DNA barcoding of simulium asakoae (Diptera: Simuliidae) from Northern Thailand

Previous studies suggested the presence of species complex in the so-called Simulium asakoaeTakaoka & Davies (Diptera: Simuliidae) in Thailand due to its high morphological variability and genetic divergence. To investigate whether the true S. asakoae is present inThailand, we performed a detail...

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Bibliographic Details
Main Authors: Low, Van Lun, Srisuka, Wichai, Saeung, Atiporn, Tan, Tiong Kai, Ya'cob, Zubaidah, Yeong, Yze Shiuan, Takaoka, Hiroyuki
Format: Article
Published: Oxford Univ Press Inc. 2020
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Online Access:http://eprints.um.edu.my/36448/
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Summary:Previous studies suggested the presence of species complex in the so-called Simulium asakoaeTakaoka & Davies (Diptera: Simuliidae) in Thailand due to its high morphological variability and genetic divergence. To investigate whether the true S. asakoae is present inThailand, we performed a detailed morphological identification of S. asakoae and compared its DNA barcodes with the morphospecies S. asakoae from Myanmar and the typical S. asakoae from Malaysia. Phylogenetic analysis revealed theThai materials analyzed in this study were indeed genetically similar with those from Myanmar and Malaysia, though genetic distances 0-2.27% were observed. We tentatively regard this divergence as intraspecific variation, and the automatic barcode gap discovery analysis further supports them as a single species.