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Biosafety evaluation and detection of shrimp viruses on field samples using dual priming oligonucleotide (DPO) system based multiplex PCR assay

Disease is the current major challenge and issue in shrimp aquaculture as it can be spread easily causing global outbreak. The risk of disease outbreak in shrimp populations can be further minimized with rapid and accurate diagnostic method. This study aims to develop a dual priming oligonucleotide...

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Main Authors: See, SiouNing Aileen, Goh, Zee Hong, Chan, Yen Yew, Chong, Khai En, Tan, Geok Yuan Annie, Bhassu, Subha, Othman, Rofina Yasmin
Format: Article
Published: Elsevier 2021
Subjects:
SH Aquaculture. Fisheries. Angling
Online Access:http://eprints.um.edu.my/34829/
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spelling my.um.eprints.348292022-05-28T04:58:30Z http://eprints.um.edu.my/34829/ Biosafety evaluation and detection of shrimp viruses on field samples using dual priming oligonucleotide (DPO) system based multiplex PCR assay See, SiouNing Aileen Goh, Zee Hong Chan, Yen Yew Chong, Khai En Tan, Geok Yuan Annie Bhassu, Subha Othman, Rofina Yasmin SH Aquaculture. Fisheries. Angling Disease is the current major challenge and issue in shrimp aquaculture as it can be spread easily causing global outbreak. The risk of disease outbreak in shrimp populations can be further minimized with rapid and accurate diagnostic method. This study aims to develop a dual priming oligonucleotide (DPO) based multiplex PCR system for simultaneous detection of shrimp DNA and RNA viruses. This system targets Baculovirus Penaei (BP), Monodon Baculovirus (MB), Infectious Hypodermal, Haematopoietic Necrosis Virus (IHHNV) and White Spot Syndrome Virus (WSSV), Macrobrachium rosenbergii nodavirus (MrNV), Infectious Myonecrosis Virus (IMNV), Taura Syndrome Virus (TSV) and Yellow Head Virus (YHV). The shrimp samples collected from shrimp farm were diagnosed to be infected with YHV infection in the 2nd cycle in year 2018 with 80% screening rate (4/5). DPO based multiplex PCR system may facilitate the rapid and effective screening of shrimp viruses in laboratory. Elsevier 2021-06 Article PeerReviewed See, SiouNing Aileen and Goh, Zee Hong and Chan, Yen Yew and Chong, Khai En and Tan, Geok Yuan Annie and Bhassu, Subha and Othman, Rofina Yasmin (2021) Biosafety evaluation and detection of shrimp viruses on field samples using dual priming oligonucleotide (DPO) system based multiplex PCR assay. Gene Reports, 23. ISSN 2452-0144, DOI https://doi.org/10.1016/j.genrep.2021.101158 <https://doi.org/10.1016/j.genrep.2021.101158>. 10.1016/j.genrep.2021.101158
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic SH Aquaculture. Fisheries. Angling
spellingShingle SH Aquaculture. Fisheries. Angling
See, SiouNing Aileen
Goh, Zee Hong
Chan, Yen Yew
Chong, Khai En
Tan, Geok Yuan Annie
Bhassu, Subha
Othman, Rofina Yasmin
Biosafety evaluation and detection of shrimp viruses on field samples using dual priming oligonucleotide (DPO) system based multiplex PCR assay
description Disease is the current major challenge and issue in shrimp aquaculture as it can be spread easily causing global outbreak. The risk of disease outbreak in shrimp populations can be further minimized with rapid and accurate diagnostic method. This study aims to develop a dual priming oligonucleotide (DPO) based multiplex PCR system for simultaneous detection of shrimp DNA and RNA viruses. This system targets Baculovirus Penaei (BP), Monodon Baculovirus (MB), Infectious Hypodermal, Haematopoietic Necrosis Virus (IHHNV) and White Spot Syndrome Virus (WSSV), Macrobrachium rosenbergii nodavirus (MrNV), Infectious Myonecrosis Virus (IMNV), Taura Syndrome Virus (TSV) and Yellow Head Virus (YHV). The shrimp samples collected from shrimp farm were diagnosed to be infected with YHV infection in the 2nd cycle in year 2018 with 80% screening rate (4/5). DPO based multiplex PCR system may facilitate the rapid and effective screening of shrimp viruses in laboratory.
format Article
author See, SiouNing Aileen
Goh, Zee Hong
Chan, Yen Yew
Chong, Khai En
Tan, Geok Yuan Annie
Bhassu, Subha
Othman, Rofina Yasmin
author_facet See, SiouNing Aileen
Goh, Zee Hong
Chan, Yen Yew
Chong, Khai En
Tan, Geok Yuan Annie
Bhassu, Subha
Othman, Rofina Yasmin
author_sort See, SiouNing Aileen
title Biosafety evaluation and detection of shrimp viruses on field samples using dual priming oligonucleotide (DPO) system based multiplex PCR assay
title_short Biosafety evaluation and detection of shrimp viruses on field samples using dual priming oligonucleotide (DPO) system based multiplex PCR assay
title_full Biosafety evaluation and detection of shrimp viruses on field samples using dual priming oligonucleotide (DPO) system based multiplex PCR assay
title_fullStr Biosafety evaluation and detection of shrimp viruses on field samples using dual priming oligonucleotide (DPO) system based multiplex PCR assay
title_full_unstemmed Biosafety evaluation and detection of shrimp viruses on field samples using dual priming oligonucleotide (DPO) system based multiplex PCR assay
title_sort biosafety evaluation and detection of shrimp viruses on field samples using dual priming oligonucleotide (dpo) system based multiplex pcr assay
publisher Elsevier
publishDate 2021
url http://eprints.um.edu.my/34829/
_version_ 1735409624593465344
score 13.211869

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