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βeta-lactam resistance in upper respiratory tract pathogens isolated from a tertiary hospital in Malaysia

The rise of antimicrobial resistance (AMR) among clinically important bacteria, including respiratory pathogens, is a growing concern for public health worldwide. Common causative bacteria for upper respiratory tract infections (URTIs) include Streptococcus pneumoniae and Haemophilus influenzae, and...

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Main Authors:	Ngoi, Soo Tein, Muhamad, Anis Najwa, Teh, Cindy Shuan Ju, Chong, Chun Wie, Abdul Jabar, Kartini, Chai, Lay Ching, Leong, Kin Chong, Tee, Loong Hua, AbuBakar, Sazaly
Format:	Article
Published:	MDPI 2021
Subjects:	QR Microbiology
Online Access:	http://eprints.um.edu.my/36023/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-85121486068&doi=10.3390%2fpathogens10121602&partnerID=40&md5=4fccbc3f2acf3d987824a3a30bfb6f75
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spelling	my.um.eprints.360232022-10-31T07:22:10Z http://eprints.um.edu.my/36023/ βeta-lactam resistance in upper respiratory tract pathogens isolated from a tertiary hospital in Malaysia Ngoi, Soo Tein Muhamad, Anis Najwa Teh, Cindy Shuan Ju Chong, Chun Wie Abdul Jabar, Kartini Chai, Lay Ching Leong, Kin Chong Tee, Loong Hua AbuBakar, Sazaly QR Microbiology The rise of antimicrobial resistance (AMR) among clinically important bacteria, including respiratory pathogens, is a growing concern for public health worldwide. Common causative bacteria for upper respiratory tract infections (URTIs) include Streptococcus pneumoniae and Haemophilus influenzae, and sometimes Staphylococcus aureus. We assessed the β-lactam resistant trends and mechanisms of 150 URTI strains isolated in a tertiary care hospital in Kuala Lumpur Malaysia. High rates of non-susceptibility to penicillin G (38), amoxicillin-clavulanate (48), imipenem (60), and meropenem (56) were observed in S. pneumoniae. Frequent mutations at STMK and SRNVP motifs in PBP1a (41), SSNT motif in PBP2b (32), and STMK and LKSG motifs in PBP2x (41) were observed in S. pneumoniae. H. influenzae remained highly susceptible to most β-lactams, except for ampicillin. Approximately half of the ampicillin non-susceptible H. influenzae harboured PBP3 mutations (56) and only blaTEM was detected in the ampicillin-resistant strains (47). Methicillin-susceptible S. aureus (MSSA) strains were mostly resistant to penicillin G (92), with at least two-fold higher median minimum inhibitory concentrations (MIC) for all penicillin antibiotics (except ticarcillin) compared to S. pneumoniae and H. influenzae. Almost all URTI strains (88–100) were susceptible to cefcapene and flomoxef. Overall, β-lactam antibiotics except penicillins remained largely effective against URTI pathogens in this region. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. MDPI 2021-12 Article PeerReviewed Ngoi, Soo Tein and Muhamad, Anis Najwa and Teh, Cindy Shuan Ju and Chong, Chun Wie and Abdul Jabar, Kartini and Chai, Lay Ching and Leong, Kin Chong and Tee, Loong Hua and AbuBakar, Sazaly (2021) βeta-lactam resistance in upper respiratory tract pathogens isolated from a tertiary hospital in Malaysia. Pathogens, 10 (12). ISSN 2076-0817, DOI https://doi.org/10.3390/pathogens10121602 <https://doi.org/10.3390/pathogens10121602>. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85121486068&doi=10.3390%2fpathogens10121602&partnerID=40&md5=4fccbc3f2acf3d987824a3a30bfb6f75 10.3390/pathogens10121602
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	QR Microbiology
spellingShingle	QR Microbiology Ngoi, Soo Tein Muhamad, Anis Najwa Teh, Cindy Shuan Ju Chong, Chun Wie Abdul Jabar, Kartini Chai, Lay Ching Leong, Kin Chong Tee, Loong Hua AbuBakar, Sazaly βeta-lactam resistance in upper respiratory tract pathogens isolated from a tertiary hospital in Malaysia
description	The rise of antimicrobial resistance (AMR) among clinically important bacteria, including respiratory pathogens, is a growing concern for public health worldwide. Common causative bacteria for upper respiratory tract infections (URTIs) include Streptococcus pneumoniae and Haemophilus influenzae, and sometimes Staphylococcus aureus. We assessed the β-lactam resistant trends and mechanisms of 150 URTI strains isolated in a tertiary care hospital in Kuala Lumpur Malaysia. High rates of non-susceptibility to penicillin G (38), amoxicillin-clavulanate (48), imipenem (60), and meropenem (56) were observed in S. pneumoniae. Frequent mutations at STMK and SRNVP motifs in PBP1a (41), SSNT motif in PBP2b (32), and STMK and LKSG motifs in PBP2x (41) were observed in S. pneumoniae. H. influenzae remained highly susceptible to most β-lactams, except for ampicillin. Approximately half of the ampicillin non-susceptible H. influenzae harboured PBP3 mutations (56) and only blaTEM was detected in the ampicillin-resistant strains (47). Methicillin-susceptible S. aureus (MSSA) strains were mostly resistant to penicillin G (92), with at least two-fold higher median minimum inhibitory concentrations (MIC) for all penicillin antibiotics (except ticarcillin) compared to S. pneumoniae and H. influenzae. Almost all URTI strains (88–100) were susceptible to cefcapene and flomoxef. Overall, β-lactam antibiotics except penicillins remained largely effective against URTI pathogens in this region. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
format	Article
author	Ngoi, Soo Tein Muhamad, Anis Najwa Teh, Cindy Shuan Ju Chong, Chun Wie Abdul Jabar, Kartini Chai, Lay Ching Leong, Kin Chong Tee, Loong Hua AbuBakar, Sazaly
author_facet	Ngoi, Soo Tein Muhamad, Anis Najwa Teh, Cindy Shuan Ju Chong, Chun Wie Abdul Jabar, Kartini Chai, Lay Ching Leong, Kin Chong Tee, Loong Hua AbuBakar, Sazaly
author_sort	Ngoi, Soo Tein
title	βeta-lactam resistance in upper respiratory tract pathogens isolated from a tertiary hospital in Malaysia
title_short	βeta-lactam resistance in upper respiratory tract pathogens isolated from a tertiary hospital in Malaysia
title_full	βeta-lactam resistance in upper respiratory tract pathogens isolated from a tertiary hospital in Malaysia
title_fullStr	βeta-lactam resistance in upper respiratory tract pathogens isolated from a tertiary hospital in Malaysia
title_full_unstemmed	βeta-lactam resistance in upper respiratory tract pathogens isolated from a tertiary hospital in Malaysia
title_sort	βeta-lactam resistance in upper respiratory tract pathogens isolated from a tertiary hospital in malaysia
publisher	MDPI
publishDate	2021
url	http://eprints.um.edu.my/36023/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-85121486068&doi=10.3390%2fpathogens10121602&partnerID=40&md5=4fccbc3f2acf3d987824a3a30bfb6f75
_version_	1748703489940258816
score	13.211869

βeta-lactam resistance in upper respiratory tract pathogens isolated from a tertiary hospital in Malaysia

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