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In vitro propagation of Ananas comosus L. Merr. (Morris)

Ananas comosus L. Merr. (Morris) commonly known as pineapple, is an economically important fruit in Malaysia. Among the pineapple producers in the world, Malaysia has put massive efforts to fulfill the high domestic and global market demand. Several attempts have been made recently by integrating bi...

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Main Authors: Elias, H., Taha, R.M., Sani, H., Khamarul Huda, N.F., Ahmed, B.A., Abdullah, S., Zakeri, S.A.
Format: Article
Published: International Society for Horticultural Science 2021
Subjects:
Q Science (General)
Online Access:http://eprints.um.edu.my/35740/
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spelling my.um.eprints.357402023-11-09T03:40:55Z http://eprints.um.edu.my/35740/ In vitro propagation of Ananas comosus L. Merr. (Morris) Elias, H. Taha, R.M. Sani, H. Khamarul Huda, N.F. Ahmed, B.A. Abdullah, S. Zakeri, S.A. Elias, H. Q Science (General) Ananas comosus L. Merr. (Morris) commonly known as pineapple, is an economically important fruit in Malaysia. Among the pineapple producers in the world, Malaysia has put massive efforts to fulfill the high domestic and global market demand. Several attempts have been made recently by integrating biotechnology in conventional breeding concerning issues on the shortage production of pineapple fruits. The fruit productivity could be enhanced via a tissue culture system as it might provide the good quality of planting materials in industrial scale throughout the years. Hence, this study was designed to assess the effectiveness of utilizing tissue culture technique to produce sufficient plantlets of pineapple. The procedure involved culturing aseptic axillary buds excised from suckers in treatments with various concentrations of 6-benzylamino purine (BAP) to induce shoot multiplication. The suckers were successfully disinfected by soaking in 100 Clorox for 3 min with 80 survival and free contamination. The best result for this experiment was obtained in MS supplemented with 3.0 mg L-1 BAP, produced the highest no. of shoots per explant with the highest no. of leaves per explant. Besides, it was found that the treatments added with more than 3.0 mg L-1 BAP decreased the number of shoots and leaves per explant. Nevertheless, further advance study especially in quality control should be done to verify the quality of fruits meet the standard for commercialization. © 2021 International Society for Horticultural Science. All rights reserved. International Society for Horticultural Science 2021-09 Article PeerReviewed Elias, H. and Taha, R.M. and Sani, H. and Khamarul Huda, N.F. and Ahmed, B.A. and Abdullah, S. and Zakeri, S.A. and Elias, H. (2021) In vitro propagation of Ananas comosus L. Merr. (Morris). Acta Horticulturae, 1320. pp. 319-326. ISSN 05677572, DOI https://doi.org/10.17660/ActaHortic.2021.1320.42 <https://doi.org/10.17660/ActaHortic.2021.1320.42>. 10.17660/ActaHortic.2021.1320.42
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic Q Science (General)
spellingShingle Q Science (General)
Elias, H.
Taha, R.M.
Sani, H.
Khamarul Huda, N.F.
Ahmed, B.A.
Abdullah, S.
Zakeri, S.A.
Elias, H.
In vitro propagation of Ananas comosus L. Merr. (Morris)
description Ananas comosus L. Merr. (Morris) commonly known as pineapple, is an economically important fruit in Malaysia. Among the pineapple producers in the world, Malaysia has put massive efforts to fulfill the high domestic and global market demand. Several attempts have been made recently by integrating biotechnology in conventional breeding concerning issues on the shortage production of pineapple fruits. The fruit productivity could be enhanced via a tissue culture system as it might provide the good quality of planting materials in industrial scale throughout the years. Hence, this study was designed to assess the effectiveness of utilizing tissue culture technique to produce sufficient plantlets of pineapple. The procedure involved culturing aseptic axillary buds excised from suckers in treatments with various concentrations of 6-benzylamino purine (BAP) to induce shoot multiplication. The suckers were successfully disinfected by soaking in 100 Clorox for 3 min with 80 survival and free contamination. The best result for this experiment was obtained in MS supplemented with 3.0 mg L-1 BAP, produced the highest no. of shoots per explant with the highest no. of leaves per explant. Besides, it was found that the treatments added with more than 3.0 mg L-1 BAP decreased the number of shoots and leaves per explant. Nevertheless, further advance study especially in quality control should be done to verify the quality of fruits meet the standard for commercialization. © 2021 International Society for Horticultural Science. All rights reserved.
format Article
author Elias, H.
Taha, R.M.
Sani, H.
Khamarul Huda, N.F.
Ahmed, B.A.
Abdullah, S.
Zakeri, S.A.
Elias, H.
author_facet Elias, H.
Taha, R.M.
Sani, H.
Khamarul Huda, N.F.
Ahmed, B.A.
Abdullah, S.
Zakeri, S.A.
Elias, H.
author_sort Elias, H.
title In vitro propagation of Ananas comosus L. Merr. (Morris)
title_short In vitro propagation of Ananas comosus L. Merr. (Morris)
title_full In vitro propagation of Ananas comosus L. Merr. (Morris)
title_fullStr In vitro propagation of Ananas comosus L. Merr. (Morris)
title_full_unstemmed In vitro propagation of Ananas comosus L. Merr. (Morris)
title_sort in vitro propagation of ananas comosus l. merr. (morris)
publisher International Society for Horticultural Science
publishDate 2021
url http://eprints.um.edu.my/35740/
_version_ 1783876631177199616
score 13.211869

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