.um.edu.my/4030/
http://www.springerlink.com/content/k78455466h83025v/fulltext.pdf
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spelling my.um.eprints.40302014-12-16T04:33:24Z http://eprints.um.edu.my/4030/ Predominance of Blastocystis sp subtype 4 in rural communities, Nepal Lee, I.L. Tan, T.C. Tan, P.C. Nanthiney, D.R. Biraj, M.K. Surendra, K.M. Suresh, K.G. R Medicine Blastocystis sp. is a common intestinal parasite. To date, there have been sporadic and scanty studies on Blastocystis sp. carried out in rural communities in Nepal. We surveyed the prevalence of Blastocystis sp. and its possible associated risk factors, and reported the predominant Blastocystis sp. subtype in two rural communities, Bolde Phediche and Bahunipati, in Nepal. Human faecal samples were collected from 241 participants, cultured using in vitro cultivation and examined for Blastocystis sp. The presence of Blastocystis sp. in faecal samples was further confirmed by polymerase chain reaction (PCR) and subsequently genotyped using subtype-specific sequence tagged site (STS) primers. There were 26.1 (63/241) of the participants that were infected by Blastocystis sp. We detected 84.1 (53/63) of Blastocystis sp. subtype 4 infections in these rural communities. The unusually high prevalence of Blastocystis sp. subtype 4 can be attributed to the rearing of family-owned animals in barns built close to their houses. Eighty one percent (51/63) of the Blastocystis sp. infected participants drank not boiled or unfiltered water. The present study revealed that Blastocystis sp. could pose a health concern to the communities and travellers to the hilly area in Nepal. Infection may be transmitted through human-to-human, zoonotic and waterborne transmissions. We provide recommendations to ensure good public health practices. 2012 Article PeerReviewed application/pdf en http://eprints.um.edu.my/4030/1/Lee-2012-Predominance_of_Blas.pdf Lee, I.L. and Tan, T.C. and Tan, P.C. and Nanthiney, D.R. and Biraj, M.K. and Surendra, K.M. and Suresh, K.G. (2012) Predominance of Blastocystis sp subtype 4 in rural communities, Nepal. Parasitology Research, 110 (4). pp. 1553-1562. ISSN 0932-0113 http://www.springerlink.com/content/k78455466h83025v/fulltext.pdf 10.1007/s00436-011-2665-0
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic R Medicine
spellingShingle R Medicine
Lee, I.L.
Tan, T.C.
Tan, P.C.
Nanthiney, D.R.
Biraj, M.K.
Surendra, K.M.
Suresh, K.G.
Predominance of Blastocystis sp subtype 4 in rural communities, Nepal
description Blastocystis sp. is a common intestinal parasite. To date, there have been sporadic and scanty studies on Blastocystis sp. carried out in rural communities in Nepal. We surveyed the prevalence of Blastocystis sp. and its possible associated risk factors, and reported the predominant Blastocystis sp. subtype in two rural communities, Bolde Phediche and Bahunipati, in Nepal. Human faecal samples were collected from 241 participants, cultured using in vitro cultivation and examined for Blastocystis sp. The presence of Blastocystis sp. in faecal samples was further confirmed by polymerase chain reaction (PCR) and subsequently genotyped using subtype-specific sequence tagged site (STS) primers. There were 26.1 (63/241) of the participants that were infected by Blastocystis sp. We detected 84.1 (53/63) of Blastocystis sp. subtype 4 infections in these rural communities. The unusually high prevalence of Blastocystis sp. subtype 4 can be attributed to the rearing of family-owned animals in barns built close to their houses. Eighty one percent (51/63) of the Blastocystis sp. infected participants drank not boiled or unfiltered water. The present study revealed that Blastocystis sp. could pose a health concern to the communities and travellers to the hilly area in Nepal. Infection may be transmitted through human-to-human, zoonotic and waterborne transmissions. We provide recommendations to ensure good public health practices.
format Article
author Lee, I.L.
Tan, T.C.
Tan, P.C.
Nanthiney, D.R.
Biraj, M.K.
Surendra, K.M.
Suresh, K.G.
author_facet Lee, I.L.
Tan, T.C.
Tan, P.C.
Nanthiney, D.R.
Biraj, M.K.
Surendra, K.M.
Suresh, K.G.
author_sort Lee, I.L.
title Predominance of Blastocystis sp subtype 4 in rural communities, Nepal
title_short Predominance of Blastocystis sp subtype 4 in rural communities, Nepal
title_full Predominance of Blastocystis sp subtype 4 in rural communities, Nepal
title_fullStr Predominance of Blastocystis sp subtype 4 in rural communities, Nepal
title_full_unstemmed Predominance of Blastocystis sp subtype 4 in rural communities, Nepal
title_sort predominance of blastocystis sp subtype 4 in rural communities, nepal
publishDate 2012
url http://eprints.um.edu.my/4030/1/Lee-2012-Predominance_of_Blas.pdf
http://eprints.um.edu.my/4030/
http://www.springerlink.com/content/k78455466h83025v/fulltext.pdf
_version_ 1643687245259997184
score 13.211869

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