Rebaudioside A enhances LDL cholesterol uptake in HepG2 cells via suppression of HMGCR expression
Background: Rebaudioside A is one of the major diterpene glycosides found in Stevia had been reported to possess anti-hyperlipidemic effects. In this study, we explore the potential cholesterol-regulating mechanisms of Rebaudioside A in the human hepatoma (HepG2) cell line in comparison with simvast...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Published: |
2021
|
| Subjects: | |
| Online Access: | http://eprints.um.edu.my/35876/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
my.um.eprints.35876 |
|---|---|
| record_format |
eprints |
| spelling |
my.um.eprints.358762023-11-27T07:07:34Z http://eprints.um.edu.my/35876/ Rebaudioside A enhances LDL cholesterol uptake in HepG2 cells via suppression of HMGCR expression Ilias, A.N. Ismail, I.S. Hamzah, H. Mohd Mohidin, Taznim Begam Idris, M.F. Ajat, M. QD Chemistry QH301 Biology Background: Rebaudioside A is one of the major diterpene glycosides found in Stevia had been reported to possess anti-hyperlipidemic effects. In this study, we explore the potential cholesterol-regulating mechanisms of Rebaudioside A in the human hepatoma (HepG2) cell line in comparison with simvastatin. Methods: Cells were incubated with Rebaudioside A at several concentrations (0-10 μM) to determine the cytotoxicity by the MTT assay. Cells were treated with selected dosage (1 and 5 μM) in further experiments. Total cellular lipid was extracted by Bligh and Dyer method and subjected to quantitative colorimetric assay. To illustrate the effect of Rebaudioside A on cellular lipid droplets and low-density lipoprotein receptors, treated cells were subjected to immunofluorescence microscopy. Finally, we investigated the expression of experimental gene patterns of cells in response to treatment. Results: In this study, cytotoxicity of Rebaudioside A was determined at 27.72 μM. Treatment of cells with a higher concentration of Rebaudioside A promotes better hepatocellular cholesterol internalization and ameliorates cholesterol-regulating genes such as HMGCR, LDLR, and ACAT2. Conclusions: In conclusion, our data demonstrated that Rebaudioside A is capable to regulate cholesterol levels in HepG2 cells. Hence, we proposed that Rebaudioside A offers a potential alternative to statins for atherosclerosis therapy. © 2021. Reports of Biochemistry and Molecular Biology. All Rights Reserved. 2021-10 Article PeerReviewed Ilias, A.N. and Ismail, I.S. and Hamzah, H. and Mohd Mohidin, Taznim Begam and Idris, M.F. and Ajat, M. (2021) Rebaudioside A enhances LDL cholesterol uptake in HepG2 cells via suppression of HMGCR expression. Reports of Biochemistry and Molecular Biology, 10 (3). pp. 401-411. ISSN 2322-3480, |
| institution |
Universiti Malaya |
| building |
UM Library |
| collection |
Institutional Repository |
| continent |
Asia |
| country |
Malaysia |
| content_provider |
Universiti Malaya |
| content_source |
UM Research Repository |
| url_provider |
http://eprints.um.edu.my/ |
| topic |
QD Chemistry QH301 Biology |
| spellingShingle |
QD Chemistry QH301 Biology Ilias, A.N. Ismail, I.S. Hamzah, H. Mohd Mohidin, Taznim Begam Idris, M.F. Ajat, M. Rebaudioside A enhances LDL cholesterol uptake in HepG2 cells via suppression of HMGCR expression |
| description |
Background: Rebaudioside A is one of the major diterpene glycosides found in Stevia had been reported to possess anti-hyperlipidemic effects. In this study, we explore the potential cholesterol-regulating mechanisms of Rebaudioside A in the human hepatoma (HepG2) cell line in comparison with simvastatin. Methods: Cells were incubated with Rebaudioside A at several concentrations (0-10 μM) to determine the cytotoxicity by the MTT assay. Cells were treated with selected dosage (1 and 5 μM) in further experiments. Total cellular lipid was extracted by Bligh and Dyer method and subjected to quantitative colorimetric assay. To illustrate the effect of Rebaudioside A on cellular lipid droplets and low-density lipoprotein receptors, treated cells were subjected to immunofluorescence microscopy. Finally, we investigated the expression of experimental gene patterns of cells in response to treatment. Results: In this study, cytotoxicity of Rebaudioside A was determined at 27.72 μM. Treatment of cells with a higher concentration of Rebaudioside A promotes better hepatocellular cholesterol internalization and ameliorates cholesterol-regulating genes such as HMGCR, LDLR, and ACAT2. Conclusions: In conclusion, our data demonstrated that Rebaudioside A is capable to regulate cholesterol levels in HepG2 cells. Hence, we proposed that Rebaudioside A offers a potential alternative to statins for atherosclerosis therapy. © 2021. Reports of Biochemistry and Molecular Biology. All Rights Reserved. |
| format |
Article |
| author |
Ilias, A.N. Ismail, I.S. Hamzah, H. Mohd Mohidin, Taznim Begam Idris, M.F. Ajat, M. |
| author_facet |
Ilias, A.N. Ismail, I.S. Hamzah, H. Mohd Mohidin, Taznim Begam Idris, M.F. Ajat, M. |
| author_sort |
Ilias, A.N. |
| title |
Rebaudioside A enhances LDL cholesterol uptake in HepG2 cells via suppression of HMGCR expression |
| title_short |
Rebaudioside A enhances LDL cholesterol uptake in HepG2 cells via suppression of HMGCR expression |
| title_full |
Rebaudioside A enhances LDL cholesterol uptake in HepG2 cells via suppression of HMGCR expression |
| title_fullStr |
Rebaudioside A enhances LDL cholesterol uptake in HepG2 cells via suppression of HMGCR expression |
| title_full_unstemmed |
Rebaudioside A enhances LDL cholesterol uptake in HepG2 cells via suppression of HMGCR expression |
| title_sort |
rebaudioside a enhances ldl cholesterol uptake in hepg2 cells via suppression of hmgcr expression |
| publishDate |
2021 |
| url |
http://eprints.um.edu.my/35876/ |
| _version_ |
1783876643020865536 |
| score |
13.211869 |