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Transcriptomic evidence for the up-regulation of pentose phosphate pathway during the morphogenesis of Ganoderma lucidum

Ganoderma lucidum is a medicinal mushroom with a broad spectrum of secondary metabolites with antibacterial and antiviral properties that can be leveraged as lead compounds for drug development. The fungus is known to have two distinct forms corresponding to different culture conditions that result...

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Main Authors: Cheng, Poh Guat, Teoh, Teow Chong, Rizman-Idid, Mohammed, Abdulrahman, Ammar Yasir
Format: Article
Published: Faculty of Science, Chiang Mai University 2021
Subjects:
GE Environmental Sciences
QK Botany
Online Access:http://eprints.um.edu.my/35317/
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spelling my.um.eprints.353172022-10-14T05:53:18Z http://eprints.um.edu.my/35317/ Transcriptomic evidence for the up-regulation of pentose phosphate pathway during the morphogenesis of Ganoderma lucidum Cheng, Poh Guat Teoh, Teow Chong Rizman-Idid, Mohammed Abdulrahman, Ammar Yasir GE Environmental Sciences QK Botany Ganoderma lucidum is a medicinal mushroom with a broad spectrum of secondary metabolites with antibacterial and antiviral properties that can be leveraged as lead compounds for drug development. The fungus is known to have two distinct forms corresponding to different culture conditions that result in kidney and antler shapes. Upon culturing in a dark and enclosed environment with elevated CO2 concentration, the fungus adopts an antler-shaped fruiting body instead of the typical kidney-shaped morphology. The mechanism of G. lucidum phenotypic alteration yet to be understood. By using the RNA-Seq transcriptome de novo assembly approach, the differential gene expression (DGE) profiles during the morphogenesis of the two fungal shapes revealed that the fungal hydrophobin gene SC3 was up-regulated for the production of antler-shaped fruiting body. The analysis also indicated up-regulation of the pentose phosphate pathway (PPP) as the key metabolic pathway for converting glucose into various types of sugars for the survival of the fungus. Numerous unigenes for the biosynthesis of triterpenoids, which include the key enzymes of lanosterol synthase (LS), cytochromes P450 (P450) and uridine diphosphate glycosyltransferases (UGTs) genes were also identified and significantly up-regulated from the DGE analysis associated with the morphogenesis of antler-shaped G. lucidum. Faculty of Science, Chiang Mai University 2021-11 Article PeerReviewed Cheng, Poh Guat and Teoh, Teow Chong and Rizman-Idid, Mohammed and Abdulrahman, Ammar Yasir (2021) Transcriptomic evidence for the up-regulation of pentose phosphate pathway during the morphogenesis of Ganoderma lucidum. Chiang Mai Journal of Science, 48 (6). pp. 1444-1462. ISSN 0125-2526,
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic GE Environmental Sciences
QK Botany
spellingShingle GE Environmental Sciences
QK Botany
Cheng, Poh Guat
Teoh, Teow Chong
Rizman-Idid, Mohammed
Abdulrahman, Ammar Yasir
Transcriptomic evidence for the up-regulation of pentose phosphate pathway during the morphogenesis of Ganoderma lucidum
description Ganoderma lucidum is a medicinal mushroom with a broad spectrum of secondary metabolites with antibacterial and antiviral properties that can be leveraged as lead compounds for drug development. The fungus is known to have two distinct forms corresponding to different culture conditions that result in kidney and antler shapes. Upon culturing in a dark and enclosed environment with elevated CO2 concentration, the fungus adopts an antler-shaped fruiting body instead of the typical kidney-shaped morphology. The mechanism of G. lucidum phenotypic alteration yet to be understood. By using the RNA-Seq transcriptome de novo assembly approach, the differential gene expression (DGE) profiles during the morphogenesis of the two fungal shapes revealed that the fungal hydrophobin gene SC3 was up-regulated for the production of antler-shaped fruiting body. The analysis also indicated up-regulation of the pentose phosphate pathway (PPP) as the key metabolic pathway for converting glucose into various types of sugars for the survival of the fungus. Numerous unigenes for the biosynthesis of triterpenoids, which include the key enzymes of lanosterol synthase (LS), cytochromes P450 (P450) and uridine diphosphate glycosyltransferases (UGTs) genes were also identified and significantly up-regulated from the DGE analysis associated with the morphogenesis of antler-shaped G. lucidum.
format Article
author Cheng, Poh Guat
Teoh, Teow Chong
Rizman-Idid, Mohammed
Abdulrahman, Ammar Yasir
author_facet Cheng, Poh Guat
Teoh, Teow Chong
Rizman-Idid, Mohammed
Abdulrahman, Ammar Yasir
author_sort Cheng, Poh Guat
title Transcriptomic evidence for the up-regulation of pentose phosphate pathway during the morphogenesis of Ganoderma lucidum
title_short Transcriptomic evidence for the up-regulation of pentose phosphate pathway during the morphogenesis of Ganoderma lucidum
title_full Transcriptomic evidence for the up-regulation of pentose phosphate pathway during the morphogenesis of Ganoderma lucidum
title_fullStr Transcriptomic evidence for the up-regulation of pentose phosphate pathway during the morphogenesis of Ganoderma lucidum
title_full_unstemmed Transcriptomic evidence for the up-regulation of pentose phosphate pathway during the morphogenesis of Ganoderma lucidum
title_sort transcriptomic evidence for the up-regulation of pentose phosphate pathway during the morphogenesis of ganoderma lucidum
publisher Faculty of Science, Chiang Mai University
publishDate 2021
url http://eprints.um.edu.my/35317/
_version_ 1748181075336626176
score 13.211869

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