Staff View: Cardiovascular biomarker troponin I biosensor: Aptamer-gold-antibody hybrid on a metal oxide surface

Cardiovascular biomarker troponin I biosensor: Aptamer-gold-antibody hybrid on a metal oxide surface

Myocardial infarction (MI) is highly related to cardiac arrest leading to death and organ damage. Radiological techniques and electrocardiography have been used as preliminary tests to diagnose MI; however, these techniques are not sensitive enough for early-stage detection. A blood biomarker-based...

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Main Authors:	Hui, Hu, Gopinath, Subash C. B., Ismail, Zool H., Chen, Yeng, Pandian, K., Velusamy, Palaniyandi
Format:	Article
Published:	Wiley 2023
Subjects:	Q Science (General) QD Chemistry QH301 Biology
Online Access:	http://eprints.um.edu.my/39507/
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spelling	my.um.eprints.395072023-07-13T03:21:49Z http://eprints.um.edu.my/39507/ Cardiovascular biomarker troponin I biosensor: Aptamer-gold-antibody hybrid on a metal oxide surface Hui, Hu Gopinath, Subash C. B. Ismail, Zool H. Chen, Yeng Pandian, K. Velusamy, Palaniyandi Q Science (General) QD Chemistry QH301 Biology Myocardial infarction (MI) is highly related to cardiac arrest leading to death and organ damage. Radiological techniques and electrocardiography have been used as preliminary tests to diagnose MI; however, these techniques are not sensitive enough for early-stage detection. A blood biomarker-based diagnosis is an immediate solution, and due to the high correlation of troponin with MI, it has been considered to be a gold-standard biomarker. In the present research, the cardiac biomarker troponin I (cTnI) was detected on an interdigitated electrode sensor with various surface interfaces. To detect cTnI, a capture aptamer-conjugated gold nanoparticle probe and detection antibody probe were utilized and compared through an alternating sandwich pattern. The surface metal oxide morphology of the developed sensor was proven by microscopic assessments. The limit of detection with the aptamer-gold-cTnI-antibody sandwich pattern was 100 aM, while it was 1 fM with antibody-gold-cTnI-aptamer, representing 10-fold differences. Further, the high performance of the sensor was confirmed by selective cTnI determination in serum, exhibiting superior nonfouling. These methods of determination provide options for generating novel assays for diagnosing MI. Wiley 2023-04 Article PeerReviewed Hui, Hu and Gopinath, Subash C. B. and Ismail, Zool H. and Chen, Yeng and Pandian, K. and Velusamy, Palaniyandi (2023) Cardiovascular biomarker troponin I biosensor: Aptamer-gold-antibody hybrid on a metal oxide surface. Biotechnology and Applied Biochemistry, 70 (2). pp. 581-591. ISSN 0885-4513, DOI https://doi.org/10.1002/bab.2380 <https://doi.org/10.1002/bab.2380>. 10.1002/bab.2380
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	Q Science (General) QD Chemistry QH301 Biology
spellingShingle	Q Science (General) QD Chemistry QH301 Biology Hui, Hu Gopinath, Subash C. B. Ismail, Zool H. Chen, Yeng Pandian, K. Velusamy, Palaniyandi Cardiovascular biomarker troponin I biosensor: Aptamer-gold-antibody hybrid on a metal oxide surface
description	Myocardial infarction (MI) is highly related to cardiac arrest leading to death and organ damage. Radiological techniques and electrocardiography have been used as preliminary tests to diagnose MI; however, these techniques are not sensitive enough for early-stage detection. A blood biomarker-based diagnosis is an immediate solution, and due to the high correlation of troponin with MI, it has been considered to be a gold-standard biomarker. In the present research, the cardiac biomarker troponin I (cTnI) was detected on an interdigitated electrode sensor with various surface interfaces. To detect cTnI, a capture aptamer-conjugated gold nanoparticle probe and detection antibody probe were utilized and compared through an alternating sandwich pattern. The surface metal oxide morphology of the developed sensor was proven by microscopic assessments. The limit of detection with the aptamer-gold-cTnI-antibody sandwich pattern was 100 aM, while it was 1 fM with antibody-gold-cTnI-aptamer, representing 10-fold differences. Further, the high performance of the sensor was confirmed by selective cTnI determination in serum, exhibiting superior nonfouling. These methods of determination provide options for generating novel assays for diagnosing MI.
format	Article
author	Hui, Hu Gopinath, Subash C. B. Ismail, Zool H. Chen, Yeng Pandian, K. Velusamy, Palaniyandi
author_facet	Hui, Hu Gopinath, Subash C. B. Ismail, Zool H. Chen, Yeng Pandian, K. Velusamy, Palaniyandi
author_sort	Hui, Hu
title	Cardiovascular biomarker troponin I biosensor: Aptamer-gold-antibody hybrid on a metal oxide surface
title_short	Cardiovascular biomarker troponin I biosensor: Aptamer-gold-antibody hybrid on a metal oxide surface
title_full	Cardiovascular biomarker troponin I biosensor: Aptamer-gold-antibody hybrid on a metal oxide surface
title_fullStr	Cardiovascular biomarker troponin I biosensor: Aptamer-gold-antibody hybrid on a metal oxide surface
title_full_unstemmed	Cardiovascular biomarker troponin I biosensor: Aptamer-gold-antibody hybrid on a metal oxide surface
title_sort	cardiovascular biomarker troponin i biosensor: aptamer-gold-antibody hybrid on a metal oxide surface
publisher	Wiley
publishDate	2023
url	http://eprints.um.edu.my/39507/
_version_	1772811759361458176
score	13.211869

Cardiovascular biomarker troponin I biosensor: Aptamer-gold-antibody hybrid on a metal oxide surface

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