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Alkaline phosphatase activity assessment Of two endodontic materials: a preliminary study

Objective: An in vitro assessment of MG-63 human osteosarcoma cells' alkaline phosphatase (ALP) activity when in contact with calcium hydroxide powder (CH), paste (CHP) and grey mineral trioxide aggregate (MTA). Methods: MG-63 cells were seeded to the three selected materials for durations of 0...

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Main Authors: Rajan, S., Awang, H., Devi, S., PooP, A.H., Hassan, H.
Format: Article
Language:English
Published: 2008
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/3553/1/Alkaline_phosphatase_activity_assessment_of_two_endodontic_materials-_a_preliminary_study.pdf
http://eprints.um.edu.my/3553/
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spelling my.um.eprints.35532012-08-06T01:35:15Z http://eprints.um.edu.my/3553/ Alkaline phosphatase activity assessment Of two endodontic materials: a preliminary study Rajan, S. Awang, H. Devi, S. PooP, A.H. Hassan, H. R Medicine Objective: An in vitro assessment of MG-63 human osteosarcoma cells' alkaline phosphatase (ALP) activity when in contact with calcium hydroxide powder (CH), paste (CHP) and grey mineral trioxide aggregate (MTA). Methods: MG-63 cells were seeded to the three selected materials for durations of 0.25, 0.5, 1, 24, 48 and 72 hours. BCIP-NBT assay was used and ALP activity quantified using ELISA reader at 410 nm. Results: The overall analysis for ALP activity indicated significant interaction between test materials and control (maintenance medium). Subsequently, the test materials were paired and analysed for initial (0.25, 0.5, 1 hour) and delayed response (24, 48 and 72 hours). During the initial response, CH exhibited an increased ALP activity compared to MTA. This interaction was not dependant on duration. The delayed response exhibited elevated ALP activity with CHP when compared to MTA and CH. The interaction of CHP was dependant on duration. Conclusion: All three materials exhibited increased ALP activity. 2008 Article PeerReviewed application/pdf en http://eprints.um.edu.my/3553/1/Alkaline_phosphatase_activity_assessment_of_two_endodontic_materials-_a_preliminary_study.pdf Rajan, S. and Awang, H. and Devi, S. and PooP, A.H. and Hassan, H. (2008) Alkaline phosphatase activity assessment Of two endodontic materials: a preliminary study. Annals of Dentistry, 15 (1). pp. 5-10. ISSN 0128-7532
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic R Medicine
spellingShingle R Medicine
Rajan, S.
Awang, H.
Devi, S.
PooP, A.H.
Hassan, H.
Alkaline phosphatase activity assessment Of two endodontic materials: a preliminary study
description Objective: An in vitro assessment of MG-63 human osteosarcoma cells' alkaline phosphatase (ALP) activity when in contact with calcium hydroxide powder (CH), paste (CHP) and grey mineral trioxide aggregate (MTA). Methods: MG-63 cells were seeded to the three selected materials for durations of 0.25, 0.5, 1, 24, 48 and 72 hours. BCIP-NBT assay was used and ALP activity quantified using ELISA reader at 410 nm. Results: The overall analysis for ALP activity indicated significant interaction between test materials and control (maintenance medium). Subsequently, the test materials were paired and analysed for initial (0.25, 0.5, 1 hour) and delayed response (24, 48 and 72 hours). During the initial response, CH exhibited an increased ALP activity compared to MTA. This interaction was not dependant on duration. The delayed response exhibited elevated ALP activity with CHP when compared to MTA and CH. The interaction of CHP was dependant on duration. Conclusion: All three materials exhibited increased ALP activity.
format Article
author Rajan, S.
Awang, H.
Devi, S.
PooP, A.H.
Hassan, H.
author_facet Rajan, S.
Awang, H.
Devi, S.
PooP, A.H.
Hassan, H.
author_sort Rajan, S.
title Alkaline phosphatase activity assessment Of two endodontic materials: a preliminary study
title_short Alkaline phosphatase activity assessment Of two endodontic materials: a preliminary study
title_full Alkaline phosphatase activity assessment Of two endodontic materials: a preliminary study
title_fullStr Alkaline phosphatase activity assessment Of two endodontic materials: a preliminary study
title_full_unstemmed Alkaline phosphatase activity assessment Of two endodontic materials: a preliminary study
title_sort alkaline phosphatase activity assessment of two endodontic materials: a preliminary study
publishDate 2008
url http://eprints.um.edu.my/3553/1/Alkaline_phosphatase_activity_assessment_of_two_endodontic_materials-_a_preliminary_study.pdf
http://eprints.um.edu.my/3553/
_version_ 1643687146518740992
score 13.211869

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