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Eucalyptus concoction mediated synthesis of gold nanoparticles and its bioactive role xxplored via antimicrobial and cytotoxic studies

Biosynthesis of nanoparticles has now become a novel trend in addressing some of the environmental issues by adopting eco-friendly approaches in manoeuvring nanoparticles for various applications. Plants and micro-organisms have been the potential sources of the biological mode of synthesizing nanop...

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Main Authors:	Muthiah, Bavanilatha, Lovina, -, Arthi, -, Muthukrishnan, Lakshmipathy, Lett, J. Anita, Sagadevan, Suresh, Kesavan, Sudha, Vennila, Selvaraj, Khan, M. Ajmal, Hegazy, H. H., Ahmad, Naushad
Format:	Article
Published:	American Scientific Publishers 2020
Subjects:	T Technology (General) TS Manufactures
Online Access:	http://eprints.um.edu.my/36376/
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spelling	my.um.eprints.363762023-11-30T03:58:46Z http://eprints.um.edu.my/36376/ Eucalyptus concoction mediated synthesis of gold nanoparticles and its bioactive role xxplored via antimicrobial and cytotoxic studies Muthiah, Bavanilatha Lovina, - Arthi, - Muthukrishnan, Lakshmipathy Lett, J. Anita Sagadevan, Suresh Kesavan, Sudha Vennila, Selvaraj Khan, M. Ajmal Hegazy, H. H. Ahmad, Naushad T Technology (General) TS Manufactures Biosynthesis of nanoparticles has now become a novel trend in addressing some of the environmental issues by adopting eco-friendly approaches in manoeuvring nanoparticles for various applications. Plants and micro-organisms have been the potential sources of the biological mode of synthesizing nanoparticles as part of their bioremediation process. This principle has been harnessed for synthesizing nanoparticles either extra or intracellularly. In this line of phyto-mediated synthesis, eucalyptus buds have been used for synthesizing gold nanoparticles (Au NPs) under optimized laboratory conditions. The UV-visible spectrum of the Au NPs showed typical surface plasmon resonance at 550 nm (lambda(max)) with a crystalline phase measuring <100 nm in size and monodispersed as revealed from XRD, FESEM, and AFM analyses. The biological role of phytochemical concoction in reducing and stabilizing the Au NPs was clearly identified from FT-IR studies. The antimicrobial effect of the Au NPs against clinically important pathogens viz. Staphylococcus sp., Pseudomonas sp., Bacillus sp. and E. coli determined using the disk diffusion method showed no significant antibacterial effect at all concentrations. Cytotoxicity studies were carried using Vero and HEp-2 cell lines and the 50% inhibition concentration (IC50) was determined to be 1.25 mg and 0.625 mg/mL respectively. Au NPs with potential antimicrobial and anti-proliferative effects could found profound implications in the field of nanomedicine once the toxicity in vivo has been investigated. American Scientific Publishers 2020-10 Article PeerReviewed Muthiah, Bavanilatha and Lovina, - and Arthi, - and Muthukrishnan, Lakshmipathy and Lett, J. Anita and Sagadevan, Suresh and Kesavan, Sudha and Vennila, Selvaraj and Khan, M. Ajmal and Hegazy, H. H. and Ahmad, Naushad (2020) Eucalyptus concoction mediated synthesis of gold nanoparticles and its bioactive role xxplored via antimicrobial and cytotoxic studies. Journal of Nanoscience and Nanotechnology, 20 (10). pp. 6326-6333. ISSN 1533-4880, DOI https://doi.org/10.1166/jnn.2020.17897 <https://doi.org/10.1166/jnn.2020.17897>. 10.1166/jnn.2020.17897
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	T Technology (General) TS Manufactures
spellingShingle	T Technology (General) TS Manufactures Muthiah, Bavanilatha Lovina, - Arthi, - Muthukrishnan, Lakshmipathy Lett, J. Anita Sagadevan, Suresh Kesavan, Sudha Vennila, Selvaraj Khan, M. Ajmal Hegazy, H. H. Ahmad, Naushad Eucalyptus concoction mediated synthesis of gold nanoparticles and its bioactive role xxplored via antimicrobial and cytotoxic studies
description	Biosynthesis of nanoparticles has now become a novel trend in addressing some of the environmental issues by adopting eco-friendly approaches in manoeuvring nanoparticles for various applications. Plants and micro-organisms have been the potential sources of the biological mode of synthesizing nanoparticles as part of their bioremediation process. This principle has been harnessed for synthesizing nanoparticles either extra or intracellularly. In this line of phyto-mediated synthesis, eucalyptus buds have been used for synthesizing gold nanoparticles (Au NPs) under optimized laboratory conditions. The UV-visible spectrum of the Au NPs showed typical surface plasmon resonance at 550 nm (lambda(max)) with a crystalline phase measuring <100 nm in size and monodispersed as revealed from XRD, FESEM, and AFM analyses. The biological role of phytochemical concoction in reducing and stabilizing the Au NPs was clearly identified from FT-IR studies. The antimicrobial effect of the Au NPs against clinically important pathogens viz. Staphylococcus sp., Pseudomonas sp., Bacillus sp. and E. coli determined using the disk diffusion method showed no significant antibacterial effect at all concentrations. Cytotoxicity studies were carried using Vero and HEp-2 cell lines and the 50% inhibition concentration (IC50) was determined to be 1.25 mg and 0.625 mg/mL respectively. Au NPs with potential antimicrobial and anti-proliferative effects could found profound implications in the field of nanomedicine once the toxicity in vivo has been investigated.
format	Article
author	Muthiah, Bavanilatha Lovina, - Arthi, - Muthukrishnan, Lakshmipathy Lett, J. Anita Sagadevan, Suresh Kesavan, Sudha Vennila, Selvaraj Khan, M. Ajmal Hegazy, H. H. Ahmad, Naushad
author_facet	Muthiah, Bavanilatha Lovina, - Arthi, - Muthukrishnan, Lakshmipathy Lett, J. Anita Sagadevan, Suresh Kesavan, Sudha Vennila, Selvaraj Khan, M. Ajmal Hegazy, H. H. Ahmad, Naushad
author_sort	Muthiah, Bavanilatha
title	Eucalyptus concoction mediated synthesis of gold nanoparticles and its bioactive role xxplored via antimicrobial and cytotoxic studies
title_short	Eucalyptus concoction mediated synthesis of gold nanoparticles and its bioactive role xxplored via antimicrobial and cytotoxic studies
title_full	Eucalyptus concoction mediated synthesis of gold nanoparticles and its bioactive role xxplored via antimicrobial and cytotoxic studies
title_fullStr	Eucalyptus concoction mediated synthesis of gold nanoparticles and its bioactive role xxplored via antimicrobial and cytotoxic studies
title_full_unstemmed	Eucalyptus concoction mediated synthesis of gold nanoparticles and its bioactive role xxplored via antimicrobial and cytotoxic studies
title_sort	eucalyptus concoction mediated synthesis of gold nanoparticles and its bioactive role xxplored via antimicrobial and cytotoxic studies
publisher	American Scientific Publishers
publishDate	2020
url	http://eprints.um.edu.my/36376/
_version_	1784511815285211136
score	13.211869

Eucalyptus concoction mediated synthesis of gold nanoparticles and its bioactive role xxplored via antimicrobial and cytotoxic studies

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