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Further evaluation of a multiplex PCR for differentiation of Salmonella paratyphi A from other salmonellae

Salmonella enterica serovar Paratyphi A is a causative agent of paratyphoid fever. The clinical syndrome caused by paratyphoid fever overlaps with other febrile illnesses and cannot be distinguished from typhoid fever. Conventional methods used for diagnosis are time consuming, costly, and labor-int...

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Main Authors: Teh, C.S., Chua, Kek Heng, Puthucheary, S.D., Thong, Kwai Lin
Format: Article
Language:English
Published: Institute of Infectious Diseases 2008
Subjects:
Q Science (General)
QH Natural history
R Medicine
Online Access:http://eprints.um.edu.my/3810/1/Further_Evaluation_of_a_Multiplex_PCR_for_differentiation_of_Salmonella_Paratyphi_A_from_other_Salmonellae..pdf
http://eprints.um.edu.my/3810/
http://www0.nih.go.jp/JJID/61/313.pdf
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spelling my.um.eprints.38102019-03-21T04:43:05Z http://eprints.um.edu.my/3810/ Further evaluation of a multiplex PCR for differentiation of Salmonella paratyphi A from other salmonellae Teh, C.S. Chua, Kek Heng Puthucheary, S.D. Thong, Kwai Lin Q Science (General) QH Natural history R Medicine Salmonella enterica serovar Paratyphi A is a causative agent of paratyphoid fever. The clinical syndrome caused by paratyphoid fever overlaps with other febrile illnesses and cannot be distinguished from typhoid fever. Conventional methods used for diagnosis are time consuming, costly, and labor-intensive. We evaluated the specificity, sensitivity, and application of a multiplex polymerase chain reaction (PCR) previously developed by the method (Ou, H.Y., Teh, C.S.J., Thong, K.L., et al., J. Mol. Diagn., 9, 624-630, 2007) using 6 S. Paratyphi A, 22 S. Typhi, and 85 other Salmonella serovars as well as 36 non-Salmonella strains. The detection limit of the multiplex PCR was 4 x 10(4) cfu ml(-1). In a blind test of the other 50 strains, this multiplex PCR correctly identified the only S. Paratyphi A in the panel of strains. The sensitivity of this PCR using spiked blood and stool samples was 1 x 10(5) cfu ml(-1) and 2 x 10(5) cfu ml(-1), respectively, but increased to 1 x 10(4) cfu ml(-1) and 2 x 10(3) cfu ml(-1) after 5-h enrichment. We believe that this multiplex PCR is a promising technique for the specific and sensitive detection of S. Paratyphi A in clinical, environmental, and food samples. Institute of Infectious Diseases 2008 Article PeerReviewed application/pdf en http://eprints.um.edu.my/3810/1/Further_Evaluation_of_a_Multiplex_PCR_for_differentiation_of_Salmonella_Paratyphi_A_from_other_Salmonellae..pdf Teh, C.S. and Chua, Kek Heng and Puthucheary, S.D. and Thong, Kwai Lin (2008) Further evaluation of a multiplex PCR for differentiation of Salmonella paratyphi A from other salmonellae. Japanese Journal of Infectious Diseases, 61 (4). pp. 313-314. ISSN 1344-6304 http://www0.nih.go.jp/JJID/61/313.pdf
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic Q Science (General)
QH Natural history
R Medicine
spellingShingle Q Science (General)
QH Natural history
R Medicine
Teh, C.S.
Chua, Kek Heng
Puthucheary, S.D.
Thong, Kwai Lin
Further evaluation of a multiplex PCR for differentiation of Salmonella paratyphi A from other salmonellae
description Salmonella enterica serovar Paratyphi A is a causative agent of paratyphoid fever. The clinical syndrome caused by paratyphoid fever overlaps with other febrile illnesses and cannot be distinguished from typhoid fever. Conventional methods used for diagnosis are time consuming, costly, and labor-intensive. We evaluated the specificity, sensitivity, and application of a multiplex polymerase chain reaction (PCR) previously developed by the method (Ou, H.Y., Teh, C.S.J., Thong, K.L., et al., J. Mol. Diagn., 9, 624-630, 2007) using 6 S. Paratyphi A, 22 S. Typhi, and 85 other Salmonella serovars as well as 36 non-Salmonella strains. The detection limit of the multiplex PCR was 4 x 10(4) cfu ml(-1). In a blind test of the other 50 strains, this multiplex PCR correctly identified the only S. Paratyphi A in the panel of strains. The sensitivity of this PCR using spiked blood and stool samples was 1 x 10(5) cfu ml(-1) and 2 x 10(5) cfu ml(-1), respectively, but increased to 1 x 10(4) cfu ml(-1) and 2 x 10(3) cfu ml(-1) after 5-h enrichment. We believe that this multiplex PCR is a promising technique for the specific and sensitive detection of S. Paratyphi A in clinical, environmental, and food samples.
format Article
author Teh, C.S.
Chua, Kek Heng
Puthucheary, S.D.
Thong, Kwai Lin
author_facet Teh, C.S.
Chua, Kek Heng
Puthucheary, S.D.
Thong, Kwai Lin
author_sort Teh, C.S.
title Further evaluation of a multiplex PCR for differentiation of Salmonella paratyphi A from other salmonellae
title_short Further evaluation of a multiplex PCR for differentiation of Salmonella paratyphi A from other salmonellae
title_full Further evaluation of a multiplex PCR for differentiation of Salmonella paratyphi A from other salmonellae
title_fullStr Further evaluation of a multiplex PCR for differentiation of Salmonella paratyphi A from other salmonellae
title_full_unstemmed Further evaluation of a multiplex PCR for differentiation of Salmonella paratyphi A from other salmonellae
title_sort further evaluation of a multiplex pcr for differentiation of salmonella paratyphi a from other salmonellae
publisher Institute of Infectious Diseases
publishDate 2008
url http://eprints.um.edu.my/3810/1/Further_Evaluation_of_a_Multiplex_PCR_for_differentiation_of_Salmonella_Paratyphi_A_from_other_Salmonellae..pdf
http://eprints.um.edu.my/3810/
http://www0.nih.go.jp/JJID/61/313.pdf
_version_ 1643687203923034112
score 13.211869

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