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Enhanced killing of multidrug-resistant Pseudomonas aeruginosa ATCC10145 through a combined action of antibiotics and bacteriocin from Pediococcus pentosaceus TU2

Due to its rapid development of resistance against most conventional antibiotics, there is an urgent need to develop new antimicrobial agents and strategies to overcome the challenges in combating multidrug-resistant Pseudomonas aeruginosa infections. This study aimed to determine the antipseudomona...

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Main Authors: Suffian, Suffi Nurul Husna, Tan, Boon Chin, Lim, Yin Sze
Format: Article
Published: Malaysian Soc Microbiology 2021
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QR Microbiology
Online Access:http://eprints.um.edu.my/35688/
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85122272253&doi=10.21161%2fmjm.211246&partnerID=40&md5=02d17ef033d4e9710c485dcc71be277e
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spelling my.um.eprints.356882022-10-19T03:30:53Z http://eprints.um.edu.my/35688/ Enhanced killing of multidrug-resistant Pseudomonas aeruginosa ATCC10145 through a combined action of antibiotics and bacteriocin from Pediococcus pentosaceus TU2 Suffian, Suffi Nurul Husna Tan, Boon Chin Lim, Yin Sze QR Microbiology Due to its rapid development of resistance against most conventional antibiotics, there is an urgent need to develop new antimicrobial agents and strategies to overcome the challenges in combating multidrug-resistant Pseudomonas aeruginosa infections. This study aimed to determine the antipseudomonal potency of bacteriocin produced by Pediococcus pentosaceus TU2 when combined with conventional antibiotics. Methodology and results: The checkerboard method and time-kill assay were conducted to investigate the antagonism interaction and kinetics of the bacteriocin TU2 and selected antibiotics against Pseudomonas aeruginosa ATCC10145. The scanning electron microscope (SEM) was used to observe the cell surface morphological changes of the treated P. aeruginosa ATCC10145. The combination of bacteriocin TU2 with ciprofloxacin and tetracycline resulted in a 4-fold reduction in minimum inhibitory concentration (MIC) and a fractional inhibitory concentration index (ΣFICI) of 0.5, indicating a synergistic interaction against P. aeruginosa ATCC10145. Similarly, the time-kill assay showed that the combination of bacteriocins TU2 respectively with chloramphenicol and tetracycline exerted enhanced bactericidal effect at 8 h and 10 h of treatments compared to treatment with antimicrobial agents alone. Results from SEM suggested that bacteriocin TU2 might cause pore formation on cells and thus enhanced the membrane permeability of antibiotics and intensified the membrane leakage that led to cell death of P. aeruginosa ATCC10145. Conclusion, significance and impact of study: The combined antagonistic effect of bacteriocin TU2 and antibiotics could be a promising strategy in combating P. aeruginosa infections and may be applied in therapeutic industries. © 2021. Malaysian Journal of Microbiology. All Rights Reserved. Malaysian Soc Microbiology 2021-12 Article PeerReviewed Suffian, Suffi Nurul Husna and Tan, Boon Chin and Lim, Yin Sze (2021) Enhanced killing of multidrug-resistant Pseudomonas aeruginosa ATCC10145 through a combined action of antibiotics and bacteriocin from Pediococcus pentosaceus TU2. Malaysian Journal of Microbiology, 17 (6). pp. 668-680. ISSN 1823-8262, DOI https://doi.org/10.21161/mjm.211246 <https://doi.org/10.21161/mjm.211246>. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85122272253&doi=10.21161%2fmjm.211246&partnerID=40&md5=02d17ef033d4e9710c485dcc71be277e 10.21161/mjm.211246
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QR Microbiology
spellingShingle QR Microbiology
Suffian, Suffi Nurul Husna
Tan, Boon Chin
Lim, Yin Sze
Enhanced killing of multidrug-resistant Pseudomonas aeruginosa ATCC10145 through a combined action of antibiotics and bacteriocin from Pediococcus pentosaceus TU2
description Due to its rapid development of resistance against most conventional antibiotics, there is an urgent need to develop new antimicrobial agents and strategies to overcome the challenges in combating multidrug-resistant Pseudomonas aeruginosa infections. This study aimed to determine the antipseudomonal potency of bacteriocin produced by Pediococcus pentosaceus TU2 when combined with conventional antibiotics. Methodology and results: The checkerboard method and time-kill assay were conducted to investigate the antagonism interaction and kinetics of the bacteriocin TU2 and selected antibiotics against Pseudomonas aeruginosa ATCC10145. The scanning electron microscope (SEM) was used to observe the cell surface morphological changes of the treated P. aeruginosa ATCC10145. The combination of bacteriocin TU2 with ciprofloxacin and tetracycline resulted in a 4-fold reduction in minimum inhibitory concentration (MIC) and a fractional inhibitory concentration index (ΣFICI) of 0.5, indicating a synergistic interaction against P. aeruginosa ATCC10145. Similarly, the time-kill assay showed that the combination of bacteriocins TU2 respectively with chloramphenicol and tetracycline exerted enhanced bactericidal effect at 8 h and 10 h of treatments compared to treatment with antimicrobial agents alone. Results from SEM suggested that bacteriocin TU2 might cause pore formation on cells and thus enhanced the membrane permeability of antibiotics and intensified the membrane leakage that led to cell death of P. aeruginosa ATCC10145. Conclusion, significance and impact of study: The combined antagonistic effect of bacteriocin TU2 and antibiotics could be a promising strategy in combating P. aeruginosa infections and may be applied in therapeutic industries. © 2021. Malaysian Journal of Microbiology. All Rights Reserved.
format Article
author Suffian, Suffi Nurul Husna
Tan, Boon Chin
Lim, Yin Sze
author_facet Suffian, Suffi Nurul Husna
Tan, Boon Chin
Lim, Yin Sze
author_sort Suffian, Suffi Nurul Husna
title Enhanced killing of multidrug-resistant Pseudomonas aeruginosa ATCC10145 through a combined action of antibiotics and bacteriocin from Pediococcus pentosaceus TU2
title_short Enhanced killing of multidrug-resistant Pseudomonas aeruginosa ATCC10145 through a combined action of antibiotics and bacteriocin from Pediococcus pentosaceus TU2
title_full Enhanced killing of multidrug-resistant Pseudomonas aeruginosa ATCC10145 through a combined action of antibiotics and bacteriocin from Pediococcus pentosaceus TU2
title_fullStr Enhanced killing of multidrug-resistant Pseudomonas aeruginosa ATCC10145 through a combined action of antibiotics and bacteriocin from Pediococcus pentosaceus TU2
title_full_unstemmed Enhanced killing of multidrug-resistant Pseudomonas aeruginosa ATCC10145 through a combined action of antibiotics and bacteriocin from Pediococcus pentosaceus TU2
title_sort enhanced killing of multidrug-resistant pseudomonas aeruginosa atcc10145 through a combined action of antibiotics and bacteriocin from pediococcus pentosaceus tu2
publisher Malaysian Soc Microbiology
publishDate 2021
url http://eprints.um.edu.my/35688/
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85122272253&doi=10.21161%2fmjm.211246&partnerID=40&md5=02d17ef033d4e9710c485dcc71be277e
_version_ 1748181090971942912
score 13.211869

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