Cover Image

Lactate dehydrogenase levels in the saliva of cigarette and e-cigarette smokers (vapers): A comparative analysis

Background: We examined the lactate dehydrogenase (LDH) enzyme levels in the saliva of vapers (e-cigarette users) and compared the data with cigarette smokers and a control group of non-smokers and non-vapers. Methods: Subjects were recruited among those responding to a social media announcement or...

Full description

Saved in:
Bibliographic Details
Main Authors: Pandarathodiyil, Anitha Krishnan, Ramanathan, Anand, Garg, Ranjana, Doss, Jennifer Geraldine, Abd Rahman, Fazliny, Wan Abdul Ghani, Wan Maria Nabillah, Warnakulasuriya, Saman
Format: Article
Published: Asian Pacific Organization for Cancer Prevention 2021
Subjects:
RK Dentistry
Online Access:http://eprints.um.edu.my/35788/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.um.eprints.35788
record_format eprints
spelling my.um.eprints.357882023-11-27T08:02:00Z http://eprints.um.edu.my/35788/ Lactate dehydrogenase levels in the saliva of cigarette and e-cigarette smokers (vapers): A comparative analysis Pandarathodiyil, Anitha Krishnan Ramanathan, Anand Garg, Ranjana Doss, Jennifer Geraldine Abd Rahman, Fazliny Wan Abdul Ghani, Wan Maria Nabillah Warnakulasuriya, Saman RK Dentistry Background: We examined the lactate dehydrogenase (LDH) enzyme levels in the saliva of vapers (e-cigarette users) and compared the data with cigarette smokers and a control group of non-smokers and non-vapers. Methods: Subjects were recruited among those responding to a social media announcement or patients attending the SEGi Oral Health Care Centre between May and December 2019, and among some staff at the centre. Five ml of unstimulated whole saliva was collected and salivary LDH enzyme activity levels were measured with a LDH colorimetric assay kit. Salivary LDH activity level was determined for each group and compared statistically. Results: Eighty-eight subjects were categorized into three groups (control n=30, smokers n=29, and vapers n=29). The mean ± standard deviation (SD) values for salivary LDH activity levels for vapers, smokers, and control groups were 35.15 ± 24.34 mU/ml, 30.82 ± 20.73 mU/ml, and 21.45 ± 15.30 mU/ml, respectively. The salivary LDH activity levels of smoker and vaper groups were significantly higher than in the control group (p = 0.031; 0.017). There was no significant difference of salivary LDH activity level in vapers when compared with smokers (p= 0.234). Conclusion: Our findings showed higher LDH levels in the saliva of vapers when compared with controls, confirming cytotoxic and harmful effects of e-cigarettes on the oral mucosa. © 2021. All Rights Reserved. Asian Pacific Organization for Cancer Prevention 2021 Article PeerReviewed Pandarathodiyil, Anitha Krishnan and Ramanathan, Anand and Garg, Ranjana and Doss, Jennifer Geraldine and Abd Rahman, Fazliny and Wan Abdul Ghani, Wan Maria Nabillah and Warnakulasuriya, Saman (2021) Lactate dehydrogenase levels in the saliva of cigarette and e-cigarette smokers (vapers): A comparative analysis. Asian Pacific Journal of Cancer Prevention, 22 (10). pp. 3227-3235. ISSN 15137368, DOI https://doi.org/10.31557/APJCP.2021.22.10.3227 <https://doi.org/10.31557/APJCP.2021.22.10.3227>. 10.31557/APJCP.2021.22.10.3227
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic RK Dentistry
spellingShingle RK Dentistry
Pandarathodiyil, Anitha Krishnan
Ramanathan, Anand
Garg, Ranjana
Doss, Jennifer Geraldine
Abd Rahman, Fazliny
Wan Abdul Ghani, Wan Maria Nabillah
Warnakulasuriya, Saman
Lactate dehydrogenase levels in the saliva of cigarette and e-cigarette smokers (vapers): A comparative analysis
description Background: We examined the lactate dehydrogenase (LDH) enzyme levels in the saliva of vapers (e-cigarette users) and compared the data with cigarette smokers and a control group of non-smokers and non-vapers. Methods: Subjects were recruited among those responding to a social media announcement or patients attending the SEGi Oral Health Care Centre between May and December 2019, and among some staff at the centre. Five ml of unstimulated whole saliva was collected and salivary LDH enzyme activity levels were measured with a LDH colorimetric assay kit. Salivary LDH activity level was determined for each group and compared statistically. Results: Eighty-eight subjects were categorized into three groups (control n=30, smokers n=29, and vapers n=29). The mean ± standard deviation (SD) values for salivary LDH activity levels for vapers, smokers, and control groups were 35.15 ± 24.34 mU/ml, 30.82 ± 20.73 mU/ml, and 21.45 ± 15.30 mU/ml, respectively. The salivary LDH activity levels of smoker and vaper groups were significantly higher than in the control group (p = 0.031; 0.017). There was no significant difference of salivary LDH activity level in vapers when compared with smokers (p= 0.234). Conclusion: Our findings showed higher LDH levels in the saliva of vapers when compared with controls, confirming cytotoxic and harmful effects of e-cigarettes on the oral mucosa. © 2021. All Rights Reserved.
format Article
author Pandarathodiyil, Anitha Krishnan
Ramanathan, Anand
Garg, Ranjana
Doss, Jennifer Geraldine
Abd Rahman, Fazliny
Wan Abdul Ghani, Wan Maria Nabillah
Warnakulasuriya, Saman
author_facet Pandarathodiyil, Anitha Krishnan
Ramanathan, Anand
Garg, Ranjana
Doss, Jennifer Geraldine
Abd Rahman, Fazliny
Wan Abdul Ghani, Wan Maria Nabillah
Warnakulasuriya, Saman
author_sort Pandarathodiyil, Anitha Krishnan
title Lactate dehydrogenase levels in the saliva of cigarette and e-cigarette smokers (vapers): A comparative analysis
title_short Lactate dehydrogenase levels in the saliva of cigarette and e-cigarette smokers (vapers): A comparative analysis
title_full Lactate dehydrogenase levels in the saliva of cigarette and e-cigarette smokers (vapers): A comparative analysis
title_fullStr Lactate dehydrogenase levels in the saliva of cigarette and e-cigarette smokers (vapers): A comparative analysis
title_full_unstemmed Lactate dehydrogenase levels in the saliva of cigarette and e-cigarette smokers (vapers): A comparative analysis
title_sort lactate dehydrogenase levels in the saliva of cigarette and e-cigarette smokers (vapers): a comparative analysis
publisher Asian Pacific Organization for Cancer Prevention
publishDate 2021
url http://eprints.um.edu.my/35788/
_version_ 1783876634819952640
score 13.244368

Similar Items