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Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE

Different fatty acids have distinct effects on the survival of breast cancer cells, which could be mediated by fatty acid binding proteins (FABPs), a family of lipid chaperones. Due to the diverse structures of the members of FABP family, each FABP demonstrates distinct binding affinities to differe...

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Main Authors: Kwong, Soke Chee, Abd Jamil, Amira Hajirah, Rhodes, Anthony, Taib, Nur Aishah, Chung, Ivy
Format: Article
Published: Elsevier 2020
Subjects:
R Medicine
RS Pharmacy and materia medica
Online Access:http://eprints.um.edu.my/36221/
https://www.scopus.com/record/display.uri?eid=2-s2.0-85091080603&origin=resultslist&sort=plf-f&src=s&sid=d5c0fe81c8d4c799ccb17c3d64c91be5&sot=b&sdt=b&s=TITLE-ABS-KEY%28Fatty+acid+binding+protein+7+mediates+linoleic+acid-induced+cell+death+in+triple+negative+breast+cancer+cells+by+modulating+13-HODE%29&sl=146&sessionSearchId=d5c0fe81c8d4c799ccb17c3d64c91be5
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spelling my.um.eprints.362212023-11-29T04:37:35Z http://eprints.um.edu.my/36221/ Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE Kwong, Soke Chee Abd Jamil, Amira Hajirah Rhodes, Anthony Taib, Nur Aishah Chung, Ivy R Medicine RS Pharmacy and materia medica Different fatty acids have distinct effects on the survival of breast cancer cells, which could be mediated by fatty acid binding proteins (FABPs), a family of lipid chaperones. Due to the diverse structures of the members of FABP family, each FABP demonstrates distinct binding affinities to different fatty acids. Of note, FABP7 is predominantly expressed in triple negative breast cancer (TNBC), the most aggressive subtype of breast cancer. Yet, the role of FABP7 in modulating the effects of fatty acids on TNBC survival was unclear. In contrast to the high expression of FABP7 in human TNBC tumours, FABP7 protein was undetectable in TNBC cell lines. Hence, a FABP7 overexpression model was used for this study, in which the transduced TNBC cell lines (MDA-MB-231 and Hs578T) were treated with various monoand polyunsaturated fatty acids. Oleic acid (OA), docosahexaenoic acid (DHA) and arachidonic acid (AA) inhibited TNBC cell growth at high concentrations, with no differences resulted from FABP7 over expression. Interestingly, overexpression of FABP7 augmented linoleic acid-induced cell death in MDA-MB-231 cells. The increased cell death may be explained by a decrease in 13-HODE, a pro-tumorigenic oxidation product of linoleic acid. The phenotype was, however, attenuated with a rescue treatment using 25 nM 13-HODE. The decrease in 13-HODE was potentially due to fatty acid partitioning modulated by FABP7, as demonstrated by a 3-fold increase in fatty acid oxidation. Our findings suggest that linoleic acid could be a potential therapeutic strategy for FABP7-overexpressing TNBC patients. (C) 2020 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved. Elsevier 2020-12 Article PeerReviewed Kwong, Soke Chee and Abd Jamil, Amira Hajirah and Rhodes, Anthony and Taib, Nur Aishah and Chung, Ivy (2020) Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE. Biochimie, 179. pp. 23-31. ISSN 03009084, DOI https://doi.org/10.1016/j.biochi.2020.09.005 <https://doi.org/10.1016/j.biochi.2020.09.005>. https://www.scopus.com/record/display.uri?eid=2-s2.0-85091080603&origin=resultslist&sort=plf-f&src=s&sid=d5c0fe81c8d4c799ccb17c3d64c91be5&sot=b&sdt=b&s=TITLE-ABS-KEY%28Fatty+acid+binding+protein+7+mediates+linoleic+acid-induced+cell+death+in+triple+negative+breast+cancer+cells+by+modulating+13-HODE%29&sl=146&sessionSearchId=d5c0fe81c8d4c799ccb17c3d64c91be5 10.1016/j.biochi.2020.09.005
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
RS Pharmacy and materia medica
spellingShingle R Medicine
RS Pharmacy and materia medica
Kwong, Soke Chee
Abd Jamil, Amira Hajirah
Rhodes, Anthony
Taib, Nur Aishah
Chung, Ivy
Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE
description Different fatty acids have distinct effects on the survival of breast cancer cells, which could be mediated by fatty acid binding proteins (FABPs), a family of lipid chaperones. Due to the diverse structures of the members of FABP family, each FABP demonstrates distinct binding affinities to different fatty acids. Of note, FABP7 is predominantly expressed in triple negative breast cancer (TNBC), the most aggressive subtype of breast cancer. Yet, the role of FABP7 in modulating the effects of fatty acids on TNBC survival was unclear. In contrast to the high expression of FABP7 in human TNBC tumours, FABP7 protein was undetectable in TNBC cell lines. Hence, a FABP7 overexpression model was used for this study, in which the transduced TNBC cell lines (MDA-MB-231 and Hs578T) were treated with various monoand polyunsaturated fatty acids. Oleic acid (OA), docosahexaenoic acid (DHA) and arachidonic acid (AA) inhibited TNBC cell growth at high concentrations, with no differences resulted from FABP7 over expression. Interestingly, overexpression of FABP7 augmented linoleic acid-induced cell death in MDA-MB-231 cells. The increased cell death may be explained by a decrease in 13-HODE, a pro-tumorigenic oxidation product of linoleic acid. The phenotype was, however, attenuated with a rescue treatment using 25 nM 13-HODE. The decrease in 13-HODE was potentially due to fatty acid partitioning modulated by FABP7, as demonstrated by a 3-fold increase in fatty acid oxidation. Our findings suggest that linoleic acid could be a potential therapeutic strategy for FABP7-overexpressing TNBC patients. (C) 2020 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.
format Article
author Kwong, Soke Chee
Abd Jamil, Amira Hajirah
Rhodes, Anthony
Taib, Nur Aishah
Chung, Ivy
author_facet Kwong, Soke Chee
Abd Jamil, Amira Hajirah
Rhodes, Anthony
Taib, Nur Aishah
Chung, Ivy
author_sort Kwong, Soke Chee
title Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE
title_short Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE
title_full Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE
title_fullStr Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE
title_full_unstemmed Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE
title_sort fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-hode
publisher Elsevier
publishDate 2020
url http://eprints.um.edu.my/36221/
https://www.scopus.com/record/display.uri?eid=2-s2.0-85091080603&origin=resultslist&sort=plf-f&src=s&sid=d5c0fe81c8d4c799ccb17c3d64c91be5&sot=b&sdt=b&s=TITLE-ABS-KEY%28Fatty+acid+binding+protein+7+mediates+linoleic+acid-induced+cell+death+in+triple+negative+breast+cancer+cells+by+modulating+13-HODE%29&sl=146&sessionSearchId=d5c0fe81c8d4c799ccb17c3d64c91be5
_version_ 1783876654396866560
score 13.211869

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