Staff View: Molecular surveillance for vector-borne bacteria in rodents and tree shrews of Peninsular Malaysia oil palm plantations

Molecular surveillance for vector-borne bacteria in rodents and tree shrews of Peninsular Malaysia oil palm plantations

Many human clinical cases attributed to vector-borne pathogens are underreported in Malaysia, especially in rural localities where healthcare infrastructures are lacking. Here, 217 small mammals, consisting of rodents and tree shrews, were trapped in oil palm plantations in the Peninsular Malaysia s...

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Main Authors:	Mohd-Azami, Siti Nurul Izzah, Loong, Shih Keng, Khoo, Jing Jing, Husin, Nurul Aini, Lim, Fang Shiang, Mahfodz, Nur Hidayana, Ishak, Siti Nabilah, Mohd-Taib, Farah Shafawati, Makepeace, Benjamin L. L., Abu Bakar, Sazaly
Format:	Article
Published:	MDPI 2023
Subjects:	R Medicine (General) S Agriculture (General)
Online Access:	http://eprints.um.edu.my/38720/
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spelling	my.um.eprints.387202023-12-01T03:23:49Z http://eprints.um.edu.my/38720/ Molecular surveillance for vector-borne bacteria in rodents and tree shrews of Peninsular Malaysia oil palm plantations Mohd-Azami, Siti Nurul Izzah Loong, Shih Keng Khoo, Jing Jing Husin, Nurul Aini Lim, Fang Shiang Mahfodz, Nur Hidayana Ishak, Siti Nabilah Mohd-Taib, Farah Shafawati Makepeace, Benjamin L. L. Abu Bakar, Sazaly R Medicine (General) S Agriculture (General) Many human clinical cases attributed to vector-borne pathogens are underreported in Malaysia, especially in rural localities where healthcare infrastructures are lacking. Here, 217 small mammals, consisting of rodents and tree shrews, were trapped in oil palm plantations in the Peninsular Malaysia states of Johor and Perak. Species identification was performed using morphological and DNA barcoding analyses, and 203 small mammals were included in the detection of selected vector-borne bacteria. The DNA extracted from the spleens was examined for Orientia tsutsugamushi, Borrelia spp., Bartonella spp. and Rickettsia spp. using established PCR assays. The small mammals collected in this study included Rattus tanezumi R3 mitotype (n = 113), Rattus argentiventer (n = 24), Rattus tiomanicus (n = 22), Rattus exulans (n = 17), Rattus tanezumi sensu stricto (n = 1) and Tupaia glis (n = 40). Orientia tsutsugamushi, Borrelia spp. and Bartonella phoceensis were detected in the small mammals with the respective detection rates of 12.3%, 5.9% and 4.9%. Rickettsia spp., however, was not detected. This study encountered the presence of both Lyme disease and relapsing fever-related borreliae in small mammals collected from the oil palm plantation study sites. All three microorganisms (Orientia tsutsugamushi, Borrelia spp. and Bartonella phoceensis) were detected in the R. tanezumi R3 mitotype, suggesting that the species is a competent host for multiple microorganisms. Further investigations are warranted to elucidate the relationships between the ectoparasites, the small mammals and the respective pathogens. MDPI 2023-02 Article PeerReviewed Mohd-Azami, Siti Nurul Izzah and Loong, Shih Keng and Khoo, Jing Jing and Husin, Nurul Aini and Lim, Fang Shiang and Mahfodz, Nur Hidayana and Ishak, Siti Nabilah and Mohd-Taib, Farah Shafawati and Makepeace, Benjamin L. L. and Abu Bakar, Sazaly (2023) Molecular surveillance for vector-borne bacteria in rodents and tree shrews of Peninsular Malaysia oil palm plantations. Tropical Medicine and Infectious Disease, 8 (2). ISSN 2414-6366, DOI https://doi.org/10.3390/tropicalmed8020074 <https://doi.org/10.3390/tropicalmed8020074>. 10.3390/tropicalmed8020074
