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Fabrication of biopolymer polyhydroxyalkanoate/chitosan and 2D molybdenum disulfide-doped scaffolds for antibacterial and biomedical applications

Antibiotic resistance in pathogenic bacteria is a major health challenge, as Infectious Diseases Society of America (IDSA) has recognized that the past simply drugs susceptible pathogens are now the most dangerous pathogens due to their nonstop growing resistance towards conventional antibiotics. Th...

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Main Authors: Mukheem, Abdul, Shahabuddin, Syed, Akbar, Noor, Anwar, Ayaz, Sarih, Norazilawati Muhamad, Sudesh, Kumar, Khan, Naveed Ahmed, Sridewi, Nanthini
Format: Article
Published: Springer 2020
Subjects:
Q Science (General)
QD Chemistry
Online Access:http://eprints.um.edu.my/36883/
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spelling my.um.eprints.368832023-12-31T07:29:03Z http://eprints.um.edu.my/36883/ Fabrication of biopolymer polyhydroxyalkanoate/chitosan and 2D molybdenum disulfide-doped scaffolds for antibacterial and biomedical applications Mukheem, Abdul Shahabuddin, Syed Akbar, Noor Anwar, Ayaz Sarih, Norazilawati Muhamad Sudesh, Kumar Khan, Naveed Ahmed Sridewi, Nanthini Q Science (General) QD Chemistry Antibiotic resistance in pathogenic bacteria is a major health challenge, as Infectious Diseases Society of America (IDSA) has recognized that the past simply drugs susceptible pathogens are now the most dangerous pathogens due to their nonstop growing resistance towards conventional antibiotics. Therefore, due to the emergence of multi-drug resistance, the bacterial infections have become a serious global problem. Acute infections feasibly develop into chronic infections because of many factors; one of them is the failure of effectiveness of antibiotics against superbugs. Modern research of two-dimensional nanoparticles and biopolymers are of great interest to attain the intricate bactericidal activity. In this study, we fabricated an antibacterial nanocomposite consisting of representative two-dimensional molybdenum disulfide (2D MoS2) nanoparticles. Polyhydroxyalkanoate (PHA) and chitosan (Ch) are used to encapsulate MoS2 nanoparticles into their matrix. This study reports the in vitro antibacterial activity and host cytotoxicity of novel PHA-Ch/MoS2 nanocomposites. PHA-Ch/MoS2 nanocomposites were subjected to time-dependent antibacterial assays at various doses to examine their antibacterial activity against multi-drug-resistant Escherichia coli K1 (Malaysian Type Culture Collection 710859) and methicillin-resistant Staphylococcus aureus (MRSA) (Malaysian Type Culture Collection 381123). Furthermore, the cytotoxicity of nanocomposites was examined against spontaneously immortalized human keratinocyte (HaCaT) cell lines. The results indicated significant antibacterial activity (p value < 0.05) against E. coli K1 and MRSA. In addition, PHA-Ch/MoS2 showed significant host cytocompatibility (p < 0.05) against HaCaT cells. The fabricated PHA-Ch/MoS2 nanocomposites have demonstrated effective antibacterial activity against both Gram-positive and -negative bacteria and exhibited better biocompatibility. Finally, PHA-Ch/MoS2 nanocomposites are shown to be suitable for antibacterial applications and also hold potential for further biomedical studies. Springer 2020-04 Article PeerReviewed Mukheem, Abdul and Shahabuddin, Syed and Akbar, Noor and Anwar, Ayaz and Sarih, Norazilawati Muhamad and Sudesh, Kumar and Khan, Naveed Ahmed and Sridewi, Nanthini (2020) Fabrication of biopolymer polyhydroxyalkanoate/chitosan and 2D molybdenum disulfide-doped scaffolds for antibacterial and biomedical applications. Applied Microbiology and Biotechnology, 104 (7). pp. 3121-3131. ISSN 0175-7598, DOI https://doi.org/10.1007/s00253-020-10416-2 <https://doi.org/10.1007/s00253-020-10416-2>. 10.1007/s00253-020-10416-2
