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Simultaneous detection of Methicillin-resistant staphylococcus aureus, acinetobacter baumanii, escherichia coli, klebsiella pneumoniae and pseudomonas aeruginosa by multiplex PCR

A PCR-based assay that can simultaneously detect and differentiate five different types of nosocomial bacterial pathogens was developed. Six pairs of selected primers targeting femA (132 bp) and mecA (310 bp) of methicillin-resistant Staphylococcus aureus, gltA (722 bp) of Acinetobacter baumannii, p...

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Main Authors: Thong, Kwai Lin, Lai, M.Y., Teh, C.S.J., Chua, K.H.
Format: Article
Published: Malaysian Society of Parasitology and Tropical Medicine 2011
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/3848/
http://www.msptm.org/files/21_-_31_Thong_KL.pdf
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spelling my.um.eprints.38482018-10-23T05:44:45Z http://eprints.um.edu.my/3848/ Simultaneous detection of Methicillin-resistant staphylococcus aureus, acinetobacter baumanii, escherichia coli, klebsiella pneumoniae and pseudomonas aeruginosa by multiplex PCR Thong, Kwai Lin Lai, M.Y. Teh, C.S.J. Chua, K.H. R Medicine A PCR-based assay that can simultaneously detect and differentiate five different types of nosocomial bacterial pathogens was developed. Six pairs of selected primers targeting femA (132 bp) and mecA (310 bp) of methicillin-resistant Staphylococcus aureus, gltA (722 bp) of Acinetobacter baumannii, phoA (903 bp) of Escherichia coli, mdh (364 bp) of Klebsiella pneumoniae and oprL (504 bp) of Pseudomonas aeruginosa were used in this study. The conditions were optimized for the multiplex PCR to ensure specific amplification of the selected targets. Sensitivity and specificity tests were also carried out using a blind test approach on 50 bacterial cultures and resulted in 100 for both positive and negative predictive values. Malaysian Society of Parasitology and Tropical Medicine 2011 Article PeerReviewed Thong, Kwai Lin and Lai, M.Y. and Teh, C.S.J. and Chua, K.H. (2011) Simultaneous detection of Methicillin-resistant staphylococcus aureus, acinetobacter baumanii, escherichia coli, klebsiella pneumoniae and pseudomonas aeruginosa by multiplex PCR. Tropical Biomedicine, 28 (1). pp. 21-31. ISSN 0127-5720 http://www.msptm.org/files/21_-_31_Thong_KL.pdf
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Thong, Kwai Lin
Lai, M.Y.
Teh, C.S.J.
Chua, K.H.
Simultaneous detection of Methicillin-resistant staphylococcus aureus, acinetobacter baumanii, escherichia coli, klebsiella pneumoniae and pseudomonas aeruginosa by multiplex PCR
description A PCR-based assay that can simultaneously detect and differentiate five different types of nosocomial bacterial pathogens was developed. Six pairs of selected primers targeting femA (132 bp) and mecA (310 bp) of methicillin-resistant Staphylococcus aureus, gltA (722 bp) of Acinetobacter baumannii, phoA (903 bp) of Escherichia coli, mdh (364 bp) of Klebsiella pneumoniae and oprL (504 bp) of Pseudomonas aeruginosa were used in this study. The conditions were optimized for the multiplex PCR to ensure specific amplification of the selected targets. Sensitivity and specificity tests were also carried out using a blind test approach on 50 bacterial cultures and resulted in 100 for both positive and negative predictive values.
format Article
author Thong, Kwai Lin
Lai, M.Y.
Teh, C.S.J.
Chua, K.H.
author_facet Thong, Kwai Lin
Lai, M.Y.
Teh, C.S.J.
Chua, K.H.
author_sort Thong, Kwai Lin
title Simultaneous detection of Methicillin-resistant staphylococcus aureus, acinetobacter baumanii, escherichia coli, klebsiella pneumoniae and pseudomonas aeruginosa by multiplex PCR
title_short Simultaneous detection of Methicillin-resistant staphylococcus aureus, acinetobacter baumanii, escherichia coli, klebsiella pneumoniae and pseudomonas aeruginosa by multiplex PCR
title_full Simultaneous detection of Methicillin-resistant staphylococcus aureus, acinetobacter baumanii, escherichia coli, klebsiella pneumoniae and pseudomonas aeruginosa by multiplex PCR
title_fullStr Simultaneous detection of Methicillin-resistant staphylococcus aureus, acinetobacter baumanii, escherichia coli, klebsiella pneumoniae and pseudomonas aeruginosa by multiplex PCR
title_full_unstemmed Simultaneous detection of Methicillin-resistant staphylococcus aureus, acinetobacter baumanii, escherichia coli, klebsiella pneumoniae and pseudomonas aeruginosa by multiplex PCR
title_sort simultaneous detection of methicillin-resistant staphylococcus aureus, acinetobacter baumanii, escherichia coli, klebsiella pneumoniae and pseudomonas aeruginosa by multiplex pcr
publisher Malaysian Society of Parasitology and Tropical Medicine
publishDate 2011
url http://eprints.um.edu.my/3848/
http://www.msptm.org/files/21_-_31_Thong_KL.pdf
_version_ 1643687211115216896
score 13.211869

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