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Adaptive features of in vitro-derived plantlets of MD2 pineapple during acclimatization process

Tissue culture-derived planting materials require optimal acclimatization period to ensure high survival rates in the nursery and to save labor and production cost. However, there is little information on how the morphology and physiology of in vitro-derived plantlets can be affected by culture cond...

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Main Authors:	Hamid, Nur Syazwani, Tan, Boon Chin, Khalid, Norzulaani, Osman, Normaniza, Jalil, Mahanom
Format:	Article
Published:	Horticultural Society of India 2020
Subjects:	Q Science (General) QH301 Biology S Agriculture (General)
Online Access:	http://eprints.um.edu.my/37106/
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spelling	my.um.eprints.371062023-05-30T06:39:34Z http://eprints.um.edu.my/37106/ Adaptive features of in vitro-derived plantlets of MD2 pineapple during acclimatization process Hamid, Nur Syazwani Tan, Boon Chin Khalid, Norzulaani Osman, Normaniza Jalil, Mahanom Q Science (General) QH301 Biology S Agriculture (General) Tissue culture-derived planting materials require optimal acclimatization period to ensure high survival rates in the nursery and to save labor and production cost. However, there is little information on how the morphology and physiology of in vitro-derived plantlets can be affected by culture conditions and acclimatization. We compared different types of media for shoot multiplication using thirty pineapple suckers as explants. Morphological and physiological changes of thirty field-grown plants and in vitro-derived plantlets during ex vitro acclimatization at 0, 14, 28, 42, 56 and 84 days were evaluated. Experiments were conducted in triplicate. Observations were based on the leaf morphology (number, width, ratio value of dry weight to fresh weight and succulence index of the leaves) and physiological characteristics (photosynthetic rate, stomatal conductance, transpiration rate, stomata opening, trichomes and histological examination). We found that the in vitro plantlets undergoing acclimatization showed C3 characteristics and adapted to CAM characteristics after 42 days of acclimatization. The in vitro plantlets were compared with field-grown plants and observations were made based on the morphological and physiological characteristics of the leaves. In conclusion, our findings indicated that in vitro-derived pineapple plantlets started to develop and achieve similar characteristics with the field-grown CAM plants after 42 days of acclimatization, suggesting that this could be the suitable period prior to transfer into the fields. Horticultural Society of India 2020-12 Article PeerReviewed Hamid, Nur Syazwani and Tan, Boon Chin and Khalid, Norzulaani and Osman, Normaniza and Jalil, Mahanom (2020) Adaptive features of in vitro-derived plantlets of MD2 pineapple during acclimatization process. Indian Journal of Horticulture, 77 (4). pp. 595-602. ISSN 0972-8538, DOI https://doi.org/10.5958/0974-0112.2020.00086.9 <https://doi.org/10.5958/0974-0112.2020.00086.9>. 10.5958/0974-0112.2020.00086.9
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	Q Science (General) QH301 Biology S Agriculture (General)
spellingShingle	Q Science (General) QH301 Biology S Agriculture (General) Hamid, Nur Syazwani Tan, Boon Chin Khalid, Norzulaani Osman, Normaniza Jalil, Mahanom Adaptive features of in vitro-derived plantlets of MD2 pineapple during acclimatization process
description	Tissue culture-derived planting materials require optimal acclimatization period to ensure high survival rates in the nursery and to save labor and production cost. However, there is little information on how the morphology and physiology of in vitro-derived plantlets can be affected by culture conditions and acclimatization. We compared different types of media for shoot multiplication using thirty pineapple suckers as explants. Morphological and physiological changes of thirty field-grown plants and in vitro-derived plantlets during ex vitro acclimatization at 0, 14, 28, 42, 56 and 84 days were evaluated. Experiments were conducted in triplicate. Observations were based on the leaf morphology (number, width, ratio value of dry weight to fresh weight and succulence index of the leaves) and physiological characteristics (photosynthetic rate, stomatal conductance, transpiration rate, stomata opening, trichomes and histological examination). We found that the in vitro plantlets undergoing acclimatization showed C3 characteristics and adapted to CAM characteristics after 42 days of acclimatization. The in vitro plantlets were compared with field-grown plants and observations were made based on the morphological and physiological characteristics of the leaves. In conclusion, our findings indicated that in vitro-derived pineapple plantlets started to develop and achieve similar characteristics with the field-grown CAM plants after 42 days of acclimatization, suggesting that this could be the suitable period prior to transfer into the fields.
format	Article
author	Hamid, Nur Syazwani Tan, Boon Chin Khalid, Norzulaani Osman, Normaniza Jalil, Mahanom
author_facet	Hamid, Nur Syazwani Tan, Boon Chin Khalid, Norzulaani Osman, Normaniza Jalil, Mahanom
author_sort	Hamid, Nur Syazwani
title	Adaptive features of in vitro-derived plantlets of MD2 pineapple during acclimatization process
title_short	Adaptive features of in vitro-derived plantlets of MD2 pineapple during acclimatization process
title_full	Adaptive features of in vitro-derived plantlets of MD2 pineapple during acclimatization process
title_fullStr	Adaptive features of in vitro-derived plantlets of MD2 pineapple during acclimatization process
title_full_unstemmed	Adaptive features of in vitro-derived plantlets of MD2 pineapple during acclimatization process
title_sort	adaptive features of in vitro-derived plantlets of md2 pineapple during acclimatization process
publisher	Horticultural Society of India
publishDate	2020
url	http://eprints.um.edu.my/37106/
_version_	1768007309661831168
score	13.211869

Adaptive features of in vitro-derived plantlets of MD2 pineapple during acclimatization process

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