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic Q Science (General)
QD Chemistry
spellingShingle Q Science (General)
QD Chemistry
Mukheem, Abdul
Shahabuddin, Syed
Akbar, Noor
Anwar, Ayaz
Sarih, Norazilawati Muhamad
Sudesh, Kumar
Khan, Naveed Ahmed
Sridewi, Nanthini
Fabrication of biopolymer polyhydroxyalkanoate/chitosan and 2D molybdenum disulfide-doped scaffolds for antibacterial and biomedical applications
description Antibiotic resistance in pathogenic bacteria is a major health challenge, as Infectious Diseases Society of America (IDSA) has recognized that the past simply drugs susceptible pathogens are now the most dangerous pathogens due to their nonstop growing resistance towards conventional antibiotics. Therefore, due to the emergence of multi-drug resistance, the bacterial infections have become a serious global problem. Acute infections feasibly develop into chronic infections because of many factors; one of them is the failure of effectiveness of antibiotics against superbugs. Modern research of two-dimensional nanoparticles and biopolymers are of great interest to attain the intricate bactericidal activity. In this study, we fabricated an antibacterial nanocomposite consisting of representative two-dimensional molybdenum disulfide (2D MoS2) nanoparticles. Polyhydroxyalkanoate (PHA) and chitosan (Ch) are used to encapsulate MoS2 nanoparticles into their matrix. This study reports the in vitro antibacterial activity and host cytotoxicity of novel PHA-Ch/MoS2 nanocomposites. PHA-Ch/MoS2 nanocomposites were subjected to time-dependent antibacterial assays at various doses to examine their antibacterial activity against multi-drug-resistant Escherichia coli K1 (Malaysian Type Culture Collection 710859) and methicillin-resistant Staphylococcus aureus (MRSA) (Malaysian Type Culture Collection 381123). Furthermore, the cytotoxicity of nanocomposites was examined against spontaneously immortalized human keratinocyte (HaCaT) cell lines. The results indicated significant antibacterial activity (p value < 0.05) against E. coli K1 and MRSA. In addition, PHA-Ch/MoS2 showed significant host cytocompatibility (p < 0.05) against HaCaT cells. The fabricated PHA-Ch/MoS2 nanocomposites have demonstrated effective antibacterial activity against both Gram-positive and -negative bacteria and exhibited better biocompatibility. Finally, PHA-Ch/MoS2 nanocomposites are shown to be suitable for antibacterial applications and also hold potential for further biomedical studies.
format Article
author Mukheem, Abdul
Shahabuddin, Syed
Akbar, Noor
Anwar, Ayaz
Sarih, Norazilawati Muhamad
Sudesh, Kumar
Khan, Naveed Ahmed
Sridewi, Nanthini
author_facet Mukheem, Abdul
Shahabuddin, Syed
Akbar, Noor
Anwar, Ayaz
Sarih, Norazilawati Muhamad
Sudesh, Kumar
Khan, Naveed Ahmed
Sridewi, Nanthini
author_sort Mukheem, Abdul
title Fabrication of biopolymer polyhydroxyalkanoate/chitosan and 2D molybdenum disulfide-doped scaffolds for antibacterial and biomedical applications
title_short Fabrication of biopolymer polyhydroxyalkanoate/chitosan and 2D molybdenum disulfide-doped scaffolds for antibacterial and biomedical applications
title_full Fabrication of biopolymer polyhydroxyalkanoate/chitosan and 2D molybdenum disulfide-doped scaffolds for antibacterial and biomedical applications
title_fullStr Fabrication of biopolymer polyhydroxyalkanoate/chitosan and 2D molybdenum disulfide-doped scaffolds for antibacterial and biomedical applications
title_full_unstemmed Fabrication of biopolymer polyhydroxyalkanoate/chitosan and 2D molybdenum disulfide-doped scaffolds for antibacterial and biomedical applications
title_sort fabrication of biopolymer polyhydroxyalkanoate/chitosan and 2d molybdenum disulfide-doped scaffolds for antibacterial and biomedical applications
publisher Springer
publishDate 2020
url http://eprints.um.edu.my/36883/
_version_ 1787133828582604800
score 13.211869

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