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Antifungal activity of secondary metabolites purified from Bacillus subtilis isolated in Vietnam and evaluated on in vitro and in vivo models

The supernatant from Bacillus subtilis possessed significant antifungal activity against Fusarium oxysporum and Rhizoctonia solani, two harmful pathogenic fungi. In this study, antifungal compounds were purified from the extracellular extract of B. subtilis including volatile organic compounds (VOCs...

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Main Authors:	Tuyen, Do Thi, Trung, Nguyen Thi, Thao, Nguyen Thi, Thanh, Nguyen Sy Le, Nguyen, Nguyen Phuong Dai, Tuyet, Nguyen Thi Anh, Cuong, Nguyen Tien, Chan, Sook Sin, Khoo, Kuan Shiong, Show, Pau Loke
Format:	Article
Published:	Elsevier 2023
Subjects:	Q Science (General) QR Microbiology T Technology (General)
Online Access:	http://eprints.um.edu.my/38866/
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spelling	my.um.eprints.388662023-06-28T03:06:55Z http://eprints.um.edu.my/38866/ Antifungal activity of secondary metabolites purified from Bacillus subtilis isolated in Vietnam and evaluated on in vitro and in vivo models Tuyen, Do Thi Trung, Nguyen Thi Thao, Nguyen Thi Thanh, Nguyen Sy Le Nguyen, Nguyen Phuong Dai Tuyet, Nguyen Thi Anh Cuong, Nguyen Tien Chan, Sook Sin Khoo, Kuan Shiong Show, Pau Loke Q Science (General) QR Microbiology T Technology (General) The supernatant from Bacillus subtilis possessed significant antifungal activity against Fusarium oxysporum and Rhizoctonia solani, two harmful pathogenic fungi. In this study, antifungal compounds were purified from the extracellular extract of B. subtilis including volatile organic compounds (VOCs) and membranes protein. The antifungal compound was purified from B. subtilis by using a silica gel column with a solvent system of chlo-roform: ethyl acetate (7:3, v/v). Based on spectral data, the chemical structure was identified as (3R,4R)-and (3S,4S)-3.4-dihydroxyl-3-methyl-2-pentanone (acetylbutanediol)-volatile organic compounds. The 3.4-dihy-droxyl-3-methyl-2-pentanone showed high antifungal activity against Fusarium oxysporum and Rhizoctonia sol-ani. Additionally, we isolated a novel antifungal protein with a molecular mass of 40 kDa. The results of Matrix -assisted laser desorption/ionization-Time of flight (MALDI-TOF) mass suggested that the functional molecule was outer membrane proteins (OMPs). The purified secondary metabolites retained antifungal activity against R. solani after being treated at 100 degrees C for 30 min. Treatment with the crude broth produced from Bacillus subtilis highly inhibited the growth of tested pathogens. Hence, it exhibited the potential of the antifungal compounds from B. subtilis for use as a biocontrol agent against fungi. Elsevier 2023-04 Article PeerReviewed Tuyen, Do Thi and Trung, Nguyen Thi and Thao, Nguyen Thi and Thanh, Nguyen Sy Le and Nguyen, Nguyen Phuong Dai and Tuyet, Nguyen Thi Anh and Cuong, Nguyen Tien and Chan, Sook Sin and Khoo, Kuan Shiong and Show, Pau Loke (2023) Antifungal activity of secondary metabolites purified from Bacillus subtilis isolated in Vietnam and evaluated on in vitro and in vivo models. International Biodeterioration and Biodegradation, 179. ISSN 0964-8305, DOI https://doi.org/10.1016/j.ibiod.2022.105558 <https://doi.org/10.1016/j.ibiod.2022.105558>. 10.1016/j.ibiod.2022.105558
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	Q Science (General) QR Microbiology T Technology (General)
spellingShingle	Q Science (General) QR Microbiology T Technology (General) Tuyen, Do Thi Trung, Nguyen Thi Thao, Nguyen Thi Thanh, Nguyen Sy Le Nguyen, Nguyen Phuong Dai Tuyet, Nguyen Thi Anh Cuong, Nguyen Tien Chan, Sook Sin Khoo, Kuan Shiong Show, Pau Loke Antifungal activity of secondary metabolites purified from Bacillus subtilis isolated in Vietnam and evaluated on in vitro and in vivo models
description	The supernatant from Bacillus subtilis possessed significant antifungal activity against Fusarium oxysporum and Rhizoctonia solani, two harmful pathogenic fungi. In this study, antifungal compounds were purified from the extracellular extract of B. subtilis including volatile organic compounds (VOCs) and membranes protein. The antifungal compound was purified from B. subtilis by using a silica gel column with a solvent system of chlo-roform: ethyl acetate (7:3, v/v). Based on spectral data, the chemical structure was identified as (3R,4R)-and (3S,4S)-3.4-dihydroxyl-3-methyl-2-pentanone (acetylbutanediol)-volatile organic compounds. The 3.4-dihy-droxyl-3-methyl-2-pentanone showed high antifungal activity against Fusarium oxysporum and Rhizoctonia sol-ani. Additionally, we isolated a novel antifungal protein with a molecular mass of 40 kDa. The results of Matrix -assisted laser desorption/ionization-Time of flight (MALDI-TOF) mass suggested that the functional molecule was outer membrane proteins (OMPs). The purified secondary metabolites retained antifungal activity against R. solani after being treated at 100 degrees C for 30 min. Treatment with the crude broth produced from Bacillus subtilis highly inhibited the growth of tested pathogens. Hence, it exhibited the potential of the antifungal compounds from B. subtilis for use as a biocontrol agent against fungi.
format	Article
author	Tuyen, Do Thi Trung, Nguyen Thi Thao, Nguyen Thi Thanh, Nguyen Sy Le Nguyen, Nguyen Phuong Dai Tuyet, Nguyen Thi Anh Cuong, Nguyen Tien Chan, Sook Sin Khoo, Kuan Shiong Show, Pau Loke
author_facet	Tuyen, Do Thi Trung, Nguyen Thi Thao, Nguyen Thi Thanh, Nguyen Sy Le Nguyen, Nguyen Phuong Dai Tuyet, Nguyen Thi Anh Cuong, Nguyen Tien Chan, Sook Sin Khoo, Kuan Shiong Show, Pau Loke
author_sort	Tuyen, Do Thi
title	Antifungal activity of secondary metabolites purified from Bacillus subtilis isolated in Vietnam and evaluated on in vitro and in vivo models
title_short	Antifungal activity of secondary metabolites purified from Bacillus subtilis isolated in Vietnam and evaluated on in vitro and in vivo models
title_full	Antifungal activity of secondary metabolites purified from Bacillus subtilis isolated in Vietnam and evaluated on in vitro and in vivo models
title_fullStr	Antifungal activity of secondary metabolites purified from Bacillus subtilis isolated in Vietnam and evaluated on in vitro and in vivo models
title_full_unstemmed	Antifungal activity of secondary metabolites purified from Bacillus subtilis isolated in Vietnam and evaluated on in vitro and in vivo models
title_sort	antifungal activity of secondary metabolites purified from bacillus subtilis isolated in vietnam and evaluated on in vitro and in vivo models
publisher	Elsevier
publishDate	2023
url	http://eprints.um.edu.my/38866/
_version_	1770551496010629120
score	13.211869

Antifungal activity of secondary metabolites purified from Bacillus subtilis isolated in Vietnam and evaluated on in vitro and in vivo models

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