Cover Image

Chlorhexidine hexametaphosphate as a coating for elastomeric ligatures with sustained antimicrobial properties: A laboratory study

Introduction: White spot lesions are a common side effect of orthodontic treatment. This laboratory study aimed to explore the suitability of chlorhexidine hexametaphosphate (CHX-HMP) as a coating for orthodontic elastomeric ligatures to provide sustained chlorhexidine (CHX) release. Methods: Dissol...

Full description

Saved in:
Bibliographic Details
Main Authors: Kamarudin, Yasmin, Skeats, Matthew K., Ireland, Anthony J., Barbour, Michele E.
Format: Article
Published: Elsevier 2020
Subjects:
R Medicine
RK Dentistry
Oral surger
Online Access:http://eprints.um.edu.my/36293/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.um.eprints.36293
record_format eprints
spelling my.um.eprints.362932023-11-29T12:50:26Z http://eprints.um.edu.my/36293/ Chlorhexidine hexametaphosphate as a coating for elastomeric ligatures with sustained antimicrobial properties: A laboratory study Kamarudin, Yasmin Skeats, Matthew K. Ireland, Anthony J. Barbour, Michele E. R Medicine RK Dentistry Oral surger Introduction: White spot lesions are a common side effect of orthodontic treatment. This laboratory study aimed to explore the suitability of chlorhexidine hexametaphosphate (CHX-HMP) as a coating for orthodontic elastomeric ligatures to provide sustained chlorhexidine (CHX) release. Methods: Dissolution kinetics of CHX-HMP were firstly explored using spectroscopy and a colorimetric phosphate assay. Elastomeric ligatures were categorized into 3 groups-acetone-conditioned, ethanol-conditioned, and as received-and were then immersed in 5 mM CHX-HMP suspension or 5 mM chlorhexidine digluconate solution and rinsed. CHX release was measured over 8 weeks, and the effects of conditioning and immersion on elastomeric force and extension at rupture and surface topography were investigated. Results: CHX-HMP exhibited a gradual equilibration that had not reached equilibrium within 8 weeks, releasing soluble CHX and a mixture of polyphosphate and orthophosphate. CHX digluconate-treated ligatures showed no CHX release, whereas CHX-HMP-treated ligatures showed varying degrees of release. As received, CHX-HMP-treated ligatures showed a modest release of CHX up to 7 days. Acetone conditioning did not enhance CHX-HMP uptake or subsequent CHX release and caused a deterioration in mechanical properties. Ethanol conditioning enhanced CHX-HMP uptake (63) and led to a sustained CHX release over 8 weeks without affecting mechanical properties. Conclusions: Within the inherent limitations of this in-vitro study, CHX-HMP led to a sustained release of CHX from orthodontic elastomeric ligatures after ethanol conditioning. Conditioned and coated elastomeric ligatures may ultimately find application in the prevention of white spot lesions in orthodontic patients. Elsevier 2020-11 Article PeerReviewed Kamarudin, Yasmin and Skeats, Matthew K. and Ireland, Anthony J. and Barbour, Michele E. (2020) Chlorhexidine hexametaphosphate as a coating for elastomeric ligatures with sustained antimicrobial properties: A laboratory study. American Journal of Orthodontics and Dentofacial Orthopedics, 158 (5). E73-E82. ISSN 0889-5406, DOI https://doi.org/10.1016/j.ajodo.2020.07.027 <https://doi.org/10.1016/j.ajodo.2020.07.027>. 10.1016/j.ajodo.2020.07.027
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
RK Dentistry
Oral surger
spellingShingle R Medicine
RK Dentistry
Oral surger
Kamarudin, Yasmin
Skeats, Matthew K.
Ireland, Anthony J.
Barbour, Michele E.
Chlorhexidine hexametaphosphate as a coating for elastomeric ligatures with sustained antimicrobial properties: A laboratory study
description Introduction: White spot lesions are a common side effect of orthodontic treatment. This laboratory study aimed to explore the suitability of chlorhexidine hexametaphosphate (CHX-HMP) as a coating for orthodontic elastomeric ligatures to provide sustained chlorhexidine (CHX) release. Methods: Dissolution kinetics of CHX-HMP were firstly explored using spectroscopy and a colorimetric phosphate assay. Elastomeric ligatures were categorized into 3 groups-acetone-conditioned, ethanol-conditioned, and as received-and were then immersed in 5 mM CHX-HMP suspension or 5 mM chlorhexidine digluconate solution and rinsed. CHX release was measured over 8 weeks, and the effects of conditioning and immersion on elastomeric force and extension at rupture and surface topography were investigated. Results: CHX-HMP exhibited a gradual equilibration that had not reached equilibrium within 8 weeks, releasing soluble CHX and a mixture of polyphosphate and orthophosphate. CHX digluconate-treated ligatures showed no CHX release, whereas CHX-HMP-treated ligatures showed varying degrees of release. As received, CHX-HMP-treated ligatures showed a modest release of CHX up to 7 days. Acetone conditioning did not enhance CHX-HMP uptake or subsequent CHX release and caused a deterioration in mechanical properties. Ethanol conditioning enhanced CHX-HMP uptake (63) and led to a sustained CHX release over 8 weeks without affecting mechanical properties. Conclusions: Within the inherent limitations of this in-vitro study, CHX-HMP led to a sustained release of CHX from orthodontic elastomeric ligatures after ethanol conditioning. Conditioned and coated elastomeric ligatures may ultimately find application in the prevention of white spot lesions in orthodontic patients.
format Article
author Kamarudin, Yasmin
Skeats, Matthew K.
Ireland, Anthony J.
Barbour, Michele E.
author_facet Kamarudin, Yasmin
Skeats, Matthew K.
Ireland, Anthony J.
Barbour, Michele E.
author_sort Kamarudin, Yasmin
title Chlorhexidine hexametaphosphate as a coating for elastomeric ligatures with sustained antimicrobial properties: A laboratory study
title_short Chlorhexidine hexametaphosphate as a coating for elastomeric ligatures with sustained antimicrobial properties: A laboratory study
title_full Chlorhexidine hexametaphosphate as a coating for elastomeric ligatures with sustained antimicrobial properties: A laboratory study
title_fullStr Chlorhexidine hexametaphosphate as a coating for elastomeric ligatures with sustained antimicrobial properties: A laboratory study
title_full_unstemmed Chlorhexidine hexametaphosphate as a coating for elastomeric ligatures with sustained antimicrobial properties: A laboratory study
title_sort chlorhexidine hexametaphosphate as a coating for elastomeric ligatures with sustained antimicrobial properties: a laboratory study
publisher Elsevier
publishDate 2020
url http://eprints.um.edu.my/36293/
_version_ 1783945853287792640
score 13.211869

Similar Items