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Evaluation of WarmStart Colorimetric Loop-Mediated Isothermal Amplification Assay for Diagnosis of Malaria

The incidence of zoonotic malaria, Plasmodium knowlesi, infection is increasing and now is the major cause of malaria in Malaysia. Here, we describe a WarmStart colorimetric loop-mediated isothermal amplification (LAMP) assay for the detection of Plasmodium spp. The detection limit for this assay wa...

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Main Authors: Lai, Meng Yee, Ooi, Choo Huck, Jaimin, Joel Judson, Lau, Yee Ling
Format: Article
Published: American Society of Tropical Medicine and Hygiene 2020
Subjects:
R Medicine
RA0421 Public health. Hygiene. Preventive Medicine
Online Access:http://eprints.um.edu.my/36657/
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spelling my.um.eprints.366572023-12-31T04:05:07Z http://eprints.um.edu.my/36657/ Evaluation of WarmStart Colorimetric Loop-Mediated Isothermal Amplification Assay for Diagnosis of Malaria Lai, Meng Yee Ooi, Choo Huck Jaimin, Joel Judson Lau, Yee Ling R Medicine RA0421 Public health. Hygiene. Preventive Medicine The incidence of zoonotic malaria, Plasmodium knowlesi, infection is increasing and now is the major cause of malaria in Malaysia. Here, we describe a WarmStart colorimetric loop-mediated isothermal amplification (LAMP) assay for the detection of Plasmodium spp. The detection limit for this assay was 10 copies/mu L for P knowlesi and Plasmodium ovale and 1 copy/mu L for Plasmodium falciparum, Plasmodium vivax, and Plasmodium malariae. To test clinical sensitivity and specificity, 100 microscopy-positive and 20 malaria-negative samples were used. The WarmStart colorimetric LAMP was 98% sensitive and 100% specific. Amplification products were visible for direct observation, thereby eliminating the need for post-amplification processing steps. Therefore, WarmStart colorimetric LAMP is suitable for use in resource-limited settings. American Society of Tropical Medicine and Hygiene 2020-06 Article PeerReviewed Lai, Meng Yee and Ooi, Choo Huck and Jaimin, Joel Judson and Lau, Yee Ling (2020) Evaluation of WarmStart Colorimetric Loop-Mediated Isothermal Amplification Assay for Diagnosis of Malaria. American Journal of Tropical Medicine and Hygiene, 102 (6). pp. 1370-1372. ISSN 0002-9637, DOI https://doi.org/10.4269/ajtmh.20-0001 <https://doi.org/10.4269/ajtmh.20-0001>. 10.4269/ajtmh.20-0001
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
RA0421 Public health. Hygiene. Preventive Medicine
spellingShingle R Medicine
RA0421 Public health. Hygiene. Preventive Medicine
Lai, Meng Yee
Ooi, Choo Huck
Jaimin, Joel Judson
Lau, Yee Ling
Evaluation of WarmStart Colorimetric Loop-Mediated Isothermal Amplification Assay for Diagnosis of Malaria
description The incidence of zoonotic malaria, Plasmodium knowlesi, infection is increasing and now is the major cause of malaria in Malaysia. Here, we describe a WarmStart colorimetric loop-mediated isothermal amplification (LAMP) assay for the detection of Plasmodium spp. The detection limit for this assay was 10 copies/mu L for P knowlesi and Plasmodium ovale and 1 copy/mu L for Plasmodium falciparum, Plasmodium vivax, and Plasmodium malariae. To test clinical sensitivity and specificity, 100 microscopy-positive and 20 malaria-negative samples were used. The WarmStart colorimetric LAMP was 98% sensitive and 100% specific. Amplification products were visible for direct observation, thereby eliminating the need for post-amplification processing steps. Therefore, WarmStart colorimetric LAMP is suitable for use in resource-limited settings.
format Article
author Lai, Meng Yee
Ooi, Choo Huck
Jaimin, Joel Judson
Lau, Yee Ling
author_facet Lai, Meng Yee
Ooi, Choo Huck
Jaimin, Joel Judson
Lau, Yee Ling
author_sort Lai, Meng Yee
title Evaluation of WarmStart Colorimetric Loop-Mediated Isothermal Amplification Assay for Diagnosis of Malaria
title_short Evaluation of WarmStart Colorimetric Loop-Mediated Isothermal Amplification Assay for Diagnosis of Malaria
title_full Evaluation of WarmStart Colorimetric Loop-Mediated Isothermal Amplification Assay for Diagnosis of Malaria
title_fullStr Evaluation of WarmStart Colorimetric Loop-Mediated Isothermal Amplification Assay for Diagnosis of Malaria
title_full_unstemmed Evaluation of WarmStart Colorimetric Loop-Mediated Isothermal Amplification Assay for Diagnosis of Malaria
title_sort evaluation of warmstart colorimetric loop-mediated isothermal amplification assay for diagnosis of malaria
publisher American Society of Tropical Medicine and Hygiene
publishDate 2020
url http://eprints.um.edu.my/36657/
_version_ 1787133826876571648
score 13.211869

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