Cover Image

Isolation, identification, cultivation and determination of antimicrobial beta-glucan from a wild-termite mushroom Termitomyces heimii RFES 230662

During the monsoon season, a wild-termite mushroom Termitomyces heimii RFES 230662 (THR2) was successfully isolated from Negeri Sembilan, Malaysia. The mushroom was morphologically labelled based on its stipe, pileus, and the budding mycelia from the termites nest. Genetic component of THR2 (300 bp)...

Full description

Saved in:
Bibliographic Details
Main Authors: Ahmad, Rahayu, Sellathoroe, Srivani, Ngadi, Ehwan, Saharuddin, Tengku Shafazila Tengku, Zakaria, Iffah Izzati, Selvakumaran, Suguna, Wan-Mohtar, Wan Abd Al Qadr Imad
Format: Article
Published: Elsevier 2021
Subjects:
QK Botany
R Medicine
RV Botanic, Thomsonian, and eclectic medicine
SB Plant culture
Online Access:http://eprints.um.edu.my/35003/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.um.eprints.35003
record_format eprints
spelling my.um.eprints.350032022-09-08T03:09:23Z http://eprints.um.edu.my/35003/ Isolation, identification, cultivation and determination of antimicrobial beta-glucan from a wild-termite mushroom Termitomyces heimii RFES 230662 Ahmad, Rahayu Sellathoroe, Srivani Ngadi, Ehwan Saharuddin, Tengku Shafazila Tengku Zakaria, Iffah Izzati Selvakumaran, Suguna Wan-Mohtar, Wan Abd Al Qadr Imad QK Botany R Medicine RV Botanic, Thomsonian, and eclectic medicine SB Plant culture During the monsoon season, a wild-termite mushroom Termitomyces heimii RFES 230662 (THR2) was successfully isolated from Negeri Sembilan, Malaysia. The mushroom was morphologically labelled based on its stipe, pileus, and the budding mycelia from the termites nest. Genetic component of THR2 (300 bp) was sequenced and found to be 99% identical to Termitomyces sp. strain. The evolutionary distance (K-nuc) of the isolate justified THR2 as T. heimii species. Two THR2 extracts were prepared from fruiting body (FB) and mycelial biomass (MB) for antimicrobial responses. THR2 was cultivated in a submerged-fermentation (SF) producing 8.55 g/L of MB, 0.80 g/L of endopolysaccharide (ENS) and 1.44 g/L of exopolysaccharide (EPS), respectively. For THR2-FB, two ENS extracts were obtained using hot (3.20 g/L) and cold-water (1.36 g/L) treatments. FTIR spectra analysis verified all polysaccharide as beta-glucan when compared to laminarin standard. Those beta-glucan possess antibacterial properties with highest response shown by beta-glucan-FB compared to beta-glucan-MB extracts. These findings serve as the blueprint for the production of beta-glucan from a rare termite mushroom. Elsevier 2021-10 Article PeerReviewed Ahmad, Rahayu and Sellathoroe, Srivani and Ngadi, Ehwan and Saharuddin, Tengku Shafazila Tengku and Zakaria, Iffah Izzati and Selvakumaran, Suguna and Wan-Mohtar, Wan Abd Al Qadr Imad (2021) Isolation, identification, cultivation and determination of antimicrobial beta-glucan from a wild-termite mushroom Termitomyces heimii RFES 230662. Biocatalysis and Agricultural Biotechnology, 37. ISSN 1878-8181, DOI https://doi.org/10.1016/j.bcab.2021.102187 <https://doi.org/10.1016/j.bcab.2021.102187>. 10.1016/j.bcab.2021.102187
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QK Botany
R Medicine
RV Botanic, Thomsonian, and eclectic medicine
SB Plant culture
spellingShingle QK Botany
R Medicine
RV Botanic, Thomsonian, and eclectic medicine
SB Plant culture
Ahmad, Rahayu
Sellathoroe, Srivani
Ngadi, Ehwan
Saharuddin, Tengku Shafazila Tengku
Zakaria, Iffah Izzati
Selvakumaran, Suguna
Wan-Mohtar, Wan Abd Al Qadr Imad
Isolation, identification, cultivation and determination of antimicrobial beta-glucan from a wild-termite mushroom Termitomyces heimii RFES 230662
description During the monsoon season, a wild-termite mushroom Termitomyces heimii RFES 230662 (THR2) was successfully isolated from Negeri Sembilan, Malaysia. The mushroom was morphologically labelled based on its stipe, pileus, and the budding mycelia from the termites nest. Genetic component of THR2 (300 bp) was sequenced and found to be 99% identical to Termitomyces sp. strain. The evolutionary distance (K-nuc) of the isolate justified THR2 as T. heimii species. Two THR2 extracts were prepared from fruiting body (FB) and mycelial biomass (MB) for antimicrobial responses. THR2 was cultivated in a submerged-fermentation (SF) producing 8.55 g/L of MB, 0.80 g/L of endopolysaccharide (ENS) and 1.44 g/L of exopolysaccharide (EPS), respectively. For THR2-FB, two ENS extracts were obtained using hot (3.20 g/L) and cold-water (1.36 g/L) treatments. FTIR spectra analysis verified all polysaccharide as beta-glucan when compared to laminarin standard. Those beta-glucan possess antibacterial properties with highest response shown by beta-glucan-FB compared to beta-glucan-MB extracts. These findings serve as the blueprint for the production of beta-glucan from a rare termite mushroom.
format Article
author Ahmad, Rahayu
Sellathoroe, Srivani
Ngadi, Ehwan
Saharuddin, Tengku Shafazila Tengku
Zakaria, Iffah Izzati
Selvakumaran, Suguna
Wan-Mohtar, Wan Abd Al Qadr Imad
author_facet Ahmad, Rahayu
Sellathoroe, Srivani
Ngadi, Ehwan
Saharuddin, Tengku Shafazila Tengku
Zakaria, Iffah Izzati
Selvakumaran, Suguna
Wan-Mohtar, Wan Abd Al Qadr Imad
author_sort Ahmad, Rahayu
title Isolation, identification, cultivation and determination of antimicrobial beta-glucan from a wild-termite mushroom Termitomyces heimii RFES 230662
title_short Isolation, identification, cultivation and determination of antimicrobial beta-glucan from a wild-termite mushroom Termitomyces heimii RFES 230662
title_full Isolation, identification, cultivation and determination of antimicrobial beta-glucan from a wild-termite mushroom Termitomyces heimii RFES 230662
title_fullStr Isolation, identification, cultivation and determination of antimicrobial beta-glucan from a wild-termite mushroom Termitomyces heimii RFES 230662
title_full_unstemmed Isolation, identification, cultivation and determination of antimicrobial beta-glucan from a wild-termite mushroom Termitomyces heimii RFES 230662
title_sort isolation, identification, cultivation and determination of antimicrobial beta-glucan from a wild-termite mushroom termitomyces heimii rfes 230662
publisher Elsevier
publishDate 2021
url http://eprints.um.edu.my/35003/
_version_ 1744649200260349952
score 13.211869

Similar Items