Staff View: Proteomic analysis, immuno-specificity and neutralization efficacy of Pakistani viper antivenom (pvav), a bivalent anti-viperid antivenom produced in Pakistan

Proteomic analysis, immuno-specificity and neutralization efficacy of Pakistani viper antivenom (pvav), a bivalent anti-viperid antivenom produced in Pakistan

Snakebite envenoming is a neglected tropical disease prevalent in South Asia. In Pakistan, antivenoms are commonly imported from India despite the controversy over their effectiveness. To solve the problem, the locals have developed the Pakistani Viper Antivenom (PVAV), raised against Sochurek'...

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Main Authors:	Lim, Andy Shing Seng, Tan, Kae Yi, Quraishi, Naeem H., Farooque, Saud, Khoso, Zahoor Ahmed, Ratanabanangkoon, Kavi, Tan, Choo Hock
Format:	Article
Published:	MDPI 2023
Subjects:	RS Pharmacy and materia medica T Technology (General)
Online Access:	http://eprints.um.edu.my/38348/
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spelling	my.um.eprints.383482024-11-27T03:35:07Z http://eprints.um.edu.my/38348/ Proteomic analysis, immuno-specificity and neutralization efficacy of Pakistani viper antivenom (pvav), a bivalent anti-viperid antivenom produced in Pakistan Lim, Andy Shing Seng Tan, Kae Yi Quraishi, Naeem H. Farooque, Saud Khoso, Zahoor Ahmed Ratanabanangkoon, Kavi Tan, Choo Hock RS Pharmacy and materia medica T Technology (General) Snakebite envenoming is a neglected tropical disease prevalent in South Asia. In Pakistan, antivenoms are commonly imported from India despite the controversy over their effectiveness. To solve the problem, the locals have developed the Pakistani Viper Antivenom (PVAV), raised against Sochurek's Saw-scaled Viper (Echis carinatus sochureki) and Russell's Viper (Daboia russelii) of Pakistani origin. This study is set to evaluate the composition purity, immuno-specificity and neutralization efficacy of PVAV. Chromatographic and electrophoretic profiling coupled with proteomic mass spectrometry analysis showed PVAV containing high-purity immunoglobulin G with minimum impurities, notably the absence of serum albumin. PVAV is highly immuno-specific toward the venoms of the two vipers and Echis carinatus multisquamatus, which are indigenous to Pakistan. Its immunoreactivity, however, reduces toward the venoms of other Echis carinatus subspecies and D. russelii from South India as well as Sri Lanka. Meanwhile, its non-specific binding activities for the venoms of Hump-nosed Pit Vipers, Indian Cobras and kraits were extremely low. In the neutralization study, PVAV effectively mitigated the hemotoxic and lethal effects of the Pakistani viper venoms, tested in vitro and in vivo. Together, the findings suggest the potential utility of PVAV as a new domestic antivenom for the treatment of viperid envenoming in Pakistan. MDPI 2023-04 Article PeerReviewed Lim, Andy Shing Seng and Tan, Kae Yi and Quraishi, Naeem H. and Farooque, Saud and Khoso, Zahoor Ahmed and Ratanabanangkoon, Kavi and Tan, Choo Hock (2023) Proteomic analysis, immuno-specificity and neutralization efficacy of Pakistani viper antivenom (pvav), a bivalent anti-viperid antivenom produced in Pakistan. Toxins, 15 (4). ISSN 2072-6651, DOI https://doi.org/10.3390/toxins15040265 <https://doi.org/10.3390/toxins15040265>. 10.3390/toxins15040265
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	RS Pharmacy and materia medica T Technology (General)
spellingShingle	RS Pharmacy and materia medica T Technology (General) Lim, Andy Shing Seng Tan, Kae Yi Quraishi, Naeem H. Farooque, Saud Khoso, Zahoor Ahmed Ratanabanangkoon, Kavi Tan, Choo Hock Proteomic analysis, immuno-specificity and neutralization efficacy of Pakistani viper antivenom (pvav), a bivalent anti-viperid antivenom produced in Pakistan
description	Snakebite envenoming is a neglected tropical disease prevalent in South Asia. In Pakistan, antivenoms are commonly imported from India despite the controversy over their effectiveness. To solve the problem, the locals have developed the Pakistani Viper Antivenom (PVAV), raised against Sochurek's Saw-scaled Viper (Echis carinatus sochureki) and Russell's Viper (Daboia russelii) of Pakistani origin. This study is set to evaluate the composition purity, immuno-specificity and neutralization efficacy of PVAV. Chromatographic and electrophoretic profiling coupled with proteomic mass spectrometry analysis showed PVAV containing high-purity immunoglobulin G with minimum impurities, notably the absence of serum albumin. PVAV is highly immuno-specific toward the venoms of the two vipers and Echis carinatus multisquamatus, which are indigenous to Pakistan. Its immunoreactivity, however, reduces toward the venoms of other Echis carinatus subspecies and D. russelii from South India as well as Sri Lanka. Meanwhile, its non-specific binding activities for the venoms of Hump-nosed Pit Vipers, Indian Cobras and kraits were extremely low. In the neutralization study, PVAV effectively mitigated the hemotoxic and lethal effects of the Pakistani viper venoms, tested in vitro and in vivo. Together, the findings suggest the potential utility of PVAV as a new domestic antivenom for the treatment of viperid envenoming in Pakistan.
format	Article
author	Lim, Andy Shing Seng Tan, Kae Yi Quraishi, Naeem H. Farooque, Saud Khoso, Zahoor Ahmed Ratanabanangkoon, Kavi Tan, Choo Hock
author_facet	Lim, Andy Shing Seng Tan, Kae Yi Quraishi, Naeem H. Farooque, Saud Khoso, Zahoor Ahmed Ratanabanangkoon, Kavi Tan, Choo Hock
author_sort	Lim, Andy Shing Seng
title	Proteomic analysis, immuno-specificity and neutralization efficacy of Pakistani viper antivenom (pvav), a bivalent anti-viperid antivenom produced in Pakistan
title_short	Proteomic analysis, immuno-specificity and neutralization efficacy of Pakistani viper antivenom (pvav), a bivalent anti-viperid antivenom produced in Pakistan
title_full	Proteomic analysis, immuno-specificity and neutralization efficacy of Pakistani viper antivenom (pvav), a bivalent anti-viperid antivenom produced in Pakistan
title_fullStr	Proteomic analysis, immuno-specificity and neutralization efficacy of Pakistani viper antivenom (pvav), a bivalent anti-viperid antivenom produced in Pakistan
title_full_unstemmed	Proteomic analysis, immuno-specificity and neutralization efficacy of Pakistani viper antivenom (pvav), a bivalent anti-viperid antivenom produced in Pakistan
title_sort	proteomic analysis, immuno-specificity and neutralization efficacy of pakistani viper antivenom (pvav), a bivalent anti-viperid antivenom produced in pakistan
publisher	MDPI
publishDate	2023
url	http://eprints.um.edu.my/38348/
_version_	1817841934716108800
score	13.222552

Proteomic analysis, immuno-specificity and neutralization efficacy of Pakistani viper antivenom (pvav), a bivalent anti-viperid antivenom produced in Pakistan

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