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	R Medicine (General) S Agriculture (General)
spellingShingle	R Medicine (General) S Agriculture (General) Mohd-Azami, Siti Nurul Izzah Loong, Shih Keng Khoo, Jing Jing Husin, Nurul Aini Lim, Fang Shiang Mahfodz, Nur Hidayana Ishak, Siti Nabilah Mohd-Taib, Farah Shafawati Makepeace, Benjamin L. L. Abu Bakar, Sazaly Molecular surveillance for vector-borne bacteria in rodents and tree shrews of Peninsular Malaysia oil palm plantations
description	Many human clinical cases attributed to vector-borne pathogens are underreported in Malaysia, especially in rural localities where healthcare infrastructures are lacking. Here, 217 small mammals, consisting of rodents and tree shrews, were trapped in oil palm plantations in the Peninsular Malaysia states of Johor and Perak. Species identification was performed using morphological and DNA barcoding analyses, and 203 small mammals were included in the detection of selected vector-borne bacteria. The DNA extracted from the spleens was examined for Orientia tsutsugamushi, Borrelia spp., Bartonella spp. and Rickettsia spp. using established PCR assays. The small mammals collected in this study included Rattus tanezumi R3 mitotype (n = 113), Rattus argentiventer (n = 24), Rattus tiomanicus (n = 22), Rattus exulans (n = 17), Rattus tanezumi sensu stricto (n = 1) and Tupaia glis (n = 40). Orientia tsutsugamushi, Borrelia spp. and Bartonella phoceensis were detected in the small mammals with the respective detection rates of 12.3%, 5.9% and 4.9%. Rickettsia spp., however, was not detected. This study encountered the presence of both Lyme disease and relapsing fever-related borreliae in small mammals collected from the oil palm plantation study sites. All three microorganisms (Orientia tsutsugamushi, Borrelia spp. and Bartonella phoceensis) were detected in the R. tanezumi R3 mitotype, suggesting that the species is a competent host for multiple microorganisms. Further investigations are warranted to elucidate the relationships between the ectoparasites, the small mammals and the respective pathogens.
format	Article
author	Mohd-Azami, Siti Nurul Izzah Loong, Shih Keng Khoo, Jing Jing Husin, Nurul Aini Lim, Fang Shiang Mahfodz, Nur Hidayana Ishak, Siti Nabilah Mohd-Taib, Farah Shafawati Makepeace, Benjamin L. L. Abu Bakar, Sazaly
author_facet	Mohd-Azami, Siti Nurul Izzah Loong, Shih Keng Khoo, Jing Jing Husin, Nurul Aini Lim, Fang Shiang Mahfodz, Nur Hidayana Ishak, Siti Nabilah Mohd-Taib, Farah Shafawati Makepeace, Benjamin L. L. Abu Bakar, Sazaly
author_sort	Mohd-Azami, Siti Nurul Izzah
title	Molecular surveillance for vector-borne bacteria in rodents and tree shrews of Peninsular Malaysia oil palm plantations
title_short	Molecular surveillance for vector-borne bacteria in rodents and tree shrews of Peninsular Malaysia oil palm plantations
title_full	Molecular surveillance for vector-borne bacteria in rodents and tree shrews of Peninsular Malaysia oil palm plantations
title_fullStr	Molecular surveillance for vector-borne bacteria in rodents and tree shrews of Peninsular Malaysia oil palm plantations
title_full_unstemmed	Molecular surveillance for vector-borne bacteria in rodents and tree shrews of Peninsular Malaysia oil palm plantations
title_sort	molecular surveillance for vector-borne bacteria in rodents and tree shrews of peninsular malaysia oil palm plantations
publisher	MDPI
publishDate	2023
url	http://eprints.um.edu.my/38720/
_version_	1784511847502708736
score	13.211869

Molecular surveillance for vector-borne bacteria in rodents and tree shrews of Peninsular Malaysia oil palm plantations

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