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Genetic polymorphism of the thrombospondin-related apical merozoite protein (TRAMP) of Plasmodium knowlesi in Malaysia

Plasmodium knowlesi is a simian malaria parasite that causes significant zoonotic infections in Southeast Asia, particularly in Malaysia. The Plasmodium thrombospondin-related apical merozoite protein (TRAMP) plays an essential role in the invasion of the parasite into its host erythrocyte. The pres...

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Main Authors: Ng, Yee Ling, Lau, Yee Ling, Hamid, Mohd Hafizi Abdul, Jelip, Jenarun, Ooi, Choo Huck, Mudin, Rose Nani, Jaimin, Joel Judson, Fong, Mun Yik
Format: Article
Published: Springer 2023
Subjects:
QH426 Genetics
Online Access:http://eprints.um.edu.my/39328/
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spelling my.um.eprints.393282023-11-29T01:16:21Z http://eprints.um.edu.my/39328/ Genetic polymorphism of the thrombospondin-related apical merozoite protein (TRAMP) of Plasmodium knowlesi in Malaysia Ng, Yee Ling Lau, Yee Ling Hamid, Mohd Hafizi Abdul Jelip, Jenarun Ooi, Choo Huck Mudin, Rose Nani Jaimin, Joel Judson Fong, Mun Yik QH426 Genetics Plasmodium knowlesi is a simian malaria parasite that causes significant zoonotic infections in Southeast Asia, particularly in Malaysia. The Plasmodium thrombospondin-related apical merozoite protein (TRAMP) plays an essential role in the invasion of the parasite into its host erythrocyte. The present study investigated the genetic polymorphism and natural selection of the full length PkTRAMP from P. knowlesi clinical isolates from Malaysia. Blood samples (n=40) were collected from P. knowlesi malaria patients from Peninsular Malaysia and Malaysian Borneo. The PkTRAMP gene was amplified using PCR, followed by cloning into a plasmid vector and sequenced. Results showed that the nucleotide diversity of PkTRAMP was low (pi: 0.009). Z-test results indicated negative (purifying) selection of PkTRAMP. The alignment of the deduced amino acid sequences of PkTRAMP of Peninsular Malaysia and Malaysian Borneo revealed 38 dimorphic sites. A total of 27 haplotypes were identified from the amino acid sequence alignment. Haplotype analysis revealed that there was no clustering of PkTRAMP from Peninsular Malaysia and Malaysian Borneo. Springer 2023-01 Article PeerReviewed Ng, Yee Ling and Lau, Yee Ling and Hamid, Mohd Hafizi Abdul and Jelip, Jenarun and Ooi, Choo Huck and Mudin, Rose Nani and Jaimin, Joel Judson and Fong, Mun Yik (2023) Genetic polymorphism of the thrombospondin-related apical merozoite protein (TRAMP) of Plasmodium knowlesi in Malaysia. Parasitology Research, 122 (1). pp. 195-200. ISSN 0932-0113, DOI https://doi.org/10.1007/s00436-022-07716-z <https://doi.org/10.1007/s00436-022-07716-z>. 10.1007/s00436-022-07716-z
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QH426 Genetics
spellingShingle QH426 Genetics
Ng, Yee Ling
Lau, Yee Ling
Hamid, Mohd Hafizi Abdul
Jelip, Jenarun
Ooi, Choo Huck
Mudin, Rose Nani
Jaimin, Joel Judson
Fong, Mun Yik
Genetic polymorphism of the thrombospondin-related apical merozoite protein (TRAMP) of Plasmodium knowlesi in Malaysia
description Plasmodium knowlesi is a simian malaria parasite that causes significant zoonotic infections in Southeast Asia, particularly in Malaysia. The Plasmodium thrombospondin-related apical merozoite protein (TRAMP) plays an essential role in the invasion of the parasite into its host erythrocyte. The present study investigated the genetic polymorphism and natural selection of the full length PkTRAMP from P. knowlesi clinical isolates from Malaysia. Blood samples (n=40) were collected from P. knowlesi malaria patients from Peninsular Malaysia and Malaysian Borneo. The PkTRAMP gene was amplified using PCR, followed by cloning into a plasmid vector and sequenced. Results showed that the nucleotide diversity of PkTRAMP was low (pi: 0.009). Z-test results indicated negative (purifying) selection of PkTRAMP. The alignment of the deduced amino acid sequences of PkTRAMP of Peninsular Malaysia and Malaysian Borneo revealed 38 dimorphic sites. A total of 27 haplotypes were identified from the amino acid sequence alignment. Haplotype analysis revealed that there was no clustering of PkTRAMP from Peninsular Malaysia and Malaysian Borneo.
format Article
author Ng, Yee Ling
Lau, Yee Ling
Hamid, Mohd Hafizi Abdul
Jelip, Jenarun
Ooi, Choo Huck
Mudin, Rose Nani
Jaimin, Joel Judson
Fong, Mun Yik
author_facet Ng, Yee Ling
Lau, Yee Ling
Hamid, Mohd Hafizi Abdul
Jelip, Jenarun
Ooi, Choo Huck
Mudin, Rose Nani
Jaimin, Joel Judson
Fong, Mun Yik
author_sort Ng, Yee Ling
title Genetic polymorphism of the thrombospondin-related apical merozoite protein (TRAMP) of Plasmodium knowlesi in Malaysia
title_short Genetic polymorphism of the thrombospondin-related apical merozoite protein (TRAMP) of Plasmodium knowlesi in Malaysia
title_full Genetic polymorphism of the thrombospondin-related apical merozoite protein (TRAMP) of Plasmodium knowlesi in Malaysia
title_fullStr Genetic polymorphism of the thrombospondin-related apical merozoite protein (TRAMP) of Plasmodium knowlesi in Malaysia
title_full_unstemmed Genetic polymorphism of the thrombospondin-related apical merozoite protein (TRAMP) of Plasmodium knowlesi in Malaysia
title_sort genetic polymorphism of the thrombospondin-related apical merozoite protein (tramp) of plasmodium knowlesi in malaysia
publisher Springer
publishDate 2023
url http://eprints.um.edu.my/39328/
_version_ 1783876684637798400
score 13.211869

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